Choline Phosphate Surface-Activated 3D-Printed Porous Titanium Scaffold Combined with Stem Cell Exosomes for Enhancing Bone Defects Repair
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| Titel: | Choline Phosphate Surface-Activated 3D-Printed Porous Titanium Scaffold Combined with Stem Cell Exosomes for Enhancing Bone Defects Repair |
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| Autoren: | Xuezhong Cui, Jing Li, Yuemin Wang, Tong Sun, Jie Weng, Zhiqiang Li, Jianshu Li, Xingyu Chen |
| Publikationsjahr: | 2025 |
| Schlagwörter: | Biochemistry, Medicine, Cell Biology, Physiology, Biotechnology, Developmental Biology, Cancer, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, significant therapeutic implications, rabbit femoral defects, modified bioactive coating, microcomputed tomography indicate, hard tissue sectioning, 2 -( methacryloyloxy, ti scaffold enhances, based ti scaffold, ethyl choline phosphate, exosome bioactive coating, enhance osteogenic activity, choline phosphate surface, stem cell exosomes, choline phosphate, osteogenic activity, zwitterionic poly, vivo <, vitro <, utilized surface |
| Beschreibung: | In recent decades, porous titanium (Ti) bone-engineered scaffolds have emerged as a promising biomaterial for bone defect repair due to their excellent biocompatibility and mechanical properties. However, the limited bioactivity on the surface of the porous scaffold hinders osteogenesis and osseointegration, thereby restricting its further application. In this study, we utilized surface-initiated atom transfer radical polymerization to prepare a zwitterionic poly[2-(methacryloyloxy)ethyl choline phosphate] (PMCP) modified bioactive coating on the surface of a 3D-printed porous Ti scaffold. Additionally, exosomes derived from bone mesenchymal stem cells (BMSCs) were introduced into the scaffold surface via specific interactions between choline phosphate and phosphatidylcholine (CP-PC) on exosomes. In vitro studies for ossification and transcriptome analysis have shown that the exosome bioactive coating on a Ti scaffold enhances the proliferation of BMSCs, their osteogenic activity, and the expression of osteogenic-related genes. Furthermore, in vivo study results from hard tissue sectioning and microcomputed tomography indicate that the bioactive Ti scaffold significantly promotes new bone formation after 4 and 12 weeks of implantation in rabbit femoral defects. Overall, this study showcases the potential of the exosome-based Ti scaffold to enhance osteogenic activity, offering a novel strategy for cell-free bone tissue regeneration with significant therapeutic implications. |
| Publikationsart: | article in journal/newspaper |
| Sprache: | unknown |
| DOI: | 10.1021/acsami.5c00953.s001 |
| Verfügbarkeit: | https://doi.org/10.1021/acsami.5c00953.s001 https://figshare.com/articles/journal_contribution/Choline_Phosphate_Surface-Activated_3D-Printed_Porous_Titanium_Scaffold_Combined_with_Stem_Cell_Exosomes_for_Enhancing_Bone_Defects_Repair/28903715 |
| Rights: | CC BY-NC 4.0 |
| Dokumentencode: | edsbas.ADE7641D |
| Datenbank: | BASE |
Nájsť tento článok vo Web of Science | Abstract: | In recent decades, porous titanium (Ti) bone-engineered scaffolds have emerged as a promising biomaterial for bone defect repair due to their excellent biocompatibility and mechanical properties. However, the limited bioactivity on the surface of the porous scaffold hinders osteogenesis and osseointegration, thereby restricting its further application. In this study, we utilized surface-initiated atom transfer radical polymerization to prepare a zwitterionic poly[2-(methacryloyloxy)ethyl choline phosphate] (PMCP) modified bioactive coating on the surface of a 3D-printed porous Ti scaffold. Additionally, exosomes derived from bone mesenchymal stem cells (BMSCs) were introduced into the scaffold surface via specific interactions between choline phosphate and phosphatidylcholine (CP-PC) on exosomes. In vitro studies for ossification and transcriptome analysis have shown that the exosome bioactive coating on a Ti scaffold enhances the proliferation of BMSCs, their osteogenic activity, and the expression of osteogenic-related genes. Furthermore, in vivo study results from hard tissue sectioning and microcomputed tomography indicate that the bioactive Ti scaffold significantly promotes new bone formation after 4 and 12 weeks of implantation in rabbit femoral defects. Overall, this study showcases the potential of the exosome-based Ti scaffold to enhance osteogenic activity, offering a novel strategy for cell-free bone tissue regeneration with significant therapeutic implications. |
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| DOI: | 10.1021/acsami.5c00953.s001 |