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Strain-Specific Bacillus subtilis-Derived Silver Nanoparticles for Effective Antibacterial Activity Against Multidrug-Resistant Pathogens: In Vitro Model

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Název: Strain-Specific Bacillus subtilis-Derived Silver Nanoparticles for Effective Antibacterial Activity Against Multidrug-Resistant Pathogens: In Vitro Model
Autoři: Singh, Priyanka, Mijakovic, Ivan, 1975
Zdroj: International journal of nanomedicine. 20:13055-13078
Témata: silver nanoparticles, antimicrobial resistance, simultaneous production, B. subtilis, multidrug-resistant pathogens, gold nanoparticles, stability
Popis: Introduction: Nanoparticles, particularly silver and gold nanoparticles, have emerged as powerful tools for improving drug stability, bioavailability, and targeted delivery in medical applications. With the increasing global demand for nanoparticles (NPs), sustainable and biocompatible production methods are crucial. This study investigates the biosynthesis of silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) using two genetically related bacterial strains, Bacillus subtilis 168 and Bacillus subtilis 3610, and compares their synthesis efficiency and the antimicrobial potential of the generated NPs. Methods: AgNPs were synthesized extracellularly from bacterial cell supernatants, whereas AuNPs were generated intracellularly by incubating washed cell pellets with gold salt. The nanoparticles were characterized using UV–Vis spectroscopy, sp-ICP-MS, TGA, SEM, TEM, FTIR, MALDI-TOF, zeta potential, and AFM. Antibacterial activity against Pseudomonas aeruginosa and Escherichia coli was evaluated using minimum bactericidal concentration (MBC) assays, live/dead fluorescence staining, and SEM-based morphological analysis. Results: Both strains synthesized AgNPs and AuNPs, but Bacillus subtilis 3610 exhibited faster and more efficient production. AgNPs displayed uniform spherical morphology, whereas AuNPs were polydisperse and membrane-associated. AgNPs synthesis was completed within 2 days by strain 3610 compared with 3 days by strain 168. Antibacterial assays revealed markedly stronger efficacy of 3610-derived AgNPs, with MBC values of 2 µg/mL against P. aeruginosa and 8 µg/mL against E. coli, in contrast to 16 µg/mL and 32 µg/mL, respectively, for 168-derived AgNPs. Conclusion: This study highlights the superior NPs biosynthesis capacity and antibacterial potency of the wild-type strain B. subtilis 3610 compared to the domesticated laboratory strain 168. The findings emphasize the critical influence of strain-specific genetic and metabolic traits in optimizing microbial nanomaterial production and position 3610-derived AgNPs as promising candidates for biomedical applications.
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Strain-Specific Bacillus subtilis-Derived Silver Nanoparticles for Effective Antibacterial Activity Against Multidrug-Resistant Pathogens: In Vitro Model
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Singh%2C+Priyanka%22">Singh, Priyanka</searchLink><br /><searchLink fieldCode="AR" term="%22Mijakovic%2C+Ivan%22">Mijakovic, Ivan</searchLink>, 1975
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  Data: <i>International journal of nanomedicine</i>. 20:13055-13078
– Name: Subject
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  Data: <searchLink fieldCode="DE" term="%22silver+nanoparticles%22">silver nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22antimicrobial+resistance%22">antimicrobial resistance</searchLink><br /><searchLink fieldCode="DE" term="%22simultaneous+production%22">simultaneous production</searchLink><br /><searchLink fieldCode="DE" term="%22B%2E+subtilis%22">B. subtilis</searchLink><br /><searchLink fieldCode="DE" term="%22multidrug-resistant+pathogens%22">multidrug-resistant pathogens</searchLink><br /><searchLink fieldCode="DE" term="%22gold+nanoparticles%22">gold nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22stability%22">stability</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Introduction: Nanoparticles, particularly silver and gold nanoparticles, have emerged as powerful tools for improving drug stability, bioavailability, and targeted delivery in medical applications. With the increasing global demand for nanoparticles (NPs), sustainable and biocompatible production methods are crucial. This study investigates the biosynthesis of silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) using two genetically related bacterial strains, Bacillus subtilis 168 and Bacillus subtilis 3610, and compares their synthesis efficiency and the antimicrobial potential of the generated NPs. Methods: AgNPs were synthesized extracellularly from bacterial cell supernatants, whereas AuNPs were generated intracellularly by incubating washed cell pellets with gold salt. The nanoparticles were characterized using UV–Vis spectroscopy, sp-ICP-MS, TGA, SEM, TEM, FTIR, MALDI-TOF, zeta potential, and AFM. Antibacterial activity against Pseudomonas aeruginosa and Escherichia coli was evaluated using minimum bactericidal concentration (MBC) assays, live/dead fluorescence staining, and SEM-based morphological analysis. Results: Both strains synthesized AgNPs and AuNPs, but Bacillus subtilis 3610 exhibited faster and more efficient production. AgNPs displayed uniform spherical morphology, whereas AuNPs were polydisperse and membrane-associated. AgNPs synthesis was completed within 2 days by strain 3610 compared with 3 days by strain 168. Antibacterial assays revealed markedly stronger efficacy of 3610-derived AgNPs, with MBC values of 2 µg/mL against P. aeruginosa and 8 µg/mL against E. coli, in contrast to 16 µg/mL and 32 µg/mL, respectively, for 168-derived AgNPs. Conclusion: This study highlights the superior NPs biosynthesis capacity and antibacterial potency of the wild-type strain B. subtilis 3610 compared to the domesticated laboratory strain 168. The findings emphasize the critical influence of strain-specific genetic and metabolic traits in optimizing microbial nanomaterial production and position 3610-derived AgNPs as promising candidates for biomedical applications.
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        Value: 10.2147/IJN.S527950
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      – SubjectFull: silver nanoparticles
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      – SubjectFull: antimicrobial resistance
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      – SubjectFull: simultaneous production
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      – TitleFull: Strain-Specific Bacillus subtilis-Derived Silver Nanoparticles for Effective Antibacterial Activity Against Multidrug-Resistant Pathogens: In Vitro Model
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            NameFull: Mijakovic, Ivan
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              Y: 2025
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