Banded dye murex, Hexaplex trunculus measurements of total weight, soft tissue weight and net calcification rate in males and females over the 310 day ocean acidification experiment
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| Title: | Banded dye murex, Hexaplex trunculus measurements of total weight, soft tissue weight and net calcification rate in males and females over the 310 day ocean acidification experiment |
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| Authors: | Grđan, Sanja, Dupont, Sam, Glamuzina, Luka, Cetinić, Ana Bratoš |
| Publisher Information: | PANGAEA |
| Publication Year: | 2025 |
| Collection: | PANGAEA - Data Publisher for Earth & Environmental Science (AWI Bremerhaven / MARUM Bremen) |
| Subject Terms: | Alkalinity, total, standard deviation, Animalia, Aragonite saturation state, Bay_Bistrina, Benthic animals, Benthos, Bicarbonate ion, Calcification/Dissolution, Calcification rate, Calcite saturation state, Calculated using CO2SYS, Calculated using seacarb after Nisumaa et al. (2010), Calculated using seacarb after Orr et al. (2018), Carbon, inorganic, dissolved, Carbonate ion, Carbonate system computation flag, Carbon dioxide, Coast and continental shelf, Containers and aquaria (20-1000 L or < 1 m**2), EXP, Experiment, Fugacity of carbon dioxide (water) at sea surface temperature (wet air), Fugacity of carbon dioxide in seawater, Growth/Morphology, Growth rate, Hexaplex trunculus |
| Subject Geographic: | LATITUDE: 42.871900 * LONGITUDE: 17.700600 * DATE/TIME START: 2020-08-01T00:00:00 * DATE/TIME END: 2020-08-31T00:00:00 |
| Description: | Physiological traits of banded dye murex were measured during the exposure to a range of pH over the course of 310 days. Gastropods were collected in Bistrina Bay, part of the Mali Ston Bay in the Southeastern Adriatic Sea (42°52'19.1 N 17°42'02.3 E). Experiment was performed in Laboratory for Mariculture, University of Dubrovnik. Banded dye murexes were maintained in nine treatment tanks (volume 130L) with a flow-through water system with filtered, UV-sterilized, and aerated ambient seawater pumped directly from the Bistrina Bay adjacent to the laboratory facilities. Nine pH treatments were assigned to tanks, ranging from pHT 7.95 to 7.22. pH was manipulated by bubling pure CO2 gas, controlled with pH computers (Milwaukee MC122).Temperature, salinity, dissolved oxygen concentration were measured using multiparametric probes (YSI Pro 30 & Oxygen Handy Polaris, respectively). pH was measured on a total scale (pHT) by the potentiometric method with TRIS (2-amino-2-hydroxy-1,3-propanediol) buffer. Total alkalinity (TA, µmol kg-1) was determined with the potentiometric two-point open-cell 0.1 M HCl titration. Other seawater carbonate chemistry parameters (pCO2, ΩCa, ΩAr) were calculated based on known TA and pHT for a given salinity using CO2SYS software. Measurments of total weight were performed eight times during the experiment: day 28, 59, 91, 133, 172, 196, 236 & 310. Total wet weight was measured to the nearest 0.01 g on the top-load scale (Mettler toledo JL602-G/L) for the same individuals nine times over the course of experiment. Prior to weighing, snails were placed on absorbent blotting paper for approximately 20 minutes to remove excess water. The procedure was repeated eight times during the experiment (day 28, 59, 91, 133, 172, 196, 236, 310). Total weight growth rate (TWGR in g day-1) was calculated for each individual as the change in the shell weight between successive observation points divided by time. Net calcification rate was measured with a buoyant weight technique where the weight of ... |
| Document Type: | dataset |
| File Description: | text/tab-separated-values, 20068 data points |
| Language: | English |
| Relation: | Grđan, Sanja; Dupont, Sam; Glamuzina, Luka; Cetinić, Ana Bratoš (2025): When time reveals the cost: effects of long-term exposure to low pH on a predatory gastropod. Marine Ecology, 46, e70039, https://doi.org/10.1111/maec.70039; Grđan, Sanja; Dupont, Sam; Glamuzina, Luka; Bratoš Cetinić, Ana (2024): Seawater carbonate chemistry and shell growth rate of Hexaplex trunculus, an important predatory gastropod in benthic communities of Mali Ston Bay [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.965891; Dickson, Andrew G (1993): pH buffers for sea water media based on the total hydrogen ion concentration scale. Deep Sea Research Part I: Oceanographic Research Papers, 40(1), 107-118, https://doi.org/10.1016/0967-0637(93)90055-8; Dickson, Andrew G; Millero, Frank J (1987): A comparison of the equilibrium constants for the dissociation of carbonic acid in seawater media. Deep Sea Research Part A. Oceanographic Research Papers, 34(10), 1733-1743, https://doi.org/10.1016/0198-0149(87)90021-5; Dickson, Andrew G; Sabine, Christopher L; Christian, J R (2007): Guide to Best Practices for Ocean CO2 Measurements. PICES Special Publication, 3, 191 pp, hdl:10013/epic.51789.d001; Grđan, Sanja; Dupont, Sam; Glamuzina, Luka; Bratoš Cetinić, Ana (2023): Potential for acclimation of banded-dye murex, Hexaplex trunculus (Linnaeus, 1758) after long-term exposure to low pH. Naše more, 70(3), 137-146, https://doi.org/10.17818/NM/2023/SI1; Lewis, Ernie R; Wallace, Douglas WR (1998): Program Developed for CO2 System Calculations. Environmental System Science Data Infrastructure for a Virtual Ecosystem, https://doi.org/10.15485/1464255; Nisumaa, Anne-Marin; Pesant, Stephane; Bellerby, Richard G J; Delille, Bruno; Middelburg, Jack J; Orr, James C; Riebesell, Ulf; Tyrrell, Toby; Wolf-Gladrow, Dieter A; Gattuso, Jean-Pierre (2010): EPOCA/EUR-OCEANS data compilation on the biological and biogeochemical responses to ocean acidification. Earth System Science Data, 2(2), 167-175, https://doi.org/10.5194/essd-2-167-2010; Palmer, A R (1982): Growth in marine gastropods: A non-destructive technique for independently measuring shell and body weight. Malacologia, 23, 63-67; Yang, Yan; Brockmann, Patrick; Galdino, Carolina; Schindler, Uwe; Gazeau, Frédéric (2024): An update of data compilation on the biological response to ocean acidification and overview of the OA-ICC data portal. Earth System Science Data, 16(8), 3771-3780, https://doi.org/10.5194/essd-16-3771-2024; Yang, Yan; Hansson, L; Gattuso, Jean-Pierre (2016): Data compilation on the biological response to ocean acidification: an update. Earth System Science Data, 8(1), 79-87, https://doi.org/10.5194/essd-8-79-2016; Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2024): seacarb: seawater carbonate chemistry with R. R package version 3.3.3. https://cran.r-project.org/web/packages/seacarb/index.html; Carbonate chemistry (URI: https://download.pangaea.de/reference/132613/attachments/Carbonate%20chemistry.xlsx); Data that are going to be presented in figures in a paper (URI: https://download.pangaea.de/reference/132614/attachments/Htrunculus_PHYSdata_updated.xlsx); https://doi.pangaea.de/10.1594/PANGAEA.980753; https://doi.org/10.1594/PANGAEA.980753 |
| DOI: | 10.1594/PANGAEA.980753 |
| Availability: | https://doi.pangaea.de/10.1594/PANGAEA.980753 https://doi.org/10.1594/PANGAEA.980753 |
| Rights: | CC-BY-4.0: Creative Commons Attribution 4.0 International ; Access constraints: unrestricted ; info:eu-repo/semantics/openAccess |
| Accession Number: | edsbas.5912B889 |
| Database: | BASE |
Nájsť tento článok vo Web of Science | Abstract: | Physiological traits of banded dye murex were measured during the exposure to a range of pH over the course of 310 days. Gastropods were collected in Bistrina Bay, part of the Mali Ston Bay in the Southeastern Adriatic Sea (42°52'19.1 N 17°42'02.3 E). Experiment was performed in Laboratory for Mariculture, University of Dubrovnik. Banded dye murexes were maintained in nine treatment tanks (volume 130L) with a flow-through water system with filtered, UV-sterilized, and aerated ambient seawater pumped directly from the Bistrina Bay adjacent to the laboratory facilities. Nine pH treatments were assigned to tanks, ranging from pHT 7.95 to 7.22. pH was manipulated by bubling pure CO2 gas, controlled with pH computers (Milwaukee MC122).Temperature, salinity, dissolved oxygen concentration were measured using multiparametric probes (YSI Pro 30 & Oxygen Handy Polaris, respectively). pH was measured on a total scale (pHT) by the potentiometric method with TRIS (2-amino-2-hydroxy-1,3-propanediol) buffer. Total alkalinity (TA, µmol kg-1) was determined with the potentiometric two-point open-cell 0.1 M HCl titration. Other seawater carbonate chemistry parameters (pCO2, ΩCa, ΩAr) were calculated based on known TA and pHT for a given salinity using CO2SYS software. Measurments of total weight were performed eight times during the experiment: day 28, 59, 91, 133, 172, 196, 236 & 310. Total wet weight was measured to the nearest 0.01 g on the top-load scale (Mettler toledo JL602-G/L) for the same individuals nine times over the course of experiment. Prior to weighing, snails were placed on absorbent blotting paper for approximately 20 minutes to remove excess water. The procedure was repeated eight times during the experiment (day 28, 59, 91, 133, 172, 196, 236, 310). Total weight growth rate (TWGR in g day-1) was calculated for each individual as the change in the shell weight between successive observation points divided by time. Net calcification rate was measured with a buoyant weight technique where the weight of ... |
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| DOI: | 10.1594/PANGAEA.980753 |