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Energy metabolism controls phenotypes by protein efficiency and allocation

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Název: Energy metabolism controls phenotypes by protein efficiency and allocation
Autoři: Chen, Yu, 1990, Nielsen, Jens B, 1962
Zdroj: Proceedings of the National Academy of Sciences of the United States of America. 116(35):17592-17597
Témata: Saccharomyces cerevisiae, metabolic switch, constraint-based modeling, Escherichia coli, growth rate
Popis: Cells require energy for growth and maintenance and have evolved to have multiple pathways to produce energy in response to varying conditions. A basic question in this context is how cells organize energy metabolism, which is, however, challenging to elucidate due to its complexity, i.e., the energy-producing pathways overlap with each other and even intertwine with biomass formation pathways. Here, we propose a modeling concept that decomposes energy metabolism into biomass formation and ATP-producing pathways. The latter can be further decomposed into a high-yield and a low-yield pathway. This enables independent estimation of protein efficiency for each pathway. With this concept, we modeled energy metabolism for Escherichia coli and Saccharomyces cerevisiae and found that the high-yield pathway shows lower protein efficiency than the low-yield pathway. Taken together with a fixed protein constraint, we predict overflow metabolism in E. coli and the Crabtree effect in S. cerevisiae, meaning that energy metabolism is sufficient to explain the metabolic switches. The static protein constraint is supported by the findings that protein mass of energy metabolism is conserved across conditions based on absolute proteomics data. This also suggests that enzymes may have decreased saturation or activity at low glucose uptake rates. Finally, our analyses point out three ways to improve growth, i.e., increasing protein allocation to energy metabolism, decreasing ATP demand, or increasing activity for key enzymes.
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Přístupová URL adresa: https://research.chalmers.se/publication/512350
https://research.chalmers.se/publication/513352
https://research.chalmers.se/publication/513352/file/513352_Fulltext.pdf
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  Data: Energy metabolism controls phenotypes by protein efficiency and allocation
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  Data: <searchLink fieldCode="AR" term="%22Chen%2C+Yu%22">Chen, Yu</searchLink>, 1990<br /><searchLink fieldCode="AR" term="%22Nielsen%2C+Jens+B%22">Nielsen, Jens B</searchLink>, 1962
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  Data: <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 116(35):17592-17597
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  Data: <searchLink fieldCode="DE" term="%22Saccharomyces+cerevisiae%22">Saccharomyces cerevisiae</searchLink><br /><searchLink fieldCode="DE" term="%22metabolic+switch%22">metabolic switch</searchLink><br /><searchLink fieldCode="DE" term="%22constraint-based+modeling%22">constraint-based modeling</searchLink><br /><searchLink fieldCode="DE" term="%22Escherichia+coli%22">Escherichia coli</searchLink><br /><searchLink fieldCode="DE" term="%22growth+rate%22">growth rate</searchLink>
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  Data: Cells require energy for growth and maintenance and have evolved to have multiple pathways to produce energy in response to varying conditions. A basic question in this context is how cells organize energy metabolism, which is, however, challenging to elucidate due to its complexity, i.e., the energy-producing pathways overlap with each other and even intertwine with biomass formation pathways. Here, we propose a modeling concept that decomposes energy metabolism into biomass formation and ATP-producing pathways. The latter can be further decomposed into a high-yield and a low-yield pathway. This enables independent estimation of protein efficiency for each pathway. With this concept, we modeled energy metabolism for Escherichia coli and Saccharomyces cerevisiae and found that the high-yield pathway shows lower protein efficiency than the low-yield pathway. Taken together with a fixed protein constraint, we predict overflow metabolism in E. coli and the Crabtree effect in S. cerevisiae, meaning that energy metabolism is sufficient to explain the metabolic switches. The static protein constraint is supported by the findings that protein mass of energy metabolism is conserved across conditions based on absolute proteomics data. This also suggests that enzymes may have decreased saturation or activity at low glucose uptake rates. Finally, our analyses point out three ways to improve growth, i.e., increasing protein allocation to energy metabolism, decreasing ATP demand, or increasing activity for key enzymes.
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