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Quantification of noradrenergic‐, dopaminergic‐, and tectal‐neurons during aging in the short‐lived killifish Nothobranchius furzeri

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Názov: Quantification of noradrenergic‐, dopaminergic‐, and tectal‐neurons during aging in the short‐lived killifish Nothobranchius furzeri
Autori: Bagnoli, Sara, Fronte, Baldassare, Bibbiani, Carlo, Terzibasi Tozzini, Eva, Cellerino, Alessandro
Informácie o vydavateľovi: 2022.
Rok vydania: 2022
Predmety: Parkinson Disease/metabolism [MeSH], Parkinson Disease/metabolism, Dopaminergic Neurons/metabolism [MeSH], Aging/genetics [MeSH], Dopamine/metabolism, Animals [MeSH], Parkinson Disease/genetics [MeSH], Fundulidae/metabolism [MeSH], Dopamine/metabolism [MeSH], Aging/genetics, Parkinson Disease/genetics, Dopaminergic Neurons/metabolism, Norepinephrine/metabolism, Animals, Norepinephrine/metabolism [MeSH], Fundulidae/metabolism
Popis: Parkinson's disease (PD) is characterized by phosphorylation and aggregation of the protein α-Synuclein and ensuing neuronal death progressing from the noradrenergic locus coeruleus to midbrain dopaminergic neurons. In 2019, Matsui and colleagues reported a spontaneous age-dependent degeneration of dopaminergic neurons and an even greater neurodegeneration of the noradrenergic neurons in the short-lived killifish Nothobranchius furzeri. Given the great possible relevance of a spontaneous model for PD, we assessed neurodegeneration of noradrenergic and dopaminergic neurons in two further laboratory strains of N. furzeri. We implemented, for the first time in N. furzeri, a whole-brain clarification technique and proceeded to entire 3D nuclei reconstruction to quantify total cell numbers in two different stains of N. furzeri. In both strains, we observed that age-dependent neurodegeneration is limited to the locus coeruleus and does not involve the posterior tuberculum. We also applied 3D counting to the optic tectum, an area of active adult neurogenesis, and detected an increase of neurons with age. Our results confirm age-dependent neurodegeneration of noradrenergic neurons, a condition reminiscent of the presymptomatic stage of PD indicating that N. furzeri could be used in the future to identify modifying factors for age-dependent neurodegeneration and open the intriguing possibility that natural genetic variation may influence the susceptibility of dopaminergic neurons.
Druh dokumentu: Article
Jazyk: English
DOI: 10.1111/acel.13689
Prístupová URL adresa: https://onlinelibrary.wiley.com/doi/10.1111/acel.13689#support-information-section
Prístupové číslo: edsair.od......3694..8f93c1f117afad1f11b3e0eaef940ebe
Databáza: OpenAIRE
Popis
Abstrakt:Parkinson's disease (PD) is characterized by phosphorylation and aggregation of the protein α-Synuclein and ensuing neuronal death progressing from the noradrenergic locus coeruleus to midbrain dopaminergic neurons. In 2019, Matsui and colleagues reported a spontaneous age-dependent degeneration of dopaminergic neurons and an even greater neurodegeneration of the noradrenergic neurons in the short-lived killifish Nothobranchius furzeri. Given the great possible relevance of a spontaneous model for PD, we assessed neurodegeneration of noradrenergic and dopaminergic neurons in two further laboratory strains of N. furzeri. We implemented, for the first time in N. furzeri, a whole-brain clarification technique and proceeded to entire 3D nuclei reconstruction to quantify total cell numbers in two different stains of N. furzeri. In both strains, we observed that age-dependent neurodegeneration is limited to the locus coeruleus and does not involve the posterior tuberculum. We also applied 3D counting to the optic tectum, an area of active adult neurogenesis, and detected an increase of neurons with age. Our results confirm age-dependent neurodegeneration of noradrenergic neurons, a condition reminiscent of the presymptomatic stage of PD indicating that N. furzeri could be used in the future to identify modifying factors for age-dependent neurodegeneration and open the intriguing possibility that natural genetic variation may influence the susceptibility of dopaminergic neurons.
DOI:10.1111/acel.13689