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Polysaccharide fucoidan BAM1 and arabinogalactan-protein glycan JIM13 antibody binding in sediment cores from coastal vegetated ecosystems

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Název: Polysaccharide fucoidan BAM1 and arabinogalactan-protein glycan JIM13 antibody binding in sediment cores from coastal vegetated ecosystems
Autoři: Hellige, Inga, Akeerath Mundanatt, Aman, Fett, Theresa-Marie, Wölfelschneider, Mirco, Prugger, Jennifer, Geuer, Jana K, Reents, Svenja
Informace o vydavateli: PANGAEA
Rok vydání: 2025
Sbírka: PANGAEA - Data Publisher for Earth & Environmental Science (AWI Bremerhaven / MARUM Bremen)
Témata: Arabinogalactan protein glycan, relative JIM13 antibody signal, carbon dioxide removal, CDRmare, coastal vegetated ecosystems, Colombia, Comment, Country, DAM CDRmare - sea4soCiety: Searching for solutions for Carbon-sequestration in coastal ecosystems, DATE/TIME, DEPTH, sediment/rock, bottom/maximum, top/minimum, Depth comment, Ecosystem, Enzyme-linked immunosorbent assay (ELISA) according to Cornuault et al. (2014), Event label, GER_HAH_SG_P4_SC, GER_HAH_SG_P6_SC, GER_HAH_SG_P8_SC, GER_HAH_SM_P2_SC, GER_HAH_SM_P5_SC, GER_HAH_SM_P7_SC, GER_HAH_UV_P4_SC, GER_HAH_UV_P5_SC, GER_HAH_UV_P6_SC, GER_HEI_SG_P3_SC, GER_HEI_SG_P6_SC, GER_HEI_SG_P8_SC
Geografické téma: MEDIAN LATITUDE: 32.411781 * MEDIAN LONGITUDE: 13.507392 * SOUTH-BOUND LATITUDE: 4.660548 * WEST-BOUND LONGITUDE: -75.682996 * NORTH-BOUND LATITUDE: 54.692271 * EAST-BOUND LONGITUDE: 103.439555 * DATE/TIME START: 2021-11-08T00:00:00 * DATE/TIME END: 2022-10-11T00:00:00 * MINIMUM DEPTH, sediment/rock: 0.007 m * MAXIMUM DEPTH, sediment/rock: 0.485 m
Popis: 50-cm deep sediment cores were taken in saltmarsh, seagrass, mangroves and unvegetated areas around the German Bight, Malaysia and Columbia in 2022 and 2023. Up to 3 points per ecosystem were sampled along a transect, in total 93 cores were analysed. Carbohydrates were sequentially extracted using MilliQ-water and 0.3 M EDTA for later analyses. For more specific analysis enzyme-linked immunosorbent assay (ELISA) was used for detection of fucoidan and arabinogalactan-protein glycan as described in the following studies (Vidal-Melgosa et al., 2021 and Cornuault et al., 2014). In short, 100 µL of sediment extracts were added to a pre-coated 96-well plate and incubated overnight at 4°C. The signal was developed using primary antibodies, BAM1 (fucoidan) and JIM13 (arabinogalactan-protein glycan), diluted 1:10 in skim milk PBS solution, followed by anti-rat antibody at a 1:1000 dilution in the same solution. Absorbance was measured at 450 nm using a Spectramax Id3 plate reader (Molecular Devices).
Druh dokumentu: dataset
Popis souboru: text/tab-separated-values, 3060 data points
Jazyk: English
Relation: Akeerath Mundanatt, Aman; Massing, Jana Chiara; Hehemann, Jan-Hendrik; Hellige, Inga (in prep.): Roots of coastal plants stabilize carbon fixed by marine algae.; Cornuault, Valérie; Manfield, Iain W; Ralet, Marie-Christine; Knox, J Paul (2014): Epitope detection chromatography: a method to dissect the structural heterogeneity and inter-connections of plant cell-wall matrix glycans. Plant Journal, 78(4), 715-722, https://doi.org/10.1111/tpj.12504; Vidal-Melgosa, Silvia; Sichert, Andreas; Francis, T Ben; Bartosik, Daniel; Niggemann, Jutta; Wichels, Antje; Willats, William G T; Fuchs, Bernhard M; Teeling, Hanno; Becher, Dörte; Schweder, Thomas; Amann, Rudolf; Hehemann, Jan-Hendrik (2021): Diatom fucan polysaccharide precipitates carbon during algal blooms. Nature Communications, 12(1), https://doi.org/10.1038/s41467-021-21009-6; https://doi.pangaea.de/10.1594/PANGAEA.979912; https://doi.org/10.1594/PANGAEA.979912
DOI: 10.1594/PANGAEA.979912
Dostupnost: https://doi.pangaea.de/10.1594/PANGAEA.979912
https://doi.org/10.1594/PANGAEA.979912
Rights: CC-BY-4.0: Creative Commons Attribution 4.0 International (License comes into effect after moratorium ends) ; Access constraints: access rights needed ; info:eu-repo/semantics/restrictedAccess
Přístupové číslo: edsbas.BFBFB84A
Databáze: BASE
Popis
Abstrakt:50-cm deep sediment cores were taken in saltmarsh, seagrass, mangroves and unvegetated areas around the German Bight, Malaysia and Columbia in 2022 and 2023. Up to 3 points per ecosystem were sampled along a transect, in total 93 cores were analysed. Carbohydrates were sequentially extracted using MilliQ-water and 0.3 M EDTA for later analyses. For more specific analysis enzyme-linked immunosorbent assay (ELISA) was used for detection of fucoidan and arabinogalactan-protein glycan as described in the following studies (Vidal-Melgosa et al., 2021 and Cornuault et al., 2014). In short, 100 µL of sediment extracts were added to a pre-coated 96-well plate and incubated overnight at 4°C. The signal was developed using primary antibodies, BAM1 (fucoidan) and JIM13 (arabinogalactan-protein glycan), diluted 1:10 in skim milk PBS solution, followed by anti-rat antibody at a 1:1000 dilution in the same solution. Absorbance was measured at 450 nm using a Spectramax Id3 plate reader (Molecular Devices).
DOI:10.1594/PANGAEA.979912