View in EDS

The Identification of FN1 as an Early Diagnostic Marker for Recurrent Abortion by Single-Exosome Profiling

Saved in:
Bibliographic Details
Title: The Identification of FN1 as an Early Diagnostic Marker for Recurrent Abortion by Single-Exosome Profiling
Authors: Wang C, Lu Z, She G, Chen K, Zhou H, Zhan X, Yu H, Pi L, Zuo L, Che D
Source: International Journal of General Medicine, Vol 18, Pp 691-702 (2025)
Publisher Information: Dove Medical Press, 2025.
Publication Year: 2025
Collection: LCC:Medicine (General)
Subject Terms: recurrent abortion，exosomes，fn1，pba, Medicine (General), R5-920
Description: Chenlu Wang,1,* Zhaojin Lu,1,* Guangpeng She,2 Kaining Chen,1 Huazhong Zhou,1 Xueli Zhan,3 Hongyan Yu,1 Lei Pi,1 Liandong Zuo,4 Di Che1 1Department of Clinical Biological Resource Bank, Guangzhou Institute of Pediatrics, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, 510620, People’s Republic of China; 2Department of Laboratory Medicine, The Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong, 510180, People’s Republic of China; 3Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, 510620, People’s Republic of China; 4Department of Andrology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, 510620, People’s Republic of China*These authors contributed equally to this workCorrespondence: Di Che, Department of Clinical Biological Resource Bank, Guangzhou Institute of Pediatrics, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, No. 9 Jinsui Road, Guangzhou, Guangdong, 510620, People’s Republic of China, Email chedi@gwcmc.org Liandong Zuo, Department of Andrology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, 9 Jinsui Road, Guangzhou, Guangdong, 510620, People’s Republic of China, Email zuold@163.comPurpose: Recurrent abortion(RA) is a prevalent adverse pregnancy event. Exosomes, secreted by various body fluids, are known to play a role in disease diagnosis and serve as biomarkers through intercellular communication. This study aims to analyze single exosomes in patients with recurrent abortion to identify new biomarkers that may significantly contribute to recurrent abortion, providing new directions for its treatment.Patients and Methods: A total of 244 serum exosomes were collected, including 216 patients with recurrent abortion of varying outcomes and 28 normal pregnancies. We performed the proximity barcoding assay (PBA) to analyze single exosome surface proteins, which allowed us to identify individual exosomes related to the development of RA as well as the major subpopulations of exosomes. After PBA treatment, samples were analyzed for single exosomes, and exosomes from each group were compared using volcano plots, dot plots, and ROC curves.Results: By intersecting all significantly differentially expressed genes obtained from comparisons between the normal pregnancy control group and the recurrent abortion group, including the RA before abortion, RA after abortion, and RA non-pregnancy groups, we identified seven shared differential genes: FN1, APIPOQ, CDH13, DSG1, CLDN4, CD36, and ULBP3. Among these, FN1 was the most significantly differentially expressed gene in exosomes, with FN1 | log2 (fold change) |> 1.5 and an AUC of 0.7414. In addition, exosome subpopulation analyses showed that cluster 11 accounted for the largest proportion of the total 16 subpopulations, and FN1 was the marker with the highest concentration of cluster 11.Conclusion: Single-exosome profiling and exosome subpopulations of RA by PBA yielded significant differential gene FN1, which provides new possibilities for diagnostic screening of RA.Keywords: recurrent abortion, exosomes, FN1, PBA
Document Type: article
File Description: electronic resource
Language: English
ISSN: 1178-7074
Relation: https://www.dovepress.com/the-identification-of-fn1-as-an-early-diagnostic-marker-for-recurrent--peer-reviewed-fulltext-article-IJGM; https://doaj.org/toc/1178-7074
Access URL: https://doaj.org/article/6afb1d67c21e4eb18b51f54d586a5ec6
Accession Number: edsdoj.6afb1d67c21e4eb18b51f54d586a5ec6
Database: Directory of Open Access Journals
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://doaj.org/article/6afb1d67c21e4eb18b51f54d586a5ec6
    Name: EDS - DOAJ (s4221598)
    Category: fullText
    Text: View record in DOAJ
  – Url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&db=pmc&term=1178-7074[TA]+AND+691[PG]+AND+2025[PDAT]
    Name: FREE - PubMed Central (ISSN based link)
    Category: fullText
    Text: Full Text
    Icon: https://imageserver.ebscohost.com/NetImages/iconPdf.gif
    MouseOverText: Check this PubMed for the article full text.
  – Url: https://resolver.ebscohost.com/openurl?sid=EBSCO:edsdoj&genre=article&issn=11787074&ISBN=&volume=18&issue=&date=20250201&spage=691&pages=691-702&title=International Journal of General Medicine&atitle=The%20Identification%20of%20FN1%20as%20an%20Early%20Diagnostic%20Marker%20for%20Recurrent%20Abortion%20by%20Single-Exosome%20Profiling&aulast=Wang%20C&id=DOI:
    Name: Full Text Finder
    Category: fullText
    Text: Full Text Finder
    Icon: https://imageserver.ebscohost.com/branding/images/FTF.gif
    MouseOverText: Full Text Finder
  – Url: https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=EBSCO&SrcAuth=EBSCO&DestApp=WOS&ServiceName=TransferToWoS&DestLinkType=GeneralSearchSummary&Func=Links&author=C%20W
    Name: ISI
    Category: fullText
    Text: Nájsť tento článok vo Web of Science
    Icon: https://imagesrvr.epnet.com/ls/20docs.gif
    MouseOverText: Nájsť tento článok vo Web of Science
Header DbId: edsdoj
DbLabel: Directory of Open Access Journals
An: edsdoj.6afb1d67c21e4eb18b51f54d586a5ec6
RelevancyScore: 1034
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 1034.31896972656
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: The Identification of FN1 as an Early Diagnostic Marker for Recurrent Abortion by Single-Exosome Profiling
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Wang+C%22">Wang C</searchLink><br /><searchLink fieldCode="AR" term="%22Lu+Z%22">Lu Z</searchLink><br /><searchLink fieldCode="AR" term="%22She+G%22">She G</searchLink><br /><searchLink fieldCode="AR" term="%22Chen+K%22">Chen K</searchLink><br /><searchLink fieldCode="AR" term="%22Zhou+H%22">Zhou H</searchLink><br /><searchLink fieldCode="AR" term="%22Zhan+X%22">Zhan X</searchLink><br /><searchLink fieldCode="AR" term="%22Yu+H%22">Yu H</searchLink><br /><searchLink fieldCode="AR" term="%22Pi+L%22">Pi L</searchLink><br /><searchLink fieldCode="AR" term="%22Zuo+L%22">Zuo L</searchLink><br /><searchLink fieldCode="AR" term="%22Che+D%22">Che D</searchLink>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: International Journal of General Medicine, Vol 18, Pp 691-702 (2025)
– Name: Publisher
  Label: Publisher Information
  Group: PubInfo
  Data: Dove Medical Press, 2025.
– Name: DatePubCY
  Label: Publication Year
  Group: Date
  Data: 2025
– Name: Subset
  Label: Collection
  Group: HoldingsInfo
  Data: LCC:Medicine (General)
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22recurrent+abortion，exosomes，fn1，pba%22">recurrent abortion，exosomes，fn1，pba</searchLink><br /><searchLink fieldCode="DE" term="%22Medicine+%28General%29%22">Medicine (General)</searchLink><br /><searchLink fieldCode="DE" term="%22R5-920%22">R5-920</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Chenlu Wang,1,* Zhaojin Lu,1,* Guangpeng She,2 Kaining Chen,1 Huazhong Zhou,1 Xueli Zhan,3 Hongyan Yu,1 Lei Pi,1 Liandong Zuo,4 Di Che1 1Department of Clinical Biological Resource Bank, Guangzhou Institute of Pediatrics, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, 510620, People’s Republic of China; 2Department of Laboratory Medicine, The Second Affiliated Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong, 510180, People’s Republic of China; 3Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, 510620, People’s Republic of China; 4Department of Andrology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, 510620, People’s Republic of China*These authors contributed equally to this workCorrespondence: Di Che, Department of Clinical Biological Resource Bank, Guangzhou Institute of Pediatrics, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, No. 9 Jinsui Road, Guangzhou, Guangdong, 510620, People’s Republic of China, Email chedi@gwcmc.org Liandong Zuo, Department of Andrology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, 9 Jinsui Road, Guangzhou, Guangdong, 510620, People’s Republic of China, Email zuold@163.comPurpose: Recurrent abortion(RA) is a prevalent adverse pregnancy event. Exosomes, secreted by various body fluids, are known to play a role in disease diagnosis and serve as biomarkers through intercellular communication. This study aims to analyze single exosomes in patients with recurrent abortion to identify new biomarkers that may significantly contribute to recurrent abortion, providing new directions for its treatment.Patients and Methods: A total of 244 serum exosomes were collected, including 216 patients with recurrent abortion of varying outcomes and 28 normal pregnancies. We performed the proximity barcoding assay (PBA) to analyze single exosome surface proteins, which allowed us to identify individual exosomes related to the development of RA as well as the major subpopulations of exosomes. After PBA treatment, samples were analyzed for single exosomes, and exosomes from each group were compared using volcano plots, dot plots, and ROC curves.Results: By intersecting all significantly differentially expressed genes obtained from comparisons between the normal pregnancy control group and the recurrent abortion group, including the RA before abortion, RA after abortion, and RA non-pregnancy groups, we identified seven shared differential genes: FN1, APIPOQ, CDH13, DSG1, CLDN4, CD36, and ULBP3. Among these, FN1 was the most significantly differentially expressed gene in exosomes, with FN1 | log2 (fold change) |> 1.5 and an AUC of 0.7414. In addition, exosome subpopulation analyses showed that cluster 11 accounted for the largest proportion of the total 16 subpopulations, and FN1 was the marker with the highest concentration of cluster 11.Conclusion: Single-exosome profiling and exosome subpopulations of RA by PBA yielded significant differential gene FN1, which provides new possibilities for diagnostic screening of RA.Keywords: recurrent abortion, exosomes, FN1, PBA
– Name: TypeDocument
  Label: Document Type
  Group: TypDoc
  Data: article
– Name: Format
  Label: File Description
  Group: SrcInfo
  Data: electronic resource
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: ISSN
  Label: ISSN
  Group: ISSN
  Data: 1178-7074
– Name: NoteTitleSource
  Label: Relation
  Group: SrcInfo
  Data: https://www.dovepress.com/the-identification-of-fn1-as-an-early-diagnostic-marker-for-recurrent--peer-reviewed-fulltext-article-IJGM; https://doaj.org/toc/1178-7074
– Name: URL
  Label: Access URL
  Group: URL
  Data: <link linkTarget="URL" linkTerm="https://doaj.org/article/6afb1d67c21e4eb18b51f54d586a5ec6" linkWindow="_blank">https://doaj.org/article/6afb1d67c21e4eb18b51f54d586a5ec6</link>
– Name: AN
  Label: Accession Number
  Group: ID
  Data: edsdoj.6afb1d67c21e4eb18b51f54d586a5ec6
PLink https://erproxy.cvtisr.sk/sfx/access?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsdoj&AN=edsdoj.6afb1d67c21e4eb18b51f54d586a5ec6
RecordInfo BibRecord:
  BibEntity:
    Languages:
      – Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 12
        StartPage: 691
    Subjects:
      – SubjectFull: recurrent abortion，exosomes，fn1，pba
        Type: general
      – SubjectFull: Medicine (General)
        Type: general
      – SubjectFull: R5-920
        Type: general
    Titles:
      – TitleFull: The Identification of FN1 as an Early Diagnostic Marker for Recurrent Abortion by Single-Exosome Profiling
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Wang C
      – PersonEntity:
          Name:
            NameFull: Lu Z
      – PersonEntity:
          Name:
            NameFull: She G
      – PersonEntity:
          Name:
            NameFull: Chen K
      – PersonEntity:
          Name:
            NameFull: Zhou H
      – PersonEntity:
          Name:
            NameFull: Zhan X
      – PersonEntity:
          Name:
            NameFull: Yu H
      – PersonEntity:
          Name:
            NameFull: Pi L
      – PersonEntity:
          Name:
            NameFull: Zuo L
      – PersonEntity:
          Name:
            NameFull: Che D
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 02
              Type: published
              Y: 2025
          Identifiers:
            – Type: issn-print
              Value: 11787074
          Numbering:
            – Type: volume
              Value: 18
          Titles:
            – TitleFull: International Journal of General Medicine
              Type: main
ResultId 1