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MSD2, an apoplastic Mn-SOD, contributes to root skotomorphogenic growth by modulating ROS distribution in Arabidopsis.

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Bibliographic Details
Title: MSD2, an apoplastic Mn-SOD, contributes to root skotomorphogenic growth by modulating ROS distribution in Arabidopsis.
Authors: Chen, H., Lee, J., Lee, J.M., Han, M., Emonet, A., Jia, X., Lee, Y.
Publication Year: 2025
Collection: Université de Lausanne (UNIL): Serval - Serveur académique lausannois
Subject Terms: Arabidopsis/enzymology, Arabidopsis/genetics, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Hydrogen Peroxide/metabolism, NADPH Oxidases/genetics, NADPH Oxidases/metabolism, Plant Roots/growth & development, Plant Roots/metabolism, Reactive Oxygen Species/metabolism, Superoxide Dismutase/genetics, Superoxide Dismutase/metabolism, Light response, ROS metabolism, Root growth, Skotomorphogenesis, Superoxide dismutase
Description: Reactive oxygen species (ROS) play essential roles as a second messenger in various physiological processes in plants. Due to their oxidative nature, ROS can also be harmful. Thus, the generation and homeostasis of ROS are tightly controlled by multiple enzymes. Membrane-localized NADPH oxidases are well known to generate ROS during developmental and stress responses, but the metabolic pathways of the superoxide (O2 - ) generated by them in the apoplast are poorly understood, and the identity of the apoplastic superoxide dismutase (SOD) is unknown in Arabidopsis. Here, we show that a putative manganese SOD, MSD2 is secreted and possesses a SOD activity that can be inhibited by nitration at tyrosine 68. The expression of MSD2 in roots is light condition-dependent, suggesting that MSD2 may act on ROS metabolism in roots during the light-to-dark transition. Root architecture is governed by ROS distribution that exhibits opposite gradient of H 2 O 2 and O2 - , which is indeed altered in etiolated msd2 mutants and accompanied by changes in the onset of differentiation. These results provide a missing link in our understanding of ROS metabolism and suggest that MSD2 plays a role in root skotomorphogenesis by regulating ROS distribution, thereby playing a pivotal role in plant growth and development.
Document Type: article in journal/newspaper
Language: English
ISSN: 1873-2259
Relation: Plant Science: An international journal of experimental plant biology; https://iris.unil.ch/handle/iris/248535; serval:BIB_FD3FF5D6CA10; 000747007500005
DOI: 10.1016/j.plantsci.2022.111192
Availability: https://iris.unil.ch/handle/iris/248535
https://doi.org/10.1016/j.plantsci.2022.111192
Accession Number: edsbas.E80A7F76
Database: BASE
Description
Abstract:Reactive oxygen species (ROS) play essential roles as a second messenger in various physiological processes in plants. Due to their oxidative nature, ROS can also be harmful. Thus, the generation and homeostasis of ROS are tightly controlled by multiple enzymes. Membrane-localized NADPH oxidases are well known to generate ROS during developmental and stress responses, but the metabolic pathways of the superoxide (O2 - ) generated by them in the apoplast are poorly understood, and the identity of the apoplastic superoxide dismutase (SOD) is unknown in Arabidopsis. Here, we show that a putative manganese SOD, MSD2 is secreted and possesses a SOD activity that can be inhibited by nitration at tyrosine 68. The expression of MSD2 in roots is light condition-dependent, suggesting that MSD2 may act on ROS metabolism in roots during the light-to-dark transition. Root architecture is governed by ROS distribution that exhibits opposite gradient of H 2 O 2 and O2 - , which is indeed altered in etiolated msd2 mutants and accompanied by changes in the onset of differentiation. These results provide a missing link in our understanding of ROS metabolism and suggest that MSD2 plays a role in root skotomorphogenesis by regulating ROS distribution, thereby playing a pivotal role in plant growth and development.
ISSN:18732259
DOI:10.1016/j.plantsci.2022.111192