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Gene redundancy and gene compensation of insulin-like peptides in the oocyte development of bean beetle

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Název: Gene redundancy and gene compensation of insulin-like peptides in the oocyte development of bean beetle
Autoři: Li, Yongqin, Fang, Zheng, Tan, Leitao, Wu, Qingshan, Liu, Qiuping, Wang, Yeying, Weng, Qingbei, Chen, Qianquan
Přispěvatelé: Hull, J. Joe, National Natural Science Foundation of China, Guizhou Normal University, Guizhou Provincial Science and Technology Foundation, the Joint Fund of the National Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province, Provincial Program on Platform and Talent Development of the Department of Science and Technology of Guizhou China
Zdroj: PLOS ONE ; volume 19, issue 5, page e0302992 ; ISSN 1932-6203
Informace o vydavateli: Public Library of Science (PLoS)
Rok vydání: 2024
Sbírka: PLOS Publications (via CrossRef)
Popis: Bean beetle ( Callosobruchus maculatus ) exhibits clear phenotypic plasticity depending on population density; However, the underlying molecular mechanism remains unknown. Compared to low-density individuals, high-density individuals showed a faster terminal oocyte maturity rate. Four insulin-like peptide (ILP) genes were identified in the bean beetle, which had higher expression levels in the head than in the thorax and abdomen. The population density could regulate the expression levels of CmILP1-3 , CmILP2-3 , and CmILP1 as well as CmILP3 in the head, thorax, and abdomen, respectively. RNA interference results showed that each CmILP could regulate terminal oocyte maturity rate, indicating that there was functional redundancy among CmILPs . Silencing each CmILP could lead to down-regulation of some other CmILPs , however, CmILP3 was up-regulated in the abdomen after silencing CmILP1 or CmILP2 . Compared to single gene silencing, silencing CmILP3 with CmILP1 or CmILP2 at the same time led to more serious retardation in oocyte development, suggesting CmILP3 could be up-regulated to functionally compensate for the down-regulation of CmILP1 and CmILP2 . In conclusion, population density-dependent plasticity in terminal oocyte maturity rate of bean beetle was regulated by CmILPs , which exhibited gene redundancy and gene compensation.
Druh dokumentu: article in journal/newspaper
Jazyk: English
DOI: 10.1371/journal.pone.0302992
Dostupnost: https://doi.org/10.1371/journal.pone.0302992
https://dx.plos.org/10.1371/journal.pone.0302992
Rights: http://creativecommons.org/licenses/by/4.0/
Přístupové číslo: edsbas.5E984CEB
Databáze: BASE
Popis
Abstrakt:Bean beetle ( Callosobruchus maculatus ) exhibits clear phenotypic plasticity depending on population density; However, the underlying molecular mechanism remains unknown. Compared to low-density individuals, high-density individuals showed a faster terminal oocyte maturity rate. Four insulin-like peptide (ILP) genes were identified in the bean beetle, which had higher expression levels in the head than in the thorax and abdomen. The population density could regulate the expression levels of CmILP1-3 , CmILP2-3 , and CmILP1 as well as CmILP3 in the head, thorax, and abdomen, respectively. RNA interference results showed that each CmILP could regulate terminal oocyte maturity rate, indicating that there was functional redundancy among CmILPs . Silencing each CmILP could lead to down-regulation of some other CmILPs , however, CmILP3 was up-regulated in the abdomen after silencing CmILP1 or CmILP2 . Compared to single gene silencing, silencing CmILP3 with CmILP1 or CmILP2 at the same time led to more serious retardation in oocyte development, suggesting CmILP3 could be up-regulated to functionally compensate for the down-regulation of CmILP1 and CmILP2 . In conclusion, population density-dependent plasticity in terminal oocyte maturity rate of bean beetle was regulated by CmILPs , which exhibited gene redundancy and gene compensation.
DOI:10.1371/journal.pone.0302992