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MicroRNA-379 Modulates Prostate-Specific Antigen Expression Through Targeting the Androgen Receptor in Prostate Cancer

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Titel: MicroRNA-379 Modulates Prostate-Specific Antigen Expression Through Targeting the Androgen Receptor in Prostate Cancer
Autoren: Cassidy, James R, Persson, Margareta, Voss, Gjendine, Underbjerg, Kira Rosenkilde, Ivkovic, Tina Catela, Bjartell, Anders, Edsjö, Anders, Lilja, Hans, Ceder, Yvonne
Weitere Verfasser: Lund University, Faculty of Medicine, Department of Clinical Sciences, Lund, Section I, Medical Molecular Biology, Lunds universitet, Medicinska fakulteten, Institutionen för kliniska vetenskaper, Lund, Sektion I, Medicinsk molekylärbiologi, Originator, Lund University, Profile areas and other strong research environments, Other Strong Research Environments, LUCC: Lund University Cancer Centre, Lunds universitet, Profilområden och andra starka forskningsmiljöer, Övriga starka forskningsmiljöer, LUCC: Lunds universitets cancercentrum, Originator, Lund University, Faculty of Medicine, Department of Laboratory Medicine, Division of Translational Cancer Research, Medical Molecular Biology, Lunds universitet, Medicinska fakulteten, Institutionen för laboratoriemedicin, Avdelningen för translationell cancerforskning, Medicinsk molekylärbiologi, Originator, Lund University, Faculty of Medicine, Department of Experimental Medical Science, Molecular Evolution, Lunds universitet, Medicinska fakulteten, Institutionen för experimentell medicinsk vetenskap, Molekylär evolution, Originator, Lund University, Faculty of Medicine, Department of Laboratory Medicine, Division of Clinical Genetics, Center for Translational Genomics (CTG), Lunds universitet, Medicinska fakulteten, Institutionen för laboratoriemedicin, Avdelningen för klinisk genetik, Centrum för Translationell Genomik (CTG), Originator, Lund University, Faculty of Medicine, Department of Laboratory Medicine, Division of Clinical Genetics, Lunds universitet, Medicinska fakulteten, Institutionen för laboratoriemedicin, Avdelningen för klinisk genetik, Originator, Lund University, Faculty of Medicine, Department of Translational Medicine, Clinical Chemistry, Malmö, Lunds universitet, Medicinska fakulteten, Institutionen för translationell medicin, Klinisk kemi, Malmö, Originator, Lund University, Profile areas and other strong research environments, Strategic research areas (SRA), EpiHealth: Epidemiology for Health, Lunds universitet, Profilområden och andra starka forskningsmiljöer, Strategiska forskningsområden (SFO), EpiHealth: Epidemiology for Health, Originator, Lund University, Faculty of Medicine, Department of Laboratory Medicine, Division of Translational Cancer Research, Lunds universitet, Medicinska fakulteten, Institutionen för laboratoriemedicin, Avdelningen för translationell cancerforskning, Originator
Quelle: Cancers. 17(19):1-13
Schlagwörter: Medical and Health Sciences, Clinical Medicine, Cancer and Oncology, Medicin och hälsovetenskap, Klinisk medicin, Cancer och onkologi, Urology, Urologi
Beschreibung: Background: MicroRNA-379 (miR-379) has been reported to play a tumour-suppressing role in several cancer types. Our previous work demonstrated that miR-379 overexpression attenuates the metastatic spread of prostate cancer (PCa) both in vitro and in vivo. However, the underlying mechanisms remain poorly understood. Methods: To elucidate the mechanisms by which miR-379 affects metastases, we performed a cytokine array to identify secreted proteins modulated by miR-379 dysregulation in a bone microenvironment model. We then assessed the levels of the key candidate, and performed functional studies, including reporter assays, of the transcriptional regulation. Results: Prostate-specific antigen (PSA)-the clinically widely used blood biomarker for PCa-emerged as the most significantly affected secreted protein. We observed that PSA secretion increased following miR-379 inhibition and decreased with miR-379 overexpression, with parallel changes in intracellular PSA levels. However, our data suggests that miR-379 does not directly regulate PSA expression. Instead, miR-379 appears to downregulate androgen receptor (AR) expression by targeting its 3'-untranslated region (3'-UTR), thereby indirectly reducing PSA transcription through diminished AR-mediated promoter activation. Supporting this indirect mechanism, analysis of clinical samples from prostate cancer patients revealed an inverse correlation between expression of miR-379 in prostatic tissue and serum PSA levels. Furthermore, reduced miR-379 expression was associated with increased levels of AR immunostaining in malignant tissues. Conclusions: Taken together, these findings suggest that miR-379 negatively regulates PSA secretion indirectly via suppression of AR, and that the interplay between miR-379, AR, and PSA may contribute to the metastatic progression of PCa to bone.
Zugangs-URL: https://doi.org/10.3390/cancers17193245
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  Data: MicroRNA-379 Modulates Prostate-Specific Antigen Expression Through Targeting the Androgen Receptor in Prostate Cancer
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  Data: <searchLink fieldCode="AR" term="%22Cassidy%2C+James+R%22">Cassidy, James R</searchLink><br /><searchLink fieldCode="AR" term="%22Persson%2C+Margareta%22">Persson, Margareta</searchLink><br /><searchLink fieldCode="AR" term="%22Voss%2C+Gjendine%22">Voss, Gjendine</searchLink><br /><searchLink fieldCode="AR" term="%22Underbjerg%2C+Kira+Rosenkilde%22">Underbjerg, Kira Rosenkilde</searchLink><br /><searchLink fieldCode="AR" term="%22Ivkovic%2C+Tina+Catela%22">Ivkovic, Tina Catela</searchLink><br /><searchLink fieldCode="AR" term="%22Bjartell%2C+Anders%22">Bjartell, Anders</searchLink><br /><searchLink fieldCode="AR" term="%22Edsjö%2C+Anders%22">Edsjö, Anders</searchLink><br /><searchLink fieldCode="AR" term="%22Lilja%2C+Hans%22">Lilja, Hans</searchLink><br /><searchLink fieldCode="AR" term="%22Ceder%2C+Yvonne%22">Ceder, Yvonne</searchLink>
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  Data: Lund University, Faculty of Medicine, Department of Clinical Sciences, Lund, Section I, Medical Molecular Biology, Lunds universitet, Medicinska fakulteten, Institutionen för kliniska vetenskaper, Lund, Sektion I, Medicinsk molekylärbiologi, Originator<br />Lund University, Profile areas and other strong research environments, Other Strong Research Environments, LUCC: Lund University Cancer Centre, Lunds universitet, Profilområden och andra starka forskningsmiljöer, Övriga starka forskningsmiljöer, LUCC: Lunds universitets cancercentrum, Originator<br />Lund University, Faculty of Medicine, Department of Laboratory Medicine, Division of Translational Cancer Research, Medical Molecular Biology, Lunds universitet, Medicinska fakulteten, Institutionen för laboratoriemedicin, Avdelningen för translationell cancerforskning, Medicinsk molekylärbiologi, Originator<br />Lund University, Faculty of Medicine, Department of Experimental Medical Science, Molecular Evolution, Lunds universitet, Medicinska fakulteten, Institutionen för experimentell medicinsk vetenskap, Molekylär evolution, Originator<br />Lund University, Faculty of Medicine, Department of Laboratory Medicine, Division of Clinical Genetics, Center for Translational Genomics (CTG), Lunds universitet, Medicinska fakulteten, Institutionen för laboratoriemedicin, Avdelningen för klinisk genetik, Centrum för Translationell Genomik (CTG), Originator<br />Lund University, Faculty of Medicine, Department of Laboratory Medicine, Division of Clinical Genetics, Lunds universitet, Medicinska fakulteten, Institutionen för laboratoriemedicin, Avdelningen för klinisk genetik, Originator<br />Lund University, Faculty of Medicine, Department of Translational Medicine, Clinical Chemistry, Malmö, Lunds universitet, Medicinska fakulteten, Institutionen för translationell medicin, Klinisk kemi, Malmö, Originator<br />Lund University, Profile areas and other strong research environments, Strategic research areas (SRA), EpiHealth: Epidemiology for Health, Lunds universitet, Profilområden och andra starka forskningsmiljöer, Strategiska forskningsområden (SFO), EpiHealth: Epidemiology for Health, Originator<br />Lund University, Faculty of Medicine, Department of Laboratory Medicine, Division of Translational Cancer Research, Lunds universitet, Medicinska fakulteten, Institutionen för laboratoriemedicin, Avdelningen för translationell cancerforskning, Originator
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  Data: <i>Cancers</i>. 17(19):1-13
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  Data: Background: MicroRNA-379 (miR-379) has been reported to play a tumour-suppressing role in several cancer types. Our previous work demonstrated that miR-379 overexpression attenuates the metastatic spread of prostate cancer (PCa) both in vitro and in vivo. However, the underlying mechanisms remain poorly understood. Methods: To elucidate the mechanisms by which miR-379 affects metastases, we performed a cytokine array to identify secreted proteins modulated by miR-379 dysregulation in a bone microenvironment model. We then assessed the levels of the key candidate, and performed functional studies, including reporter assays, of the transcriptional regulation. Results: Prostate-specific antigen (PSA)-the clinically widely used blood biomarker for PCa-emerged as the most significantly affected secreted protein. We observed that PSA secretion increased following miR-379 inhibition and decreased with miR-379 overexpression, with parallel changes in intracellular PSA levels. However, our data suggests that miR-379 does not directly regulate PSA expression. Instead, miR-379 appears to downregulate androgen receptor (AR) expression by targeting its 3'-untranslated region (3'-UTR), thereby indirectly reducing PSA transcription through diminished AR-mediated promoter activation. Supporting this indirect mechanism, analysis of clinical samples from prostate cancer patients revealed an inverse correlation between expression of miR-379 in prostatic tissue and serum PSA levels. Furthermore, reduced miR-379 expression was associated with increased levels of AR immunostaining in malignant tissues. Conclusions: Taken together, these findings suggest that miR-379 negatively regulates PSA secretion indirectly via suppression of AR, and that the interplay between miR-379, AR, and PSA may contribute to the metastatic progression of PCa to bone.
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