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Experimental test of ensemble inequivalence and the fluctuation theorem in the force ensemble in DNA pulling experiments

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Bibliographic Details
Title: Experimental test of ensemble inequivalence and the fluctuation theorem in the force ensemble in DNA pulling experiments
Authors: Martínez Monge, Álvaro, Mañosas Castejón, María, Ritort Farran, Fèlix
Source: Articles publicats en revistes (Física de la Matèria Condensada)
Publisher Information: American Physical Society
Publication Year: 2018
Collection: Dipòsit Digital de la Universitat de Barcelona
Subject Terms: Aparells i instruments científics, Dispositius optoelectrònics, Mecànica estadística, Scientific apparatus and instruments, Optoelectronic devices, Statistical mechanics
Description: We experimentally test the validity of the Crooks fluctuation theorem (CFT) in the force ensemble by pulling DNA hairpins, first with magnetic tweezers, next with optical tweezers using force feedback. The CFT holds when using the definition of work Wf=−∫xdf, where x is the molecular extension and f is the force. In contrast, it does not hold when using the usual definition, appropriate for the constant extension ensemble, Wx=∫fdx, showing the importance of the contribution of boundary terms to the full entropy production in a clear example of statistical ensemble inequivalence in small systems. We also evaluate the differences in the average dissipated work in the force ensemble as compared to the extension ensemble, highlighting ensemble inequivalence also at the level of molecular kinetics.
Document Type: article in journal/newspaper
File Description: 9 p.; application/pdf
Language: English
Relation: Reproducció del document publicat a: https://doi.org/10.1103/PhysRevE.98.032146; Physical Review E, 2018, vol. 98, num. 3; https://doi.org/10.1103/PhysRevE.98.032146; info:eu-repo/grantAgreement/EC/H2020/687089/EU//PROSEQO; https://hdl.handle.net/2445/125334; 682359
Availability: https://hdl.handle.net/2445/125334
Rights: (c) American Physical Society, 2018 ; info:eu-repo/semantics/openAccess
Accession Number: edsbas.9D4B5E16
Database: BASE
Description
Abstract:We experimentally test the validity of the Crooks fluctuation theorem (CFT) in the force ensemble by pulling DNA hairpins, first with magnetic tweezers, next with optical tweezers using force feedback. The CFT holds when using the definition of work Wf=−∫xdf, where x is the molecular extension and f is the force. In contrast, it does not hold when using the usual definition, appropriate for the constant extension ensemble, Wx=∫fdx, showing the importance of the contribution of boundary terms to the full entropy production in a clear example of statistical ensemble inequivalence in small systems. We also evaluate the differences in the average dissipated work in the force ensemble as compared to the extension ensemble, highlighting ensemble inequivalence also at the level of molecular kinetics.