Exploring the matrilineal ancestry of swamp buffaloes (Bubalus bubalis) in Sijunjung Regency, West Sumatra, through partial cytochrome-b sequencing.
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| Titel: | Exploring the matrilineal ancestry of swamp buffaloes (Bubalus bubalis) in Sijunjung Regency, West Sumatra, through partial cytochrome-b sequencing. |
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| Autoren: | Ridho, Muhammad1, Cahyadi, Muhammad1, Pramono, Ahmad1, Putra, Widya Pintaka Bayu2, Hartati, Hartati3, Handiwirawan, Eko3, Aprisal, Aprisal4,5, Yulius, Afdal6, Govindarajan, Rasiravathanahalli Kaveriyappan7, Maulana, Muhammad Indra8 mcahyadi@staff.uns.ac.id |
| Quelle: | Open Veterinary Journal. 2025, Vol. 15 Issue 12, p6470-6479. 10p. |
| Publikationsart: | Article |
| Schlagworte: | Haplotypes, Water buffalo, Mitochondrial DNA, Phylogeny, DNA sequencing |
| Geografische Kategorien: | Sumatra (Indonesia), Southeast Asia, East Asia, Indonesia, China |
| Author-Supplied Keywords: | Buffaloes Cyt-b region Matrilineal Sequencing Sijunjung Regency |
| Abstract: | Background: Buffaloes (Bubalus bubalis) are a significant livestock in Indonesia, with approximately 36% (201,060 heads) of their breeding populations located on Sumatera Island, including Sijunjung Regency. Aim: This study aimed to ascertain the matrilineal lineage of swamp buffaloes in Sijunjung Regency, Indonesia, using the mitochondrial cytochrome b (Cyt-b) region. Methods: Blood samples were obtained from 23 adult female swamp buffaloes for DNA extraction and analysis. A total of 906 bp of bubaline Cyt-b amplicons was amplified for forward sequencing. Six bioinformatics programs (BioEdit, DNAsp, MEGA, STRUCTURE, BEAST, and R) were used for sequence alignment, haplotype diversity estimation, phylogenetic tree construction, pairwise genetic distance calculations, and genetic admixture analysis. Results: Seventeen mutation sites were identified in the Cyt-b region. Consequently, four haplotypes (H) of Cyt-b were identified: H1 (56%), H2 (22%), H3 (17%), and H4 (5%). The haplotype diversity (Hd) and nucleotide diversity (pi) for the partial Cyt-b analyzed were 0.645 ± 0.081 and 0.003 ± 0.001, respectively. Phylogenetic tree analysis indicated that the swamp buffaloes in Sijunjung Regency were classified within Haplogroup 1 and exhibited close genetic ties to buffaloes from South Asia (Bangladesh), Southeast Asia (Thailand, Laos, Vietnam, Philippines), and East Asia (Taiwan, China). No evidence of genetic introgression from Indian and Pakistani buffaloes was found in the studied population. Conclusion: The matrilineal origin of buffaloes in Sijunjung Regency may be traced back to the southeastern Chinese buffalo (Lineage A) via two migration routes: the land-bridge (China - IndoChina - Malaya Peninsula - Sumatra) and the insular pathway (China - Taiwan - Philippines - Borneo - Sumatra). [ABSTRACT FROM AUTHOR] |
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| Author Affiliations: | 1Department of Animal Science, Universitas Sebelas Maret, Surakarta, Indonesia 2Research Center for Applied Zoology, National Research and Innovation Agency, Cibinong, Indonesia 3Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong, Indonesia 4Department of Animal Husbandry and Veterinary Medicine, West Sumatera Province, Padang, Indonesia 5Department of Animal Production Technology, Andalas University, Padang, Indonesia 6Department of Procurement, Agency for Regional Development of Sijunjung Regency, Sijunjung, Indonesia 7Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore, India 8Department of Biology, Faculty of Science, Institut Teknologi Sumatra, Lampung, Indonesia |
| ISSN: | 2226-4485 |
| DOI: | 10.5455/OVJ.2025.v15.i12.31 |
| Dokumentencode: | 191964132 |
| Datenbank: | Veterinary Source |
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