Complete chloroplast genome sequence and characteristics analysis of Qingda no.1 alfalfa (Medicago sativa L. cv. Qingda no.1)

Saved in:
Bibliographic Details
Title: Complete chloroplast genome sequence and characteristics analysis of Qingda no.1 alfalfa (Medicago sativa L. cv. Qingda no.1)
Authors: Yuling Ren, Yajun Ma, Xue Li, Xiaoan Li, Guozhu Yang, Ping Li
Source: Czech Journal of Genetics and Plant Breeding, Vol 59, Iss 3, Pp 160-168 (2023)
Publisher Information: Czech Academy of Agricultural Sciences, 2023.
Publication Year: 2023
Subject Terms: 0301 basic medicine, 0303 health sciences, 03 medical and health sciences, phylogenetic analysis, chloroplast genome sequencing, Plant culture, 15. Life on land, qinghai-tibet plateau, medicago sativa l. cv. qingda no.1, SB1-1110
Description: Medicago sativa is the most widely cultivated forage legume and one of the most economically valuable crops throughout the world. Qingda no.1 (Medicago sativa L. cv. Qingda no.1) is an excellent alfalfa local variety with strong cold, drought and salt resistance in the three rivers source area of Qinghai. In this study, the whole chloroplast (cp) genome of Qingda no.1 was sequenced, assembled and its structure was analysed by the Illumina high-throughput sequencing technology. The results showed that the chloroplast genome of Qingda no.1 exhibits no obvious typical quadripartite structure; the total length of the chloroplast genome is 125 637 bp; the chloroplast genome contained 111 genes, including 77 protein-coding genes, 30 tRNA genes, and 4 rRNA genes, with an overall GC content of 38.33%. The relative synonymous codon usage showed that 68.67% of the codons RSCU > 1 in Qingda no.1, with the preference ending with A and T. The simple sequence repeat (SSR) analysis identified 62 SSR loci. The phylogenetic analysis of the cp genome, Qingda no.1 clustered closely with Medicago sativa KU321683 (Medicago sativa L. cv. KU321683). These results are helpful for the further study of the Qingda no.1 adaptation mechanism to high altitude stress environments.
Document Type: Article
ISSN: 1805-9325
1212-1975
DOI: 10.17221/71/2022-cjgpb
Access URL: https://doaj.org/article/95eb6004d1234ced82b2236c555b0587
Rights: CC BY NC
Accession Number: edsair.doi.dedup.....d105d9fcd3d1d82c7ba7548117b03f7a
Database: OpenAIRE
Be the first to leave a comment!
You must be logged in first