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Alginate and tunicate nanocellulose composite microbeads – Preparation, characterization and cell encapsulation

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Title: Alginate and tunicate nanocellulose composite microbeads – Preparation, characterization and cell encapsulation
Authors: Kjesbu, Joachim S., Zaytseva-Zotova, Daria, Sämfors, Sanna, 1987, Gatenholm, Paul, 1956, Troedsson, Christofer, Thompson, Eric M., Strand, Berit Løkensgard
Source: Carbohydrate Polymers. 286
Subject Terms: Microbeads, Cellulose nanofibrils, Tunicate, Cell encapsulation, Alginate
Description: Alginate has been used for decades for cell encapsulation. Cellulose nanofibrils (CNF) from tunicates are desirable in biomedicine due to high molecular weight, purity, crystallinity, and sustainable production. We prepared microbeads of 400–600 μm of alginate and tunicate CNF. Greater size, dispersity and aspect ratio were observed in microbeads with higher fractions of CNF. CNF content in Ca-crosslinked alginate microbeads decreased stability upon saline exposure, whereas crosslinking with calcium (50 mM) and barium (1 mM) yielded stable microbeads. The Young's moduli of gel cylinders decreased when exchanging alginate with CNF, and slightly increased permeability to dextran was observed in microbeads containing CNF. Encapsulation of MC3T3 cells revealed high cell viability after encapsulation (83.6 ± 0.4%) in beads of alginate and CNF. NHDFs showed lower viability but optimizing mixing and production techniques of microbeads increased cell viability (from 66.2 ± 5.3% to 72.7 ± 7.5%).
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  Data: Alginate and tunicate nanocellulose composite microbeads – Preparation, characterization and cell encapsulation
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  Data: <searchLink fieldCode="AR" term="%22Kjesbu%2C+Joachim+S%2E%22">Kjesbu, Joachim S.</searchLink><br /><searchLink fieldCode="AR" term="%22Zaytseva-Zotova%2C+Daria%22">Zaytseva-Zotova, Daria</searchLink><br /><searchLink fieldCode="AR" term="%22Sämfors%2C+Sanna%22">Sämfors, Sanna</searchLink>, 1987<br /><searchLink fieldCode="AR" term="%22Gatenholm%2C+Paul%22">Gatenholm, Paul</searchLink>, 1956<br /><searchLink fieldCode="AR" term="%22Troedsson%2C+Christofer%22">Troedsson, Christofer</searchLink><br /><searchLink fieldCode="AR" term="%22Thompson%2C+Eric+M%2E%22">Thompson, Eric M.</searchLink><br /><searchLink fieldCode="AR" term="%22Strand%2C+Berit+Løkensgard%22">Strand, Berit Løkensgard</searchLink>
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  Data: <i>Carbohydrate Polymers</i>. 286
– Name: Subject
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  Data: <searchLink fieldCode="DE" term="%22Microbeads%22">Microbeads</searchLink><br /><searchLink fieldCode="DE" term="%22Cellulose+nanofibrils%22">Cellulose nanofibrils</searchLink><br /><searchLink fieldCode="DE" term="%22Tunicate%22">Tunicate</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+encapsulation%22">Cell encapsulation</searchLink><br /><searchLink fieldCode="DE" term="%22Alginate%22">Alginate</searchLink>
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  Label: Description
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  Data: Alginate has been used for decades for cell encapsulation. Cellulose nanofibrils (CNF) from tunicates are desirable in biomedicine due to high molecular weight, purity, crystallinity, and sustainable production. We prepared microbeads of 400–600 μm of alginate and tunicate CNF. Greater size, dispersity and aspect ratio were observed in microbeads with higher fractions of CNF. CNF content in Ca-crosslinked alginate microbeads decreased stability upon saline exposure, whereas crosslinking with calcium (50 mM) and barium (1 mM) yielded stable microbeads. The Young's moduli of gel cylinders decreased when exchanging alginate with CNF, and slightly increased permeability to dextran was observed in microbeads containing CNF. Encapsulation of MC3T3 cells revealed high cell viability after encapsulation (83.6 ± 0.4%) in beads of alginate and CNF. NHDFs showed lower viability but optimizing mixing and production techniques of microbeads increased cell viability (from 66.2 ± 5.3% to 72.7 ± 7.5%).
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      – Type: doi
        Value: 10.1016/j.carbpol.2022.119284
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      – Text: English
    Subjects:
      – SubjectFull: Microbeads
        Type: general
      – SubjectFull: Cellulose nanofibrils
        Type: general
      – SubjectFull: Tunicate
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            NameFull: Thompson, Eric M.
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            NameFull: Strand, Berit Løkensgard
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              Y: 2022
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              Value: 286
          Titles:
            – TitleFull: Carbohydrate Polymers
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