Removal of PSA from NCAM affects the survival of magnocellular vasopressin‐ and oxytocin‐producing neurons in organotypic cultures of the paraventricular nucleus
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| Názov: | Removal of PSA from NCAM affects the survival of magnocellular vasopressin‐ and oxytocin‐producing neurons in organotypic cultures of the paraventricular nucleus |
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| Autori: | Vutskits, Laszlo, Gascon, Eduardo, Kiss, Jozsef Zoltan |
| Zdroj: | European Journal of Neuroscience, Vol. 17, No 10 (2003) pp. 2119-2126 |
| Informácie o vydavateľovi: | Wiley, 2003. |
| Rok vydania: | 2003 |
| Predmety: | Male, 0301 basic medicine, 616.8, Glycoside Hydrolases, Paraventricular Hypothalamic Nucleus/ cytology, Cell Survival, Oxytocin/biosynthesis, Neural Cell Adhesion Molecule L1, Oxytocin, Leukemia Inhibitory Factor, Rats, Sprague-Dawley, Neural Cell Adhesion Molecules/ metabolism, 03 medical and health sciences, Organ Culture Techniques, Animals, Ciliary Neurotrophic Factor, Neural Cell Adhesion Molecules, Cell Survival/physiology, Neurons, Lymphokines, 0303 health sciences, Neuronal Plasticity, Interleukin-6, Ciliary Neurotrophic Factor/pharmacology, Membrane Proteins, Vasopressins/biosynthesis, Lymphokines/pharmacology, Neuronal Plasticity/physiology, Neurons/ cytology/drug effects/ metabolism, Neural Cell Adhesion Molecule L1/ metabolism, Growth Inhibitors, ddc:616.8, Rats, Glycoside Hydrolases/pharmacology, Sialic Acids/ metabolism, Sialic Acids, Membrane Proteins/metabolism, Growth Inhibitors/pharmacology, Paraventricular Hypothalamic Nucleus |
| Popis: | The expression of the polysialic acid neural cell adhesion molecule (PSA‐NCAM) in the hypothalamo‐neurohypophyseal system has been correlated with morphofunctional plasticity. In this study, we investigated the role of PSA‐NCAM in the survival of oxytocin (OT)‐ and vasopressin (VP)‐producing magnocellular cells of this system. We used a recently developed organotypic slice culture model of the rat hypothalamic paraventricular nucleus (PVN) in which ciliary neurotrophic factor (CNTF) and leukemia inhibitory factor (LIF) are potent survival factors for magnocellular neurons. We demonstrate by means of confocal microscopy that cultured magnocellular VP and OT neurons express strong immunoreactivity for PSA‐NCAM. Removal of PSA from NCAM by the enzyme Endo N leads to a significant loss of both VP and OT neurons in the presence of low concentrations of CNTF. Endo N treatment did not change cell survival in the presence of LIF. These results suggest that, in addition to its role in neuro‐glial plasticity, PSA‐NCAM might also influence the trophic factor responsiveness of hypothalamic VP and OT neurosecretory cells. |
| Druh dokumentu: | Article |
| Popis súboru: | application/pdf |
| Jazyk: | English |
| ISSN: | 1460-9568 0953-816X |
| DOI: | 10.1046/j.1460-9568.2003.02660.x |
| Prístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/12786978 https://core.ac.uk/display/60786724 https://archive-ouverte.unige.ch/unige:10506 https://www.ncbi.nlm.nih.gov/pubmed/12786978 https://europepmc.org/article/MED/12786978 https://archive-ouverte.unige.ch/unige:10506 |
| Rights: | Wiley Online Library User Agreement |
| Prístupové číslo: | edsair.doi.dedup.....fec7d9ec69d22f54eeb0be5ce9f4fb3b |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | The expression of the polysialic acid neural cell adhesion molecule (PSA‐NCAM) in the hypothalamo‐neurohypophyseal system has been correlated with morphofunctional plasticity. In this study, we investigated the role of PSA‐NCAM in the survival of oxytocin (OT)‐ and vasopressin (VP)‐producing magnocellular cells of this system. We used a recently developed organotypic slice culture model of the rat hypothalamic paraventricular nucleus (PVN) in which ciliary neurotrophic factor (CNTF) and leukemia inhibitory factor (LIF) are potent survival factors for magnocellular neurons. We demonstrate by means of confocal microscopy that cultured magnocellular VP and OT neurons express strong immunoreactivity for PSA‐NCAM. Removal of PSA from NCAM by the enzyme Endo N leads to a significant loss of both VP and OT neurons in the presence of low concentrations of CNTF. Endo N treatment did not change cell survival in the presence of LIF. These results suggest that, in addition to its role in neuro‐glial plasticity, PSA‐NCAM might also influence the trophic factor responsiveness of hypothalamic VP and OT neurosecretory cells. |
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| ISSN: | 14609568 0953816X |
| DOI: | 10.1046/j.1460-9568.2003.02660.x |