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Dexmedetomidine Alleviates Hippocampal Tissue Damage in Rapid Eye Movement Sleep-Deprived Rats by Activating BDNF/TrkB Signaling Pathway

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Titel: Dexmedetomidine Alleviates Hippocampal Tissue Damage in Rapid Eye Movement Sleep-Deprived Rats by Activating BDNF/TrkB Signaling Pathway
Autoren: Zheng B, Li Y, Liu C
Quelle: Nature and Science of Sleep, Vol 17, Iss Issue 1, Pp 2423-2435 (2025)
Verlagsinformationen: Dove Medical Press, 2025.
Publikationsjahr: 2025
Bestand: LCC:Psychiatry
LCC:Neurophysiology and neuropsychology
Schlagwörter: Dexmedetomidine, rapid eye movement sleep deprivation, hippocampus, BDNF, TrkB, neuroprotection, Psychiatry, RC435-571, Neurophysiology and neuropsychology, QP351-495
Beschreibung: Biqiong Zheng,1,2,* Yang Li,3,* Changyi Liu4,5 1Department of Anesthesiology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, People’s Republic of China; 2Department of Anesthesiology, National Regional Medical Center, Binhai Campus of The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350212, People’s Republic of China; 3Department of Anesthesiology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, Fujian Province, 361001, People’s Republic of China; 4Department of Urology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, People’s Republic of China; 5Department of Urology, National Regional Medical Center, Binhai Campus of The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350212, People’s Republic of China*These authors contributed equally to this workCorrespondence: Changyi Liu, Department of Urology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, No. 20, Cha Zhong Road, Fuzhou, Fujian Province, People’s Republic of China, Email lcy900125@fjmu.edu.cnIntroduction: Sleep deprivation often leads to marked neurobehavioral and cognitive deficits, yet few well-defined interventions exist to address these effects. Dexmedetomidine (DEX), a highly selective α2-adrenoceptor agonist, possesses sedative, hypnotic, analgesic, and sympathetic-blocking properties, closely mimic natural sleep state. In this study, we aim to investigate whether DEX protects hippocampal tissue against rapid eye movement sleep deprivation (RSD)-induced injury in rats and to explore the underlying molecular mechanisms.Methods: In this study, a rapid eye movement sleep deprivation (RSD) rat model was created using a modified multi-platform method. The influence of dexmedetomidine (DEX) on hippocampal tissue morphology, the BDNF/TrkB signaling pathway, and cognitive function was then evaluated. Group comparisons were analyzed using one-way ANOVA followed by appropriate post hoc tests.Results: In comparison with the control group, DEX significantly alleviated the impaired spatial learning and memory as reflected escape latency and increased the time spent in the garget quadrant. ANA-12 reversed these improvements, indicating DEX’s cognitive benefits. HE staining showed that DEX protected neurons from RSD-induced injury by preserving structural integrity and TUNEL assay demonstrated reduced neuron apoptosis in the DEX group. Co-treatment with ANA-12 abolished these protective effects, resulting in neuronal damage and apoptosis levels similar to those observed in RSD rats. Moreover, compared with the level of TNA alpha in RSD rats, IL 6, IL 1beta and MDA levels were lower in the hippocampus of DEX group, while SOD activity was enhanced. Western blot analysis revealed that DEX increased hippocampal BDNF (0.586 ± 0.036 vs 0.315 ± 0.034, ~1.86‑fold, P
Publikationsart: article
Dateibeschreibung: electronic resource
Sprache: English
ISSN: 1179-1608
Relation: https://www.dovepress.com/dexmedetomidine-alleviates-hippocampal-tissue-damage-in-rapid-eye-move-peer-reviewed-fulltext-article-NSS; https://doaj.org/toc/1179-1608
Zugangs-URL: https://doaj.org/article/5a2a764bf5fb4f6ca9ce543bf567da9c
Dokumentencode: edsdoj.5a2a764bf5fb4f6ca9ce543bf567da9c
Datenbank: Directory of Open Access Journals
Beschreibung
Abstract:Biqiong Zheng,1,2,* Yang Li,3,* Changyi Liu4,5 1Department of Anesthesiology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, People’s Republic of China; 2Department of Anesthesiology, National Regional Medical Center, Binhai Campus of The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350212, People’s Republic of China; 3Department of Anesthesiology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, Fujian Province, 361001, People’s Republic of China; 4Department of Urology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, People’s Republic of China; 5Department of Urology, National Regional Medical Center, Binhai Campus of The First Affiliated Hospital, Fujian Medical University, Fuzhou, 350212, People’s Republic of China*These authors contributed equally to this workCorrespondence: Changyi Liu, Department of Urology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, No. 20, Cha Zhong Road, Fuzhou, Fujian Province, People’s Republic of China, Email lcy900125@fjmu.edu.cnIntroduction: Sleep deprivation often leads to marked neurobehavioral and cognitive deficits, yet few well-defined interventions exist to address these effects. Dexmedetomidine (DEX), a highly selective α2-adrenoceptor agonist, possesses sedative, hypnotic, analgesic, and sympathetic-blocking properties, closely mimic natural sleep state. In this study, we aim to investigate whether DEX protects hippocampal tissue against rapid eye movement sleep deprivation (RSD)-induced injury in rats and to explore the underlying molecular mechanisms.Methods: In this study, a rapid eye movement sleep deprivation (RSD) rat model was created using a modified multi-platform method. The influence of dexmedetomidine (DEX) on hippocampal tissue morphology, the BDNF/TrkB signaling pathway, and cognitive function was then evaluated. Group comparisons were analyzed using one-way ANOVA followed by appropriate post hoc tests.Results: In comparison with the control group, DEX significantly alleviated the impaired spatial learning and memory as reflected escape latency and increased the time spent in the garget quadrant. ANA-12 reversed these improvements, indicating DEX’s cognitive benefits. HE staining showed that DEX protected neurons from RSD-induced injury by preserving structural integrity and TUNEL assay demonstrated reduced neuron apoptosis in the DEX group. Co-treatment with ANA-12 abolished these protective effects, resulting in neuronal damage and apoptosis levels similar to those observed in RSD rats. Moreover, compared with the level of TNA alpha in RSD rats, IL 6, IL 1beta and MDA levels were lower in the hippocampus of DEX group, while SOD activity was enhanced. Western blot analysis revealed that DEX increased hippocampal BDNF (0.586 ± 0.036 vs 0.315 ± 0.034, ~1.86‑fold, P
ISSN:11791608