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A new method for protein extraction from sea lettuce (Ulva fenestrata) via surfactants and alkaline aqueous solutions

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Názov: A new method for protein extraction from sea lettuce (Ulva fenestrata) via surfactants and alkaline aqueous solutions
Autori: Trigo, João Pedro, 1995, Steinhagen, Sophie, Stedt, Kristoffer, Krona, A., Verhagen, Simone, Pavia, Henrik, 1964, Abdollahi, Mehdi, 1985, Undeland, Ingrid, 1968
Zdroj: Makroalger som bärare av näringsämnen i en cirkulär livsmedelskedja - innovativa steg mot ett proteinskifte (CirkAlg) Food Chemistry. 464
Predmety: Macroalgae, Precipitation, Thylakoid membrane protein, Sustainable food protein, Detergent, Solubility
Popis: Alternative protein sources such as seaweed can help relieve the pressure on land-based protein supply. This proof-of-concept study developed an extraction method to recover soluble and lipophilic proteins from the seaweed Ulva fenestrata. The method consisted of processing U. fenestrata with 0.1–0.5 % aqueous Triton X-114 solution and reprocessing the pellet with an alkaline aqueous solution. Then, the solubilized proteins were precipitated via acidification. The new method extracted 3.4-times more protein, measured as total amino acids, compared to the control with two alkaline aqueous extraction cycles. Triton disrupted the chloroplasts and likely solubilized lipophilic membrane proteins as supported by microstructure and polypeptide pattern analysis. Triton-derived protein extracts contained lipids inside the precipitates/aggregates and were richer in fatty acids typical of photosynthetic membranes. The higher extraction yields are proposed to result from membrane charge neutralization upon acidification, triggering interactions between the membrane lipids and their subsequent precipitation with the lipophilic membrane protein.
Popis súboru: electronic
Prístupová URL adresa: https://research.chalmers.se/publication/543794
https://research.chalmers.se/publication/543794/file/543794_Fulltext.pdf
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Items – Name: Title
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  Data: A new method for protein extraction from sea lettuce (Ulva fenestrata) via surfactants and alkaline aqueous solutions
– Name: Author
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  Data: <searchLink fieldCode="AR" term="%22Trigo%2C+João+Pedro%22">Trigo, João Pedro</searchLink>, 1995<br /><searchLink fieldCode="AR" term="%22Steinhagen%2C+Sophie%22">Steinhagen, Sophie</searchLink><br /><searchLink fieldCode="AR" term="%22Stedt%2C+Kristoffer%22">Stedt, Kristoffer</searchLink><br /><searchLink fieldCode="AR" term="%22Krona%2C+A%2E%22">Krona, A.</searchLink><br /><searchLink fieldCode="AR" term="%22Verhagen%2C+Simone%22">Verhagen, Simone</searchLink><br /><searchLink fieldCode="AR" term="%22Pavia%2C+Henrik%22">Pavia, Henrik</searchLink>, 1964<br /><searchLink fieldCode="AR" term="%22Abdollahi%2C+Mehdi%22">Abdollahi, Mehdi</searchLink>, 1985<br /><searchLink fieldCode="AR" term="%22Undeland%2C+Ingrid%22">Undeland, Ingrid</searchLink>, 1968
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <i>Makroalger som bärare av näringsämnen i en cirkulär livsmedelskedja - innovativa steg mot ett proteinskifte (CirkAlg) Food Chemistry</i>. 464
– Name: Subject
  Label: Subject Terms
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  Data: <searchLink fieldCode="DE" term="%22Macroalgae%22">Macroalgae</searchLink><br /><searchLink fieldCode="DE" term="%22Precipitation%22">Precipitation</searchLink><br /><searchLink fieldCode="DE" term="%22Thylakoid+membrane+protein%22">Thylakoid membrane protein</searchLink><br /><searchLink fieldCode="DE" term="%22Sustainable+food+protein%22">Sustainable food protein</searchLink><br /><searchLink fieldCode="DE" term="%22Detergent%22">Detergent</searchLink><br /><searchLink fieldCode="DE" term="%22Solubility%22">Solubility</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Alternative protein sources such as seaweed can help relieve the pressure on land-based protein supply. This proof-of-concept study developed an extraction method to recover soluble and lipophilic proteins from the seaweed Ulva fenestrata. The method consisted of processing U. fenestrata with 0.1–0.5 % aqueous Triton X-114 solution and reprocessing the pellet with an alkaline aqueous solution. Then, the solubilized proteins were precipitated via acidification. The new method extracted 3.4-times more protein, measured as total amino acids, compared to the control with two alkaline aqueous extraction cycles. Triton disrupted the chloroplasts and likely solubilized lipophilic membrane proteins as supported by microstructure and polypeptide pattern analysis. Triton-derived protein extracts contained lipids inside the precipitates/aggregates and were richer in fatty acids typical of photosynthetic membranes. The higher extraction yields are proposed to result from membrane charge neutralization upon acidification, triggering interactions between the membrane lipids and their subsequent precipitation with the lipophilic membrane protein.
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        Value: 10.1016/j.foodchem.2024.141839
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      – Text: English
    Subjects:
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      – SubjectFull: Precipitation
        Type: general
      – SubjectFull: Thylakoid membrane protein
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              Y: 2025
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