Frozen semen quality of Pasundan bulls with different individual variations.
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| Název: | Frozen semen quality of Pasundan bulls with different individual variations. |
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| Autoři: | Baharun, Abdullah1 abdullah.baharun@unida.ac.id, Pertiwi, Pirda Parida Permadani1, Rahmi, Annisa1, Rachmadanti, Natashya Chandra1, Handarini, Ristika1, Iskandar, Hikmayani2, Samsudewa, Daud3, Maulana, Tulus4, Said, Syahruddin4, Darussalam, Imam5, Nurcholis, Nurcholis6, Arifiantini, Raden Iis7 |
| Zdroj: | Open Veterinary Journal. 2025, Vol. 15 Issue 1, p244-251. 8p. |
| Druh dokumentu: | Article |
| Témata: | Sperm motility, Artificial insemination, Cattle breeding, Cell membranes, Reproductive health, Frozen semen, Semen analysis, Cattle breeds, Spermatozoa |
| Author-Supplied Keywords: | Comprehensiveevaluation Fluorescent Localbulls Pasundanbull |
| Abstrakt: | Background: Pasundan bulls are one of Indonesia's indigenous cattle breeds. The quality of frozen semen is essential for the success of artificial insemination (AI), as individual variations in parameters such as sperm motility, viability, and membrane integrity greatly influence reproductive outcomes. Aim: The objective of this study was to evaluate the quality of frozen semen from Pasundan bulls with different individual variations. Methods: Frozen semen from eight Pasundan bulls was obtained from the West Java Regional Artificial Insemination Center. Sperm motility was assessed using computer-assisted sperm analysis (CASA; SpermVision®, Germany). Sperm viability and abnormalities were determined by eosin-nigrosine staining. Plasma membrane integrity was evaluated using the hypoosmotic swelling test, acrosome integrity was assessed using fluorescein isothiocyanate-propidium iodide, and protamine deficiency was measured using chromomycin A3 fluorescence staining. Results: Significant individual variations (p < 0.05) were found across all parameters of frozen semen quality, except for sperm abnormalities, which showed no significant differences (p > 0.05). Conclusion: The results showed that individual variations significantly influence all quality parameters of frozen semen from Pasundan bulls, except for sperm abnormalities. Therefore, a thorough assessment of each bull is crucial to ensure the production of quality frozen semen. [ABSTRACT FROM AUTHOR] |
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| Author Affiliations: | 1Department of Animal Science, Faculty of Agriculture, Universitas Djuanda, Bogor, Indonesia 2Research Center for Animal Husbandry, National Research and Innovation Agency (BRIN), Bogor, Indonesia 3Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang, Indonesia 4Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Bogor, Indonesia 5Technical Unit of Bulls Breeding and Artificial Insemination Development Ciamis, Department of Food Security and Livestock, West Java, Indonesia 6Department of Animal Husbandry, Faculty of Animal Agriculture, Musamus University, Papua, Indonesia 7Division of Veterinary Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia |
| ISSN: | 2226-4485 |
| DOI: | 10.5455/OVJ.2025.v15.i1.22 |
| Přístupové číslo: | 183449087 |
| Databáze: | Veterinary Source |
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