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Genome-Wide Analysis of Moniliophthora roreri Facilitates the Development of Species-Specific Primers for Biomonitoring Frosty Pod Rot of Cacao

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Názov: Genome-Wide Analysis of Moniliophthora roreri Facilitates the Development of Species-Specific Primers for Biomonitoring Frosty Pod Rot of Cacao
Autori: Sara Pereira Menezes Reis, Vanessa Santana Diorato, Almiro Neves dos Santos Júnior, Ceslaine Santos Barbosa, Maisa Ciampi-Guillardi, Edson Mario de Andrade, Karina Zoila Solis, Maria Julia Silva Manco, Franciely Santos França, Danilo Vera, Arturo Ivan Garzón Catota, Givaldo Rocha Niella, Enrique Arévalo-Gardini, José Luis Solís Bonilla, Fabienne Micheli, Julio Alfonso Chia Wong, Karina Peres Gramacho
Prispievatelia: Cirad, Agritrop
Zdroj: PhytoFrontiers
Informácie o vydavateľovi: Scientific Societies, 2025.
Rok vydania: 2025
Predmety: witches' broom, [SDV.SA.STA] Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of agriculture, diagnostic, Moniliophthora, comparative genomics, champignon pathogène, biologie moléculaire, maladie fongique, [SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics, molecular diagnosis, Theobroma cacao, frosty pod rot, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, adn, maladie des plantes, variation génétique, PCR, biomonitoring, cacao pathogens, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], identification, agent pathogène
Popis: Detecting pathogens in asymptomatic plants allows for a rapid response to emerging threats, such as the introduction of new pathogens or the emergence of new resistant strains. Frosty pod rot ( Moniliophthora roreri) significantly jeopardizes cacao production by impairing pod quality and yield. However, early diagnosis is challenging due to its prolonged incubation period, compounded by symptoms resembling those of M. perniciosa, the causative agent of witches’ broom disease. To address this, a specific and sensitive quantitative real-time PCR (qPCR) assay was developed for M. roreri detection. This involved testing diagnostic primers against a panel of 252 DNA samples collected from target and nontarget species of over 13 fungal species commonly found in cacao plantations from various regions. Species-specific primer pairs were obtained through genomic comparison of Moniliophthora spp. genomes. Two primer sets, Mr77F/R and Mr78F/R, were validated using a set of M. roreri DNA samples representative of different genetic groups (Ecuador, Peru, and Mexico) and M. perniciosa, from which no amplification was detected. The limit of detection (LOD) was determined to be 0.9 ng/µl for both primers Mr77F/R and Mr78F/R. Using this set of primers, the amplification of M. roreri DNA from naturally diseased cocoa pods at various stages of infection was also successful. At the practical level, the data provided here confirm the value of molecular diagnostic testing for the early detection of M. roreri. These features are desirable for improving fungal diagnostic capacity and assisting in devising strategies to avoid pathogen dispersion within cacao-growing regions. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Druh dokumentu: Article
Popis súboru: text; application/pdf
Jazyk: English
ISSN: 2690-5442
DOI: 10.1094/phytofr-03-24-0032-r
Prístupová URL adresa: http://agritrop.cirad.fr/611599/
https://hal.science/hal-05182657v1
https://hal.science/hal-05182657v1/document
https://doi.org/10.1094/phytofr-03-24-0032-r
Rights: CC BY NC ND
Prístupové číslo: edsair.doi.dedup.....bab1d22fd1f85b4d9375e77a49ab55b5
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  Label: Title
  Group: Ti
  Data: Genome-Wide Analysis of Moniliophthora roreri Facilitates the Development of Species-Specific Primers for Biomonitoring Frosty Pod Rot of Cacao
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  Data: <searchLink fieldCode="AR" term="%22Sara+Pereira+Menezes+Reis%22">Sara Pereira Menezes Reis</searchLink><br /><searchLink fieldCode="AR" term="%22Vanessa+Santana+Diorato%22">Vanessa Santana Diorato</searchLink><br /><searchLink fieldCode="AR" term="%22Almiro+Neves+dos+Santos+Júnior%22">Almiro Neves dos Santos Júnior</searchLink><br /><searchLink fieldCode="AR" term="%22Ceslaine+Santos+Barbosa%22">Ceslaine Santos Barbosa</searchLink><br /><searchLink fieldCode="AR" term="%22Maisa+Ciampi-Guillardi%22">Maisa Ciampi-Guillardi</searchLink><br /><searchLink fieldCode="AR" term="%22Edson+Mario+de+Andrade%22">Edson Mario de Andrade</searchLink><br /><searchLink fieldCode="AR" term="%22Karina+Zoila+Solis%22">Karina Zoila Solis</searchLink><br /><searchLink fieldCode="AR" term="%22Maria+Julia+Silva+Manco%22">Maria Julia Silva Manco</searchLink><br /><searchLink fieldCode="AR" term="%22Franciely+Santos+França%22">Franciely Santos França</searchLink><br /><searchLink fieldCode="AR" term="%22Danilo+Vera%22">Danilo Vera</searchLink><br /><searchLink fieldCode="AR" term="%22Arturo+Ivan+Garzón+Catota%22">Arturo Ivan Garzón Catota</searchLink><br /><searchLink fieldCode="AR" term="%22Givaldo+Rocha+Niella%22">Givaldo Rocha Niella</searchLink><br /><searchLink fieldCode="AR" term="%22Enrique+Arévalo-Gardini%22">Enrique Arévalo-Gardini</searchLink><br /><searchLink fieldCode="AR" term="%22José+Luis+Solís+Bonilla%22">José Luis Solís Bonilla</searchLink><br /><searchLink fieldCode="AR" term="%22Fabienne+Micheli%22">Fabienne Micheli</searchLink><br /><searchLink fieldCode="AR" term="%22Julio+Alfonso+Chia+Wong%22">Julio Alfonso Chia Wong</searchLink><br /><searchLink fieldCode="AR" term="%22Karina+Peres+Gramacho%22">Karina Peres Gramacho</searchLink>
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  Data: <searchLink fieldCode="DE" term="%22witches'+broom%22">witches' broom</searchLink><br /><searchLink fieldCode="DE" term="%22[SDV%2ESA%2ESTA]+Life+Sciences+[q-bio]%2FAgricultural+sciences%2FSciences+and+technics+of+agriculture%22">[SDV.SA.STA] Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of agriculture</searchLink><br /><searchLink fieldCode="DE" term="%22diagnostic%22">diagnostic</searchLink><br /><searchLink fieldCode="DE" term="%22Moniliophthora%22">Moniliophthora</searchLink><br /><searchLink fieldCode="DE" term="%22comparative+genomics%22">comparative genomics</searchLink><br /><searchLink fieldCode="DE" term="%22champignon+pathogène%22">champignon pathogène</searchLink><br /><searchLink fieldCode="DE" term="%22biologie+moléculaire%22">biologie moléculaire</searchLink><br /><searchLink fieldCode="DE" term="%22maladie+fongique%22">maladie fongique</searchLink><br /><searchLink fieldCode="DE" term="%22[SDV%2EGEN%2EGPL]+Life+Sciences+[q-bio]%2FGenetics%2FPlants+genetics%22">[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics</searchLink><br /><searchLink fieldCode="DE" term="%22molecular+diagnosis%22">molecular diagnosis</searchLink><br /><searchLink fieldCode="DE" term="%22Theobroma+cacao%22">Theobroma cacao</searchLink><br /><searchLink fieldCode="DE" term="%22frosty+pod+rot%22">frosty pod rot</searchLink><br /><searchLink fieldCode="DE" term="%22[SDV%2EMP]+Life+Sciences+[q-bio]%2FMicrobiology+and+Parasitology%22">[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology</searchLink><br /><searchLink fieldCode="DE" term="%22[SDV%2EBV%2EPEP]+Life+Sciences+[q-bio]%2FVegetal+Biology%2FPhytopathology+and+phytopharmacy%22">[SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy</searchLink><br /><searchLink fieldCode="DE" term="%22adn%22">adn</searchLink><br /><searchLink fieldCode="DE" term="%22maladie+des+plantes%22">maladie des plantes</searchLink><br /><searchLink fieldCode="DE" term="%22variation+génétique%22">variation génétique</searchLink><br /><searchLink fieldCode="DE" term="%22PCR%22">PCR</searchLink><br /><searchLink fieldCode="DE" term="%22biomonitoring%22">biomonitoring</searchLink><br /><searchLink fieldCode="DE" term="%22cacao+pathogens%22">cacao pathogens</searchLink><br /><searchLink fieldCode="DE" term="%22[SDV%2EBBM%2EGTP]+Life+Sciences+[q-bio]%2FBiochemistry%2C+Molecular+Biology%2FGenomics+[q-bio%2EGN]%22">[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]</searchLink><br /><searchLink fieldCode="DE" term="%22identification%22">identification</searchLink><br /><searchLink fieldCode="DE" term="%22agent+pathogène%22">agent pathogène</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Detecting pathogens in asymptomatic plants allows for a rapid response to emerging threats, such as the introduction of new pathogens or the emergence of new resistant strains. Frosty pod rot ( Moniliophthora roreri) significantly jeopardizes cacao production by impairing pod quality and yield. However, early diagnosis is challenging due to its prolonged incubation period, compounded by symptoms resembling those of M. perniciosa, the causative agent of witches’ broom disease. To address this, a specific and sensitive quantitative real-time PCR (qPCR) assay was developed for M. roreri detection. This involved testing diagnostic primers against a panel of 252 DNA samples collected from target and nontarget species of over 13 fungal species commonly found in cacao plantations from various regions. Species-specific primer pairs were obtained through genomic comparison of Moniliophthora spp. genomes. Two primer sets, Mr77F/R and Mr78F/R, were validated using a set of M. roreri DNA samples representative of different genetic groups (Ecuador, Peru, and Mexico) and M. perniciosa, from which no amplification was detected. The limit of detection (LOD) was determined to be 0.9 ng/µl for both primers Mr77F/R and Mr78F/R. Using this set of primers, the amplification of M. roreri DNA from naturally diseased cocoa pods at various stages of infection was also successful. At the practical level, the data provided here confirm the value of molecular diagnostic testing for the early detection of M. roreri. These features are desirable for improving fungal diagnostic capacity and assisting in devising strategies to avoid pathogen dispersion within cacao-growing regions. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
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  Data: 10.1094/phytofr-03-24-0032-r
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        Value: 10.1094/phytofr-03-24-0032-r
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      – Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 11
        StartPage: 311
    Subjects:
      – SubjectFull: witches' broom
        Type: general
      – SubjectFull: [SDV.SA.STA] Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of agriculture
        Type: general
      – SubjectFull: diagnostic
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      – SubjectFull: Moniliophthora
        Type: general
      – SubjectFull: comparative genomics
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      – SubjectFull: Theobroma cacao
        Type: general
      – SubjectFull: frosty pod rot
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      – TitleFull: Genome-Wide Analysis of Moniliophthora roreri Facilitates the Development of Species-Specific Primers for Biomonitoring Frosty Pod Rot of Cacao
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