View in EDS

Bacterial Vaginosis-Associated Prevotella timonensis Enhances Dendritic Cell-T Cell Clustering and Subsequent T Cell Proliferation

Saved in:
Bibliographic Details
Title: Bacterial Vaginosis-Associated Prevotella timonensis Enhances Dendritic Cell-T Cell Clustering and Subsequent T Cell Proliferation
Authors: van Smoorenburg, Marleen Y, Nerwinska, Julia L, van Hamme, John L, Remmerswaal, Ester B M, Segui-Perez, Celia, Strijbis, Karin, Geijtenbeek, Teunis B H
Source: European Journal of Immunology. 55(9):1-8
Publisher Information: Wiley, 2025.
Publication Year: 2025
Subject Terms: Dendritic Cells/immunology, Vaginosis, Bacterial/immunology, CD4-Positive T-Lymphocytes/immunology, Humans, Monocytes/immunology, Female, Prevotella/immunology, Lymphocyte Activation/immunology, Cell Proliferation, Vagina/microbiology
Description: Dysbiosis of the vaginal microbiome is associated with increased inflammation in the female genital tract. Microbiota associated with bacterial vaginosis (BV), such as Gardnerella vaginalis, Megasphaera elsdenii, and Prevotella timonensis, replace the health-associated bacterium Lactobacillus crispatus and cause inflammation affecting mucosal integrity and immunity. However, it remains unclear how these BV-associated bacteria modulate immune cells and enhance inflammation. Here, we investigated whether BV-associated bacteria directly affected dendritic cell (DC) function. Notably, P. timonensis but not M. elsdenii induced cell-cell clustering between monocytic cell lines and, importantly, between primary DCs and primary CD4 T cells. Our data indicate that this increased clustering is independent of LFA-1. Moreover, P. timonensis enhanced DC-mediated CD4 T cell proliferation. Altogether, these results suggest that P. timonensis-induced cell-cell clustering contributes to the elevated mucosal inflammation observed during bacterial vaginosis.
Document Type: Article
Language: English
ISSN: 1521-4141
0014-2980
DOI: 10.1002/eji.70051
Access URL: https://research-portal.uu.nl/en/publications/3d4fa1a2-175c-44fe-8503-7c14d50fe12d
Rights: CC BY
Accession Number: edsair.dris...02462..3ab0d53d4e109eb94449b078c9c8ad21
Database: OpenAIRE
Description
Abstract:Dysbiosis of the vaginal microbiome is associated with increased inflammation in the female genital tract. Microbiota associated with bacterial vaginosis (BV), such as Gardnerella vaginalis, Megasphaera elsdenii, and Prevotella timonensis, replace the health-associated bacterium Lactobacillus crispatus and cause inflammation affecting mucosal integrity and immunity. However, it remains unclear how these BV-associated bacteria modulate immune cells and enhance inflammation. Here, we investigated whether BV-associated bacteria directly affected dendritic cell (DC) function. Notably, P. timonensis but not M. elsdenii induced cell-cell clustering between monocytic cell lines and, importantly, between primary DCs and primary CD4 T cells. Our data indicate that this increased clustering is independent of LFA-1. Moreover, P. timonensis enhanced DC-mediated CD4 T cell proliferation. Altogether, these results suggest that P. timonensis-induced cell-cell clustering contributes to the elevated mucosal inflammation observed during bacterial vaginosis.
ISSN:15214141
00142980
DOI:10.1002/eji.70051