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Purification and Characterization of Two Voltage-Dependent Anion Channel Isoforms from Plant Seeds

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Názov: Purification and Characterization of Two Voltage-Dependent Anion Channel Isoforms from Plant Seeds
Autori: Abrecht, H, Wattiez, R, Ruysschaert, Jean Marie, Homblé, Fabrice
Zdroj: Plant Physiology. 124:1181-1190
Informácie o vydavateľovi: Oxford University Press (OUP), 2000.
Rok vydania: 2000
Predmety: Fabaceae -- ultrastructure, 0301 basic medicine, Seeds -- metabolism, Lipid Bilayers, Molecular Sequence Data, Porins, Ion Channels -- chemistry, Medicinal, Peptide Mapping, Ion Channels, 03 medical and health sciences, Mitochondria -- metabolism, Sequence Analysis, Protein, Fabaceae -- chemistry, Lipid Bilayers -- chemistry, Phosphatidylcholines -- chemistry, Plant Proteins -- isolation & purification, Protein Isoforms, Voltage-Dependent Anion Channels, Amino Acid Sequence, Plant Proteins, 0303 health sciences, Porins -- chemistry, Plants, Medicinal, Ion Channels -- isolation & purification, Protein, Fabaceae, Mitochondria -- chemistry, Sciences bio-médicales et agricoles, Plants, Seeds -- ultrastructure, Mitochondria, Protein Isoforms -- chemistry, Porins -- isolation & purification, Seeds, Phosphatidylcholines, Protein Isoforms -- isolation & purification, Fabaceae -- metabolism, Seeds -- chemistry, Sequence Analysis, Sequence Alignment, Plant Proteins -- chemistry
Popis: Mitochondria were isolated from imbibed seeds of lentil (Lens culinaris) and Phaseolus vulgaris. We copurified two voltage-dependent anion channel from detergent solubilized mitochondria in a single purification step using hydroxyapatite. The two isoforms from P. vulgaris were separated by chromatofocusing chromatography in 4m urea without any loss of channel activity. Channel activity of each isoform was characterized upon reconstitution into diphytanoyl phosphatidylcholine planar lipid bilayers. Both isoforms form large conductance channels that are slightly anion selective and display cation selective substates.
Druh dokumentu: Article
Popis súboru: 1 full-text file(s): application/pdf
Jazyk: English
ISSN: 1532-2548
0032-0889
DOI: 10.1104/pp.124.3.1181
Prístupová URL adresa: http://www.plantphysiol.org/content/124/3/1181.full.pdf
https://pubmed.ncbi.nlm.nih.gov/11080295
https://difusion.ulb.ac.be/vufind/Record/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/58318/Details
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC59217/
https://core.ac.uk/display/8857882
https://academic.oup.com/plphys/article/124/3/1181/6097514
https://www.jstor.org/stable/4279521
http://www.plantphysiol.org/content/124/3/1181
Rights: OUP Standard Publication Reuse
Prístupové číslo: edsair.doi.dedup.....bb6b72e0ac1e83423b74f0e52f5d209c
Databáza: OpenAIRE
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  Data: Purification and Characterization of Two Voltage-Dependent Anion Channel Isoforms from Plant Seeds
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  Data: <searchLink fieldCode="AR" term="%22Abrecht%2C+H%22">Abrecht, H</searchLink><br /><searchLink fieldCode="AR" term="%22Wattiez%2C+R%22">Wattiez, R</searchLink><br /><searchLink fieldCode="AR" term="%22Ruysschaert%2C+Jean+Marie%22">Ruysschaert, Jean Marie</searchLink><br /><searchLink fieldCode="AR" term="%22Homblé%2C+Fabrice%22">Homblé, Fabrice</searchLink>
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  Data: <i>Plant Physiology</i>. 124:1181-1190
– Name: Publisher
  Label: Publisher Information
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  Data: Oxford University Press (OUP), 2000.
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  Data: 2000
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  Data: <searchLink fieldCode="DE" term="%22Fabaceae+--+ultrastructure%22">Fabaceae -- ultrastructure</searchLink><br /><searchLink fieldCode="DE" term="%220301+basic+medicine%22">0301 basic medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Seeds+--+metabolism%22">Seeds -- metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22Lipid+Bilayers%22">Lipid Bilayers</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+Sequence+Data%22">Molecular Sequence Data</searchLink><br /><searchLink fieldCode="DE" term="%22Porins%22">Porins</searchLink><br /><searchLink fieldCode="DE" term="%22Ion+Channels+--+chemistry%22">Ion Channels -- chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Medicinal%22">Medicinal</searchLink><br /><searchLink fieldCode="DE" term="%22Peptide+Mapping%22">Peptide Mapping</searchLink><br /><searchLink fieldCode="DE" term="%22Ion+Channels%22">Ion Channels</searchLink><br /><searchLink fieldCode="DE" term="%2203+medical+and+health+sciences%22">03 medical and health sciences</searchLink><br /><searchLink fieldCode="DE" term="%22Mitochondria+--+metabolism%22">Mitochondria -- metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22Sequence+Analysis%2C+Protein%22">Sequence Analysis, Protein</searchLink><br /><searchLink fieldCode="DE" term="%22Fabaceae+--+chemistry%22">Fabaceae -- chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Lipid+Bilayers+--+chemistry%22">Lipid Bilayers -- chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Phosphatidylcholines+--+chemistry%22">Phosphatidylcholines -- chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+Proteins+--+isolation+%26+purification%22">Plant Proteins -- isolation & purification</searchLink><br /><searchLink fieldCode="DE" term="%22Protein+Isoforms%22">Protein Isoforms</searchLink><br /><searchLink fieldCode="DE" term="%22Voltage-Dependent+Anion+Channels%22">Voltage-Dependent Anion Channels</searchLink><br /><searchLink fieldCode="DE" term="%22Amino+Acid+Sequence%22">Amino Acid Sequence</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+Proteins%22">Plant Proteins</searchLink><br /><searchLink fieldCode="DE" term="%220303+health+sciences%22">0303 health sciences</searchLink><br /><searchLink fieldCode="DE" term="%22Porins+--+chemistry%22">Porins -- chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Plants%2C+Medicinal%22">Plants, Medicinal</searchLink><br /><searchLink fieldCode="DE" term="%22Ion+Channels+--+isolation+%26+purification%22">Ion Channels -- isolation & purification</searchLink><br /><searchLink fieldCode="DE" term="%22Protein%22">Protein</searchLink><br /><searchLink fieldCode="DE" term="%22Fabaceae%22">Fabaceae</searchLink><br /><searchLink fieldCode="DE" term="%22Mitochondria+--+chemistry%22">Mitochondria -- chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Sciences+bio-médicales+et+agricoles%22">Sciences bio-médicales et agricoles</searchLink><br /><searchLink fieldCode="DE" term="%22Plants%22">Plants</searchLink><br /><searchLink fieldCode="DE" term="%22Seeds+--+ultrastructure%22">Seeds -- ultrastructure</searchLink><br /><searchLink fieldCode="DE" term="%22Mitochondria%22">Mitochondria</searchLink><br /><searchLink fieldCode="DE" term="%22Protein+Isoforms+--+chemistry%22">Protein Isoforms -- chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Porins+--+isolation+%26+purification%22">Porins -- isolation & purification</searchLink><br /><searchLink fieldCode="DE" term="%22Seeds%22">Seeds</searchLink><br /><searchLink fieldCode="DE" term="%22Phosphatidylcholines%22">Phosphatidylcholines</searchLink><br /><searchLink fieldCode="DE" term="%22Protein+Isoforms+--+isolation+%26+purification%22">Protein Isoforms -- isolation & purification</searchLink><br /><searchLink fieldCode="DE" term="%22Fabaceae+--+metabolism%22">Fabaceae -- metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22Seeds+--+chemistry%22">Seeds -- chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Sequence+Analysis%22">Sequence Analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Sequence+Alignment%22">Sequence Alignment</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+Proteins+--+chemistry%22">Plant Proteins -- chemistry</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Mitochondria were isolated from imbibed seeds of lentil (Lens culinaris) and Phaseolus vulgaris. We copurified two voltage-dependent anion channel from detergent solubilized mitochondria in a single purification step using hydroxyapatite. The two isoforms from P. vulgaris were separated by chromatofocusing chromatography in 4m urea without any loss of channel activity. Channel activity of each isoform was characterized upon reconstitution into diphytanoyl phosphatidylcholine planar lipid bilayers. Both isoforms form large conductance channels that are slightly anion selective and display cation selective substates.
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  Data: 1532-2548<br />0032-0889
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  Data: 10.1104/pp.124.3.1181
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  Data: edsair.doi.dedup.....bb6b72e0ac1e83423b74f0e52f5d209c
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        Value: 10.1104/pp.124.3.1181
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      – Text: English
    PhysicalDescription:
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        StartPage: 1181
    Subjects:
      – SubjectFull: Fabaceae -- ultrastructure
        Type: general
      – SubjectFull: 0301 basic medicine
        Type: general
      – SubjectFull: Seeds -- metabolism
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      – SubjectFull: Lipid Bilayers
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      – SubjectFull: Ion Channels
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        Type: general
      – SubjectFull: Phosphatidylcholines -- chemistry
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      – SubjectFull: Plant Proteins -- isolation & purification
        Type: general
      – SubjectFull: Protein Isoforms
        Type: general
      – SubjectFull: Voltage-Dependent Anion Channels
        Type: general
      – SubjectFull: Amino Acid Sequence
        Type: general
      – SubjectFull: Plant Proteins
        Type: general
      – SubjectFull: 0303 health sciences
        Type: general
      – SubjectFull: Porins -- chemistry
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      – SubjectFull: Plants, Medicinal
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      – SubjectFull: Ion Channels -- isolation & purification
        Type: general
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      – SubjectFull: Fabaceae
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        Type: general
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      – SubjectFull: Sequence Analysis
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      – SubjectFull: Sequence Alignment
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      – SubjectFull: Plant Proteins -- chemistry
        Type: general
    Titles:
      – TitleFull: Purification and Characterization of Two Voltage-Dependent Anion Channel Isoforms from Plant Seeds
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