Microsatellite-based genetic diversity assessment of Donggala cattle (Bos indicus) in Indonesia: Insights for conservation and breeding.
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| Názov: | Microsatellite-based genetic diversity assessment of Donggala cattle (Bos indicus) in Indonesia: Insights for conservation and breeding. |
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| Autori: | Duma, Yulius1 duma63@yahoo.com, Agung, Paskah Partogi2 paskah_partogi@yahoo.com, Tanari, Mobius1 mobiustanari1567@gmail.com, Malewa, Amirudin Dg.1 amirmalewauntad@gmail.com, Mumu, Muhammad Ilyas1 ilyasmumu@gmail.com, Said, Syahruddin2 syahruddinsaid01@gmail.com, Saputra, Ferdy3 ferdy44saputra@gmail.com, Kaiin, Ekayanti Mulyawati2 ekayantimk@gmail.com, Gunawan, Muhammad2 muhammadgunawan@ymail.com, Maulana, Tulus2 tulus_maul@yahoo.com, Herlina, Nina2 nina.herlina.0212@gmail.com, Damry, Damry1 damry@untad.ac.id |
| Zdroj: | Veterinary World. Oct2025, Vol. 18 Issue 10, p2981-2990. 10p. |
| Druh dokumentu: | Article |
| Predmet: | Genetic variation, Animal breeding, DNA analysis, Environmental protection, Zebus, Microsatellite repeats |
| Geografický termín: | Indonesia, Sulawesi (Indonesia) |
| Author-Supplied Keywords: | 3D principal component analyses breeding conservation Donggala cattle genetic diversity microsatellite markers |
| Abstrakt: | Background and Aim: Donggala cattle (Bos indicus), indigenous to Central Sulawesi, Indonesia, are recognized for their productive and reproductive performance. However, molecular information on their genetic diversity is scarce. Understanding genetic variability is essential for sustainable conservation and targeted breeding strategies. This study aimed to characterize the genetic diversity and relationships of Donggala cattle using microsatellite markers and advanced multivariate analyses. Materials and Methods: Seventy-five blood samples were collected from unrelated Donggala cattle in Central Sulawesi. Genomic DNA was extracted and amplified across ten Food and Agriculture Organization-recommended microsatellite loci. Allele frequency, observed heterozygosity (Ho), expected heterozygosity, and polymorphism information content (PIC) were calculated. Genetic distances and clustering were assessed using Unweighted Pair Group Method with Arithmetic Mean (UPGMA) and analysis of molecular variance (AMOVA). Two- and three-dimensional principal component analysis (PCA) was conducted to visualize genetic differentiation, with comparative datasets from other Indonesian cattle breeds. Results: High allelic diversity was detected, with SPS113 (12 alleles), ETH225 (11 alleles), and TGLA122 (9 alleles) being the most informative markers (PIC: 0.80–0.84). Ho was highest at ETH225 (0.95), reflecting substantial genetic variation. UPGMA and admixture analyses placed Donggala cattle closest to Pesisir cattle, though phenotypically more similar to larger B. indicus breeds such as Ongole Grade. The 3D PCA provided enhanced discriminatory power, distinctly separating Donggala from exotic and crossbred cattle and differentiating Bali cattle from Banteng. AMOVA indicated that 22% of genetic variation existed among populations, while 21% was among individuals. Conclusion: Donggala cattle exhibit considerable genetic diversity, underscoring their value as a reservoir for breeding and conservation programs. Microsatellite markers, particularly SPS113, ETH225, and TGLA122, proved highly informative for genetic assessment. The application of 3D PCA enhanced resolution in distinguishing closely related breeds, supporting its use in molecular characterization. These findings provide essential baseline data for sustainable management, conservation, and genetic improvement of Donggala cattle. [ABSTRACT FROM AUTHOR] |
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| Author Affiliations: | 1Animal Science, Faculty of Animal Husbandry and Fisheries, Tadulako University, Jl. Soekarno Hatta KM. 9, Palu 94117 Central Sulawesi, Indonesia. 2Research Center for Applied Zoology, National Research and Innovation Agency-BRIN, Jl. Raya Bogor KM. 46, Cibinong 16911, West Java, Indonesia. 3Research Center for Animal Husbandry, National Research and Innovation Agency-BRIN, Jl. Raya Bogor KM. 46, Cibinong 16911, West Java, Indonesia. |
| ISSN: | 0972-8988 |
| DOI: | 10.14202/vetworld.2025.2981-2990 |
| Prístupové číslo: | 189111171 |
| Databáza: | Veterinary Source |
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