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Studium cytostatických účinků sirného yperitu

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Title: Studium cytostatických účinků sirného yperitu
Authors: Minaříková, Martina
Contributors: Pourová, Jana, Vokřál, Ivan
Publisher Information: 2025.
Publication Year: 2025
Subject Terms: buněčná linie HaCaT, cell cycle synchronization, sulfur mustard, cell cycle, buněčná viabilita, HaCaT cell lines, buněčný cyklus, sirný yperit, cell viability, synchronizace buněčného cyklu
Description: Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Martina Krejčová Supervisor: doc. PharmDr. Jana Pourová, Ph.D. Consultant: Mgr. Petr Jošt. Ph.D. Title of diploma thesis: Study of the cytostatic effects of sulfur mustard Sulfur mustard (HD) belongs to blistering agents used in chemical warfare. It is a bifunctional alkylating agent that covalently modifies DNA. The cytostatic effect of HD is characterized by halting cell division without cell death. The duration for which a cell can remain in this state depends on the extent of DNA damage, which can subsequently be converted into a cytotoxic effect leading to cell death. The aim of the study was to determine the concentration range of HD gas that exhibits cytostatic effects and to investigate the sensitivity of HaCaT skin keratinocyte cells synchronized in the G1, S, and G2/M phases of the cell cycle to alkylating damage induction following release from cell cycle blockage. The cytostatic effect was observed only at very low concentrations, in the range of units of µmol.l-1 . In our experiments, a concentration of 2 µmol.l-1 of HD prevented cell proliferation for 2 days. Lower, submicromolar concentrations, had a stimulatory effect on cell proliferation, while higher concentrations of HD...
Document Type: Doctoral thesis
Language: Czech
Access URL: http://www.nusl.cz/ntk/nusl-669676
Accession Number: edsair.od......2186..7c00d4f6dbb7d814fc98b0a63b6c0e87
Database: OpenAIRE
Description
Abstract:Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Martina Krejčová Supervisor: doc. PharmDr. Jana Pourová, Ph.D. Consultant: Mgr. Petr Jošt. Ph.D. Title of diploma thesis: Study of the cytostatic effects of sulfur mustard Sulfur mustard (HD) belongs to blistering agents used in chemical warfare. It is a bifunctional alkylating agent that covalently modifies DNA. The cytostatic effect of HD is characterized by halting cell division without cell death. The duration for which a cell can remain in this state depends on the extent of DNA damage, which can subsequently be converted into a cytotoxic effect leading to cell death. The aim of the study was to determine the concentration range of HD gas that exhibits cytostatic effects and to investigate the sensitivity of HaCaT skin keratinocyte cells synchronized in the G1, S, and G2/M phases of the cell cycle to alkylating damage induction following release from cell cycle blockage. The cytostatic effect was observed only at very low concentrations, in the range of units of µmol.l-1 . In our experiments, a concentration of 2 µmol.l-1 of HD prevented cell proliferation for 2 days. Lower, submicromolar concentrations, had a stimulatory effect on cell proliferation, while higher concentrations of HD...