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Resistance-associated polymorphisms in Dutch hepatitis C genotype 1a patients with and without HIV infection

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Název: Resistance-associated polymorphisms in Dutch hepatitis C genotype 1a patients with and without HIV infection
Autoři: Faydra I. Lieveld, Niels Swaans, Astrid M. Newsum, Cynthia K.Y. Ho, Janke Schinkel, Richard Molenkamp, Jan T.M. van der Meer, Joop E. Arends, Andy I.M. Hoepelman, Anne M.J. Wensing, Peter D. Siersema, Karel J. van Erpecum, Greet J. Boland
Zdroj: Annals of Hepatology, Vol 15, Iss 5, Pp 696-704 (2016)
Informace o vydavateli: Elsevier, 2016.
Rok vydání: 2016
Sbírka: LCC:Specialties of internal medicine
Témata: Hepatitis C virus, HIV, Genotype 1a, Q80K, Drug resistance, Specialties of internal medicine, RC581-951
Popis: Background and aim. Resistance-associated variants (RAVs) on the NS3 region of the hepatitis C virus (HCV) may be relevant for antiviral therapy, but data in human immunodeficiency virus (HIV) coinfected patients are scarce. We assessed frequencies of NS3 RAVs in patients infected with HCV genotype 1a with or without HIV coinfection.Material and methods. HCV NS3 amino acids 1-181 were sequenced by the Sanger method and analyzed for RAVs. RAVs and their distribution between HCV genotype 1a clade I and II viruses were compared between HIV-infected versus HIV-uninfected patients.Results. 148 samples were available (n = 68 HIV and n = 80 non-HIV). Relative frequency of clade I and clade II was significantly different between HIV (85% and 15%) and non-HIV groups (49% and 51%). Overall, HIV infected patients exhibited significantly lower prevalence of RAVs than HIV-uninfected patients (62% vs. 79%, p = 0.03). However, Q80K prevalence was significantly higher in HIV-infected subjects (50% vs. 24%, p = 0.001), whereas prevalence of S122D/G/N/S (2% vs. 16%, p = 0.002) and N174G/N/S (10% vs. 55%, p < 0.0001) polymorphisms were significantly lower. Q80K was found exclusively in clade I viruses. S122 (3% vs. 22%, p=0.001) and N174 (13% vs. 75%, p
Druh dokumentu: article
Popis souboru: electronic resource
Jazyk: English
ISSN: 1665-2681
Relation: http://www.sciencedirect.com/science/article/pii/S1665268119306313; https://doaj.org/toc/1665-2681
DOI: 10.5604/16652681.1212426
Přístupová URL adresa: https://doaj.org/article/ef7f7c7b6ddf4c0f9a907447d000c29e
Přístupové číslo: edsdoj.f7f7c7b6ddf4c0f9a907447d000c29e
Databáze: Directory of Open Access Journals
Popis
Abstrakt:Background and aim. Resistance-associated variants (RAVs) on the NS3 region of the hepatitis C virus (HCV) may be relevant for antiviral therapy, but data in human immunodeficiency virus (HIV) coinfected patients are scarce. We assessed frequencies of NS3 RAVs in patients infected with HCV genotype 1a with or without HIV coinfection.Material and methods. HCV NS3 amino acids 1-181 were sequenced by the Sanger method and analyzed for RAVs. RAVs and their distribution between HCV genotype 1a clade I and II viruses were compared between HIV-infected versus HIV-uninfected patients.Results. 148 samples were available (n = 68 HIV and n = 80 non-HIV). Relative frequency of clade I and clade II was significantly different between HIV (85% and 15%) and non-HIV groups (49% and 51%). Overall, HIV infected patients exhibited significantly lower prevalence of RAVs than HIV-uninfected patients (62% vs. 79%, p = 0.03). However, Q80K prevalence was significantly higher in HIV-infected subjects (50% vs. 24%, p = 0.001), whereas prevalence of S122D/G/N/S (2% vs. 16%, p = 0.002) and N174G/N/S (10% vs. 55%, p < 0.0001) polymorphisms were significantly lower. Q80K was found exclusively in clade I viruses. S122 (3% vs. 22%, p=0.001) and N174 (13% vs. 75%, p
ISSN:16652681
DOI:10.5604/16652681.1212426