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Biological properties and pathogenetic potential of Weeksellaceae family bacteria

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Titel: Biological properties and pathogenetic potential of Weeksellaceae family bacteria
Autoren: Ksenia V. Zubova, Victoria A. Kuznetsova, Matvey V. Kanevsky, Olga V. Kondratenko, Elena V. Glinskaya, Olga V. Nechaeva, Anna G. Afinogenova
Quelle: Инфекция и иммунитет, Vol 15, Iss 3, Pp 505-516 (2025)
Verlagsinformationen: NIIÈM imeni Pastera, 2025.
Sankt-Peterburg , 2025.
Publikationsjahr: 2025
Bestand: LCC:Infectious and parasitic diseases
Schlagwörter: saprophytic bacteria, cystic fibrosis, virulence genes, enzymatic activity of bacteria, weeksellaceae, flavobacteriales, Infectious and parasitic diseases, RC109-216
Beschreibung: In recent years, the role of former saprophyte bacteria in the etiology of human infectious diseases has become an increasingly pressing issue. Of particular interest are non-fermenting gram-negative bacteria, many of which are members of the rhizobiome in some plants, soil and water microbiome. Increasingly, scientific literature covers cases of infections caused by members of the order Flavobacteriales, namely the genus Chryseobacterium, Elizabethkingia and Empedobacter of the Weeksellaceae family. Microorganisms of the latter are causative agents in several human diseases, including urinary tract and respiratory tract infections. The aim of our study was to investigate the biological properties of bacteria of the genus Chryseobacterium, Elizabethkingia and Empedobacter of the Weeksellaceae family isolated from patients with cystic fibrosis and to assess their pathogenetic potential. To analyze the biological properties and pathogenetic potential, 32 clinical strains of bacteria belonging to the Weeksellaceae family, isolated from biological material samples of the respiratory tract from cystic fibrosis patients, were selected for the study. Differential diagnostic media and plate test systems were used. DNA was isolated using 5X ScreenMix (ZAO Evrogen) and PCR with gel electrophoresis was performed. Bacteria of the Weeksellaceae family are characterized by a wide range of enzymes, especially C. arthrosphaerae and E. meningoseptica. It was found that strains of the Chryseobacterium, Elizabethkingia and Empedobacter genus are carriers of virulence genes, exhibit pathogenicity and participate in chronic respiratory infections in patients with cystic fibrosis. Phenotypic methods for studying enzymatic activity showed that all the examined strains of bacteria of the Weeksellaceae family are characterized by gelatinase activity, and representatives of the genus Chryseobacterium spp. — also by the ability to synthesize plasma coagulase. The potential to produce other enzymes of aggression is less pronounced and was strain specific. Analysis of the genetic profile of virulence factors in the studied bacteria showed that in their genome, genes encoding the enzymes lecithinase, hyaluronidase, elastase and hemolysin were most often found, and in bacteria of the genus Chryseobacterium spp. and Empedobacter spp. — neuroaminidase, which contribute to overcoming tissue barriers, pathogen invasion and spread throughout host tissues. The obtained results and scientific literature data indicate a special clinical role for bacteria of the Weeksellaceae family, which determines the importance of a deep study of the biological properties and virulence factors in members of the genus Chryseobacterium spp., Elizabethkingia spp. and Empedobacter spp.
Publikationsart: article
Dateibeschreibung: electronic resource
Sprache: Russian
ISSN: 2220-7619
2313-7398
Relation: https://iimmun.ru/iimm/article/viewFile/17859/2224; https://doaj.org/toc/2220-7619; https://doaj.org/toc/2313-7398
DOI: 10.15789/2220-7619-BPA-17859
Zugangs-URL: https://doaj.org/article/c52aa0c40d24441489fc689ce8af3bb1
Dokumentencode: edsdoj.52aa0c40d24441489fc689ce8af3bb1
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  Data: Biological properties and pathogenetic potential of Weeksellaceae family bacteria
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  Data: <searchLink fieldCode="AR" term="%22Ksenia+V%2E+Zubova%22">Ksenia V. Zubova</searchLink><br /><searchLink fieldCode="AR" term="%22Victoria+A%2E+Kuznetsova%22">Victoria A. Kuznetsova</searchLink><br /><searchLink fieldCode="AR" term="%22Matvey+V%2E+Kanevsky%22">Matvey V. Kanevsky</searchLink><br /><searchLink fieldCode="AR" term="%22Olga+V%2E+Kondratenko%22">Olga V. Kondratenko</searchLink><br /><searchLink fieldCode="AR" term="%22Elena+V%2E+Glinskaya%22">Elena V. Glinskaya</searchLink><br /><searchLink fieldCode="AR" term="%22Olga+V%2E+Nechaeva%22">Olga V. Nechaeva</searchLink><br /><searchLink fieldCode="AR" term="%22Anna+G%2E+Afinogenova%22">Anna G. Afinogenova</searchLink>
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  Data: Инфекция и иммунитет, Vol 15, Iss 3, Pp 505-516 (2025)
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  Data: NIIÈM imeni Pastera, 2025.<br />Sankt-Peterburg , 2025.
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  Data: 2025
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  Data: LCC:Infectious and parasitic diseases
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  Data: <searchLink fieldCode="DE" term="%22saprophytic+bacteria%22">saprophytic bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22cystic+fibrosis%22">cystic fibrosis</searchLink><br /><searchLink fieldCode="DE" term="%22virulence+genes%22">virulence genes</searchLink><br /><searchLink fieldCode="DE" term="%22enzymatic+activity+of+bacteria%22">enzymatic activity of bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22weeksellaceae%22">weeksellaceae</searchLink><br /><searchLink fieldCode="DE" term="%22flavobacteriales%22">flavobacteriales</searchLink><br /><searchLink fieldCode="DE" term="%22Infectious+and+parasitic+diseases%22">Infectious and parasitic diseases</searchLink><br /><searchLink fieldCode="DE" term="%22RC109-216%22">RC109-216</searchLink>
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  Label: Description
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  Data: In recent years, the role of former saprophyte bacteria in the etiology of human infectious diseases has become an increasingly pressing issue. Of particular interest are non-fermenting gram-negative bacteria, many of which are members of the rhizobiome in some plants, soil and water microbiome. Increasingly, scientific literature covers cases of infections caused by members of the order Flavobacteriales, namely the genus Chryseobacterium, Elizabethkingia and Empedobacter of the Weeksellaceae family. Microorganisms of the latter are causative agents in several human diseases, including urinary tract and respiratory tract infections. The aim of our study was to investigate the biological properties of bacteria of the genus Chryseobacterium, Elizabethkingia and Empedobacter of the Weeksellaceae family isolated from patients with cystic fibrosis and to assess their pathogenetic potential. To analyze the biological properties and pathogenetic potential, 32 clinical strains of bacteria belonging to the Weeksellaceae family, isolated from biological material samples of the respiratory tract from cystic fibrosis patients, were selected for the study. Differential diagnostic media and plate test systems were used. DNA was isolated using 5X ScreenMix (ZAO Evrogen) and PCR with gel electrophoresis was performed. Bacteria of the Weeksellaceae family are characterized by a wide range of enzymes, especially C. arthrosphaerae and E. meningoseptica. It was found that strains of the Chryseobacterium, Elizabethkingia and Empedobacter genus are carriers of virulence genes, exhibit pathogenicity and participate in chronic respiratory infections in patients with cystic fibrosis. Phenotypic methods for studying enzymatic activity showed that all the examined strains of bacteria of the Weeksellaceae family are characterized by gelatinase activity, and representatives of the genus Chryseobacterium spp. — also by the ability to synthesize plasma coagulase. The potential to produce other enzymes of aggression is less pronounced and was strain specific. Analysis of the genetic profile of virulence factors in the studied bacteria showed that in their genome, genes encoding the enzymes lecithinase, hyaluronidase, elastase and hemolysin were most often found, and in bacteria of the genus Chryseobacterium spp. and Empedobacter spp. — neuroaminidase, which contribute to overcoming tissue barriers, pathogen invasion and spread throughout host tissues. The obtained results and scientific literature data indicate a special clinical role for bacteria of the Weeksellaceae family, which determines the importance of a deep study of the biological properties and virulence factors in members of the genus Chryseobacterium spp., Elizabethkingia spp. and Empedobacter spp.
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  Data: 2220-7619<br />2313-7398
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  Data: https://iimmun.ru/iimm/article/viewFile/17859/2224; https://doaj.org/toc/2220-7619; https://doaj.org/toc/2313-7398
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  Data: 10.15789/2220-7619-BPA-17859
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        Value: 10.15789/2220-7619-BPA-17859
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      – Text: Russian
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        PageCount: 12
        StartPage: 505
    Subjects:
      – SubjectFull: saprophytic bacteria
        Type: general
      – SubjectFull: cystic fibrosis
        Type: general
      – SubjectFull: virulence genes
        Type: general
      – SubjectFull: enzymatic activity of bacteria
        Type: general
      – SubjectFull: weeksellaceae
        Type: general
      – SubjectFull: flavobacteriales
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