Connexin 43 Hemichannels Contribute to Cytoplasmic Ca2+ Oscillations by Providing a Bimodal Ca2+-dependent Ca2+ Entry Pathway
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| Názov: | Connexin 43 Hemichannels Contribute to Cytoplasmic Ca2+ Oscillations by Providing a Bimodal Ca2+-dependent Ca2+ Entry Pathway |
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| Autori: | De Bock, Marijke, Wang, Nan, Bol, Mélissa, Decrock, Elke, Ponsaerts, Raf, Bultynck, Geert, Dupont, Geneviève, Leybaert, Luc |
| Zdroj: | The Journal of biological chemistry, 287 (15 |
| Informácie o vydavateľovi: | Elsevier BV, 2012. |
| Rok vydania: | 2012 |
| Predmety: | Calcium Channel Blockers -- pharmacology, 0301 basic medicine, Cytoplasm, Inositol 1,4,5-Trisphosphate -- metabolism, Peptides -- pharmacology, Inositol 1,4,5-Trisphosphate, Bradykinin, Connexins, Cell Line, 03 medical and health sciences, Adenosine Triphosphate, Dogs, Chimie, Animals, Humans, Calcium Signaling, Fluoresceins -- metabolism, Recombinant Proteins -- metabolism, 0303 health sciences, Calcium Channels -- metabolism, Carbenoxolone -- pharmacology, Connexins -- metabolism, Oligopeptides -- pharmacology, Cytochromes c, Calcium Channel Blockers, Fluoresceins, Rats, Connexin 43 -- genetics -- metabolism, Cytoplasm -- metabolism, Bradykinin -- pharmacology, Adenosine Triphosphate -- metabolism -- pharmacology, Connexin 43, Gene Knockdown Techniques, Cytochromes c -- metabolism -- physiology, Carbenoxolone, Calcium -- metabolism, RNA Interference, Calcium, Calcium Channels, Peptides, Oligopeptides |
| Popis: | Many cellular functions are driven by changes in the intracellular Ca(2+) concentration ([Ca(2+)](i)) that are highly organized in time and space. Ca(2+) oscillations are particularly important in this respect and are based on positive and negative [Ca(2+)](i) feedback on inositol 1,4,5-trisphosphate receptors (InsP(3)Rs). Connexin hemichannels are Ca(2+)-permeable plasma membrane channels that are also controlled by [Ca(2+)](i). We aimed to investigate how hemichannels may contribute to Ca(2+) oscillations. Madin-Darby canine kidney cells expressing connexin-32 (Cx32) and Cx43 were exposed to bradykinin (BK) or ATP to induce Ca(2+) oscillations. BK-induced oscillations were rapidly (minutes) and reversibly inhibited by the connexin-mimetic peptides (32)Gap27/(43)Gap26, whereas ATP-induced oscillations were unaffected. Furthermore, these peptides inhibited the BK-triggered release of calcein, a hemichannel-permeable dye. BK-induced oscillations, but not those induced by ATP, were dependent on extracellular Ca(2+). Alleviating the negative feedback of [Ca(2+)](i) on InsP(3)Rs using cytochrome c inhibited BK- and ATP-induced oscillations. Cx32 and Cx43 hemichannels are activated by |
| Druh dokumentu: | Article |
| Popis súboru: | 2 full-text file(s): application/pdf; application/pdf |
| Jazyk: | English |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m111.299610 |
| Prístupová URL adresa: | http://www.jbc.org/content/287/15/12250.full.pdf https://pubmed.ncbi.nlm.nih.gov/22351781 https://pubmed.ncbi.nlm.nih.gov/22351781/ https://europepmc.org/articles/PMC3320976 https://difusion.ulb.ac.be/vufind/Record/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/146309/Details https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3320976/ https://www.sciencedirect.com/science/article/pii/S0021925820531542 https://www.jbc.org/article/S0021-9258(20)53154-2/fulltext http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/146309 |
| Rights: | CC BY |
| Prístupové číslo: | edsair.doi.dedup.....e7f1910a94e056b0e3a572bdcc2f5030 |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Many cellular functions are driven by changes in the intracellular Ca(2+) concentration ([Ca(2+)](i)) that are highly organized in time and space. Ca(2+) oscillations are particularly important in this respect and are based on positive and negative [Ca(2+)](i) feedback on inositol 1,4,5-trisphosphate receptors (InsP(3)Rs). Connexin hemichannels are Ca(2+)-permeable plasma membrane channels that are also controlled by [Ca(2+)](i). We aimed to investigate how hemichannels may contribute to Ca(2+) oscillations. Madin-Darby canine kidney cells expressing connexin-32 (Cx32) and Cx43 were exposed to bradykinin (BK) or ATP to induce Ca(2+) oscillations. BK-induced oscillations were rapidly (minutes) and reversibly inhibited by the connexin-mimetic peptides (32)Gap27/(43)Gap26, whereas ATP-induced oscillations were unaffected. Furthermore, these peptides inhibited the BK-triggered release of calcein, a hemichannel-permeable dye. BK-induced oscillations, but not those induced by ATP, were dependent on extracellular Ca(2+). Alleviating the negative feedback of [Ca(2+)](i) on InsP(3)Rs using cytochrome c inhibited BK- and ATP-induced oscillations. Cx32 and Cx43 hemichannels are activated by |
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| ISSN: | 00219258 |
| DOI: | 10.1074/jbc.m111.299610 |