Acoustophoresis-based blood sampling and plasma separation for potentially minimizing sampling-related blood loss
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| Title: | Acoustophoresis-based blood sampling and plasma separation for potentially minimizing sampling-related blood loss |
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| Authors: | Nath, Amal, Larsson, Sara Marie, Lenshof, Andreas, Qiu, Wei, Baasch, Thierry, Nilsson, Linda, Gram, Magnus, Ley, David, Laurell, Thomas |
| Source: | Clinical Chemistry and Laboratory Medicine. 63(11):2218-2225 |
| Subject Terms: | acoustofluidics, analytes, blood sampling, clinical chemistry, plasmapheresis, pre-Analytics |
| Description: | Frequent blood sampling in vulnerable patient groups, such as prematurely born infants, can lead to significant blood loss and increased transfusion needs. Current pre-Analytical technology requires comparably large blood volumes and leads to discarding of cells. This study investigates a device prototype enabling in-line sampling where cell-reduced plasma for clinical chemistry analyses is generated through acoustophoresis. Blood samples were collected from healthy adult donors in lithium-heparin tubes without gel. Plasma separated via acoustophoresis was compared with centrifuged plasma (2000g × 10min) for cell counts (n=14), cell-free hemoglobin (n=21), and 12 routine clinical chemistry analyte tests (n=21). Wilcoxon signed-rank tests and Bland Altman analysis were used for statistical comparison. Both acoustophoresis (AF) and centrifugation (CEN) generated cell-reduced plasma with<0.01% of cells remaining after separation. However, compared to CEN plasma, more cells (median count per μL 642 vs. 205, p<0.01) and platelets (median count per μL 20,477 vs. 1,537, p<0.0001) remained in AF plasma. Cell-free hemoglobin (fHb) in AF plasma samples (range 0.0-0.2g/L) was lower (p<0.01) than in CEN plasma samples (range 0.1-0.3g/L). Statistically significant relative mean differences in test results ranging from 0.84% (95% CI 0.48-1.19) for sodium to 10.50% (95% CI 5.02-15.99) for AST were found. This proof-of-concept study demonstrates that acoustophoresis has the potential to produce sufficiently cell-free plasma for several commonly performed clinical chemistry analyses. Further studies should assess pathological samples, platelet activation, and improve the design for more efficient removal of platelets. |
| File Description: | electronic |
| Access URL: | https://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-78824 https://doi.org/10.1515/cclm-2025-0539 |
| Database: | SwePub |
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