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Protein profiling of human plasma samples by two-dimensional electrophoresis.

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Title: Protein profiling of human plasma samples by two-dimensional electrophoresis.
Contributors: Sang Yun Cho, Eun-Young Lee, Hye-Young Kim, Min-Jung Kang, Hyoung-Joo Lee, Hoguen Kim, Young-Ki Paik, Kim, Ho Keun
Publication Year: 2008
Subject Terms: Biomarkers, Tumor/blood, Blood Protein Electrophoresis/methods, Blood Proteins/isolation & purification, Carcinoma, Hepatocellular/blood, Chromatography, Affinity, High Pressure Liquid, Electrophoresis, Gel, Two-Dimensional/methods, Humans, Isoelectric Focusing, Liver Neoplasms/blood, Peptide Mapping, Protein Array Analysis/methods, Proteome/isolation & purification, Proteomics/methods, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Staining and Labeling
Description: Human plasma is regarded the most complex and well-known clinical specimen that can be easily obtained; alterations in the levels of plasma proteins or their corresponding enzyme activities may reflect either a healthy or a diseased state. Given that there is no defined genomic information as to the intact protein components in plasma, protein profiling could be the first step toward its molecular characterization. Several problems exist in the analysis of plasma proteins, however. For example, the widest dynamic range of protein concentrations, the presence of high-abundance proteins, and post-translational modifications need to be considered before proteomic studies are undertaken. In particular, efficient depletion or pre-fractionation of high-abundance proteins is crucial for the identification of low-abundance proteins that may contain potential biomarkers. After the removal of high-abundance proteins, protein profiling can be initiated using two-dimensional electrophoresis (2DE), which has been widely used for displaying the differential proteome under specific physiological conditions. Here, we describe a typical 2DE procedure for plasma proteome under either a healthy or a diseased state (e.g., liver cancer) in which pre-fractionation and depletion are integral steps in the search for disease biomarkers. ; open
Document Type: article in journal/newspaper
File Description: 57~75
Language: unknown
Relation: Methods in Molecular Biology (Clifton, N.J.); J02226; https://ir.ymlib.yonsei.ac.kr/handle/22282913/107578; T200801286
Availability: https://ir.ymlib.yonsei.ac.kr/handle/22282913/107578
http://link.springer.com/protocol/10.1007%2F978-1-59745-117-8_4
Rights: CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ ; not free
Accession Number: edsbas.2213C98F
Database: BASE
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  – Url: https://ir.ymlib.yonsei.ac.kr/handle/22282913/107578#
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PubType: Academic Journal
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Protein profiling of human plasma samples by two-dimensional electrophoresis.
– Name: Author
  Label: Contributors
  Group: Au
  Data: Sang Yun Cho<br />Eun-Young Lee<br />Hye-Young Kim<br />Min-Jung Kang<br />Hyoung-Joo Lee<br />Hoguen Kim<br />Young-Ki Paik<br />Kim, Ho Keun
– Name: DatePubCY
  Label: Publication Year
  Group: Date
  Data: 2008
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Biomarkers%22">Biomarkers</searchLink><br /><searchLink fieldCode="DE" term="%22Tumor%2Fblood%22">Tumor/blood</searchLink><br /><searchLink fieldCode="DE" term="%22Blood+Protein+Electrophoresis%2Fmethods%22">Blood Protein Electrophoresis/methods</searchLink><br /><searchLink fieldCode="DE" term="%22Blood+Proteins%2Fisolation+%26+purification%22">Blood Proteins/isolation & purification</searchLink><br /><searchLink fieldCode="DE" term="%22Carcinoma%22">Carcinoma</searchLink><br /><searchLink fieldCode="DE" term="%22Hepatocellular%2Fblood%22">Hepatocellular/blood</searchLink><br /><searchLink fieldCode="DE" term="%22Chromatography%22">Chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Affinity%22">Affinity</searchLink><br /><searchLink fieldCode="DE" term="%22High+Pressure+Liquid%22">High Pressure Liquid</searchLink><br /><searchLink fieldCode="DE" term="%22Electrophoresis%22">Electrophoresis</searchLink><br /><searchLink fieldCode="DE" term="%22Gel%22">Gel</searchLink><br /><searchLink fieldCode="DE" term="%22Two-Dimensional%2Fmethods%22">Two-Dimensional/methods</searchLink><br /><searchLink fieldCode="DE" term="%22Humans%22">Humans</searchLink><br /><searchLink fieldCode="DE" term="%22Isoelectric+Focusing%22">Isoelectric Focusing</searchLink><br /><searchLink fieldCode="DE" term="%22Liver+Neoplasms%2Fblood%22">Liver Neoplasms/blood</searchLink><br /><searchLink fieldCode="DE" term="%22Peptide+Mapping%22">Peptide Mapping</searchLink><br /><searchLink fieldCode="DE" term="%22Protein+Array+Analysis%2Fmethods%22">Protein Array Analysis/methods</searchLink><br /><searchLink fieldCode="DE" term="%22Proteome%2Fisolation+%26+purification%22">Proteome/isolation & purification</searchLink><br /><searchLink fieldCode="DE" term="%22Proteomics%2Fmethods%22">Proteomics/methods</searchLink><br /><searchLink fieldCode="DE" term="%22Spectrometry%22">Spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Mass%22">Mass</searchLink><br /><searchLink fieldCode="DE" term="%22Matrix-Assisted+Laser+Desorption-Ionization%22">Matrix-Assisted Laser Desorption-Ionization</searchLink><br /><searchLink fieldCode="DE" term="%22Staining+and+Labeling%22">Staining and Labeling</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Human plasma is regarded the most complex and well-known clinical specimen that can be easily obtained; alterations in the levels of plasma proteins or their corresponding enzyme activities may reflect either a healthy or a diseased state. Given that there is no defined genomic information as to the intact protein components in plasma, protein profiling could be the first step toward its molecular characterization. Several problems exist in the analysis of plasma proteins, however. For example, the widest dynamic range of protein concentrations, the presence of high-abundance proteins, and post-translational modifications need to be considered before proteomic studies are undertaken. In particular, efficient depletion or pre-fractionation of high-abundance proteins is crucial for the identification of low-abundance proteins that may contain potential biomarkers. After the removal of high-abundance proteins, protein profiling can be initiated using two-dimensional electrophoresis (2DE), which has been widely used for displaying the differential proteome under specific physiological conditions. Here, we describe a typical 2DE procedure for plasma proteome under either a healthy or a diseased state (e.g., liver cancer) in which pre-fractionation and depletion are integral steps in the search for disease biomarkers. ; open
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  Data: 57~75
– Name: Language
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  Data: unknown
– Name: NoteTitleSource
  Label: Relation
  Group: SrcInfo
  Data: Methods in Molecular Biology (Clifton, N.J.); J02226; https://ir.ymlib.yonsei.ac.kr/handle/22282913/107578; T200801286
– Name: URL
  Label: Availability
  Group: URL
  Data: https://ir.ymlib.yonsei.ac.kr/handle/22282913/107578<br />http://link.springer.com/protocol/10.1007%2F978-1-59745-117-8_4
– Name: Copyright
  Label: Rights
  Group: Cpyrght
  Data: CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ ; not free
– Name: AN
  Label: Accession Number
  Group: ID
  Data: edsbas.2213C98F
PLink https://erproxy.cvtisr.sk/sfx/access?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsbas&AN=edsbas.2213C98F
RecordInfo BibRecord:
  BibEntity:
    Languages:
      – Text: unknown
    Subjects:
      – SubjectFull: Biomarkers
        Type: general
      – SubjectFull: Tumor/blood
        Type: general
      – SubjectFull: Blood Protein Electrophoresis/methods
        Type: general
      – SubjectFull: Blood Proteins/isolation & purification
        Type: general
      – SubjectFull: Carcinoma
        Type: general
      – SubjectFull: Hepatocellular/blood
        Type: general
      – SubjectFull: Chromatography
        Type: general
      – SubjectFull: Affinity
        Type: general
      – SubjectFull: High Pressure Liquid
        Type: general
      – SubjectFull: Electrophoresis
        Type: general
      – SubjectFull: Gel
        Type: general
      – SubjectFull: Two-Dimensional/methods
        Type: general
      – SubjectFull: Humans
        Type: general
      – SubjectFull: Isoelectric Focusing
        Type: general
      – SubjectFull: Liver Neoplasms/blood
        Type: general
      – SubjectFull: Peptide Mapping
        Type: general
      – SubjectFull: Protein Array Analysis/methods
        Type: general
      – SubjectFull: Proteome/isolation & purification
        Type: general
      – SubjectFull: Proteomics/methods
        Type: general
      – SubjectFull: Spectrometry
        Type: general
      – SubjectFull: Mass
        Type: general
      – SubjectFull: Matrix-Assisted Laser Desorption-Ionization
        Type: general
      – SubjectFull: Staining and Labeling
        Type: general
    Titles:
      – TitleFull: Protein profiling of human plasma samples by two-dimensional electrophoresis.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Sang Yun Cho
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            NameFull: Eun-Young Lee
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            NameFull: Hye-Young Kim
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            NameFull: Min-Jung Kang
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            NameFull: Hyoung-Joo Lee
      – PersonEntity:
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            NameFull: Hoguen Kim
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            NameFull: Young-Ki Paik
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            NameFull: Kim, Ho Keun
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          Dates:
            – D: 01
              M: 01
              Type: published
              Y: 2008
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