Disseminating metaproteomic informatics capabilities and knowledge using the Galaxy-P framework
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| Titel: | Disseminating metaproteomic informatics capabilities and knowledge using the Galaxy-P framework |
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| Autoren: | Blank, Clemens, Easterly, Caleb, Gruening, Bjoern, Johnson, James, Kolmeder, Carolin A, Kumar, Praveen, May, Damon, Mehta, Subina, Mesuere, Bart, Brown, Zachary, Elias, Joshua E, Hervey, W Judson, McGowan, Thomas, Muth, Thilo, Nunn, Brook L, Rudney, Joel, Tanca, Alessandro, Griffin, Timothy J, Jagtap, Pratik D |
| Quelle: | PROTEOMES ; ISSN: 2227-7382 |
| Publikationsjahr: | 2018 |
| Bestand: | Ghent University Academic Bibliography |
| Schlagwörter: | Biology and Life Sciences, metaproteomics, functional microbiome, bioinformatics, software workflow development, Galaxy platform, mass spectrometry, community development, MICROBIAL COMMUNITIES, GUT MICROBIOTA, IDENTIFICATION, FRACTIONATION, LOCALIZATION, ANNOTATIONS, PROTEOMICS, PROTEINS, PIPELINE, SAMPLES |
| Beschreibung: | The impact of microbial communities, also known as the microbiome, on human health and the environment is receiving increased attention. Studying translated gene products (proteins) and comparing metaproteomic profiles may elucidate how microbiomes respond to specific environmental stimuli, and interact with host organisms. Characterizing proteins expressed by a complex microbiome and interpreting their functional signature requires sophisticated informatics tools and workflows tailored to metaproteomics. Additionally, there is a need to disseminate these informatics resources to researchers undertaking metaproteomic studies, who could use them to make new and important discoveries in microbiome research. The Galaxy for proteomics platform (Galaxy-P) offers an open source, web-based bioinformatics platform for disseminating metaproteomics software and workflows. Within this platform, we have developed easily-accessible and documented metaproteomic software tools and workflows aimed at training researchers in their operation and disseminating the tools for more widespread use. The modular workflows encompass the core requirements of metaproteomic informatics: (a) database generation; (b) peptide spectral matching; (c) taxonomic analysis and (d) functional analysis. Much of the software available via the Galaxy-P platform was selected, packaged and deployed through an online metaproteomics Contribution Fest undertaken by a unique consortium of expert software developers and users from the metaproteomics research community, who have co-authored this manuscript. These resources are documented on GitHub and freely available through the Galaxy Toolshed, as well as a publicly accessible metaproteomics gateway Galaxy instance. These documented workflows are well suited for the training of novice metaproteomics researchers, through online resources such as the Galaxy Training Network, as well as hands-on training workshops. Here, we describe the metaproteomics tools available within these Galaxy-based resources, as well ... |
| Publikationsart: | article in journal/newspaper |
| Dateibeschreibung: | application/pdf |
| Sprache: | English |
| Relation: | https://biblio.ugent.be/publication/8548277; https://doi.org/10.3390/proteomes6010007; https://biblio.ugent.be/publication/8548277/file/8559850 |
| DOI: | 10.3390/proteomes6010007 |
| Verfügbarkeit: | https://biblio.ugent.be/publication/8548277 https://hdl.handle.net/1854/LU-8548277 https://doi.org/10.3390/proteomes6010007 https://biblio.ugent.be/publication/8548277/file/8559850 |
| Rights: | info:eu-repo/semantics/openAccess |
| Dokumentencode: | edsbas.D1D1D6D1 |
| Datenbank: | BASE |
Nájsť tento článok vo Web of Science | Abstract: | The impact of microbial communities, also known as the microbiome, on human health and the environment is receiving increased attention. Studying translated gene products (proteins) and comparing metaproteomic profiles may elucidate how microbiomes respond to specific environmental stimuli, and interact with host organisms. Characterizing proteins expressed by a complex microbiome and interpreting their functional signature requires sophisticated informatics tools and workflows tailored to metaproteomics. Additionally, there is a need to disseminate these informatics resources to researchers undertaking metaproteomic studies, who could use them to make new and important discoveries in microbiome research. The Galaxy for proteomics platform (Galaxy-P) offers an open source, web-based bioinformatics platform for disseminating metaproteomics software and workflows. Within this platform, we have developed easily-accessible and documented metaproteomic software tools and workflows aimed at training researchers in their operation and disseminating the tools for more widespread use. The modular workflows encompass the core requirements of metaproteomic informatics: (a) database generation; (b) peptide spectral matching; (c) taxonomic analysis and (d) functional analysis. Much of the software available via the Galaxy-P platform was selected, packaged and deployed through an online metaproteomics Contribution Fest undertaken by a unique consortium of expert software developers and users from the metaproteomics research community, who have co-authored this manuscript. These resources are documented on GitHub and freely available through the Galaxy Toolshed, as well as a publicly accessible metaproteomics gateway Galaxy instance. These documented workflows are well suited for the training of novice metaproteomics researchers, through online resources such as the Galaxy Training Network, as well as hands-on training workshops. Here, we describe the metaproteomics tools available within these Galaxy-based resources, as well ... |
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| DOI: | 10.3390/proteomes6010007 |