Comparison of different culture media and storage temperatures for the long-term preservation of Streptococcus pneumoniae in the tropics
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| Title: | Comparison of different culture media and storage temperatures for the long-term preservation of Streptococcus pneumoniae in the tropics |
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| Authors: | George K. Siberry, T. Jacob John, Rajeswar Pandian, Brahmadathan Kn, M. K. Lalitha, Mark C. Steinhoff |
| Source: | Bulletin of the World Health Organization, Vol 79, Iss 1, Pp 43-47 (2001) |
| Publisher Information: | The World Health Organization, 2001. |
| Publication Year: | 2001 |
| Subject Terms: | culture media, 0301 basic medicine, Cryopreservation, 0303 health sciences, Tropical Climate, desiccation/methods, tropical climate, Cost-Benefit Analysis, Preservation, Biological, Cell Culture Techniques, India, cryopreservation/methods, 3. Good health, Culture Media, Specimen Handling, 03 medical and health sciences, Streptococcus pneumoniae, Streptococcus pneumoniae/preservation, Humans, lyophilization/methods, Public aspects of medicine, RA1-1270 |
| Description: | The preservation of Streptococcus pneumoniae by standard freezing methods for subsequent tests--such as serotyping and antibiotic susceptibility--is not possible or is difficult in many developing countries because of the high cost of equipment, inadequate equipment maintenance, and irregular power supply. We evaluated alternative low-cost methods, by comparing different culture media and storage temperatures.Clinical isolates of five capsular types (1, 5, 7, 19, and 23) of S. pneumoniae were preserved in rabbit blood, sheep blood, skimmed milk, or glycerol-chocolate broth, and stored at -20 degrees C or -70 degrees C. The cultures were also preserved by lyophilization or sand desiccation, followed by storage at room temperature and 4 degrees C. The viability of the preserved cultures was determined by making serial colony counts on day 0 and after 1 week, 4 weeks, 4 months and 16 months. The viability of cultures preserved by sand desiccation and storage at 4 degrees C was also determined every 6 months for up to 68 months.Irrespective of the media used, cultures maintained at -20 degrees C became nonviable by the fourth month, while those maintained at -70 degrees C were still viable at 16 months. Cultures preserved by lyophilization or sand desiccation lost their viability by the fourth month when maintained at local room temperature (30-42 degrees C), but remained viable when stored at 4 degrees C for up to 68 months.Our results confirm that freezing at -70 degrees C, or lyophilization and storage at 4 degrees C are the ideal methods for the preservation of S. pneumoniae. In laboratories where lyophilization is not feasible, sand desiccation and storage at 4 degrees C offers an alternative low-cost method for the long-term preservation of S. pneumoniae. |
| Document Type: | Article |
| Language: | English |
| ISSN: | 0042-9686 |
| DOI: | 10.1590/s0042-96862001000100009 |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/11217666 https://doaj.org/article/b5befd9ddcc44bd3a32c38755209a819 https://scielosp.org/pdf/bwho/2001.v79n1/43-47 http://europepmc.org/articles/PMC2566344 http://repository.ias.ac.in/29580/1/408.pdf https://www.who.int/entity/bulletin/archives/79(1)43.pdf https://jhu.pure.elsevier.com/en/publications/comparison-of-different-culture-media-and-storage-temperatures-fo-5 https://www.who.int/bulletin/archives/79(1)43.pdf |
| Accession Number: | edsair.pmid.dedup....afb3d5d772e8bd016190de8de7d56a7c |
| Database: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | The preservation of Streptococcus pneumoniae by standard freezing methods for subsequent tests--such as serotyping and antibiotic susceptibility--is not possible or is difficult in many developing countries because of the high cost of equipment, inadequate equipment maintenance, and irregular power supply. We evaluated alternative low-cost methods, by comparing different culture media and storage temperatures.Clinical isolates of five capsular types (1, 5, 7, 19, and 23) of S. pneumoniae were preserved in rabbit blood, sheep blood, skimmed milk, or glycerol-chocolate broth, and stored at -20 degrees C or -70 degrees C. The cultures were also preserved by lyophilization or sand desiccation, followed by storage at room temperature and 4 degrees C. The viability of the preserved cultures was determined by making serial colony counts on day 0 and after 1 week, 4 weeks, 4 months and 16 months. The viability of cultures preserved by sand desiccation and storage at 4 degrees C was also determined every 6 months for up to 68 months.Irrespective of the media used, cultures maintained at -20 degrees C became nonviable by the fourth month, while those maintained at -70 degrees C were still viable at 16 months. Cultures preserved by lyophilization or sand desiccation lost their viability by the fourth month when maintained at local room temperature (30-42 degrees C), but remained viable when stored at 4 degrees C for up to 68 months.Our results confirm that freezing at -70 degrees C, or lyophilization and storage at 4 degrees C are the ideal methods for the preservation of S. pneumoniae. In laboratories where lyophilization is not feasible, sand desiccation and storage at 4 degrees C offers an alternative low-cost method for the long-term preservation of S. pneumoniae. |
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| ISSN: | 00429686 |
| DOI: | 10.1590/s0042-96862001000100009 |