Zobraziť v EDS

fISHing with immunohistochemistry for housekeeping gene changes in Alzheimer’s disease using an automated quantitative analysis workflow

Uložené v:
Podrobná bibliografia
Názov: fISHing with immunohistochemistry for housekeeping gene changes in Alzheimer’s disease using an automated quantitative analysis workflow
Autori: Highet, Blake, Vikas Anekal, Praju, Ryan, Brigid, Murray, Helen, Coppieters't Wallant, Natacha, Victor Dieriks, Birger, Singh-Bains, Malvindar K, Mehrabi, Nasim F, Faull, Richard L M, Dragunow, Michael, Curtis, Maurice A
Prispievatelia: Brain Research New Zealand, Neurological Foundation of New Zealand, Health Research Council of New Zealand
Zdroj: Journal of Neurochemistry. 157:1270-1283
Informácie o vydavateľovi: Wiley, 2021.
Rok vydania: 2021
Predmety: Male, 0301 basic medicine, Messenger, Cyclophilins/analysis, Alzheimer's Disease, Biochemistry, Workflow, Cyclophilins, Essential, Immunohistochemistry/methods, 80 and over, High-Throughput Screening Assays/methods, Human health sciences, In Situ Hybridization, In Situ Hybridization, Fluorescence, Aged, 80 and over, 0303 health sciences, Genes, Essential, RNA, Messenger/analysis, In Situ Hybridization, Fluorescence/methods, DNA-Directed RNA Polymerases, Middle Aged, Immunohistochemistry, 3. Good health, Neurology, immunohistochemistry, Female, cyclophilin B, housekeeping genes, POLR2A RNA polymerase, human, automated analysis workflow, Sciences de la santé humaine, Fluorescence, Cellular and Molecular Neuroscience, 03 medical and health sciences, ubiquitin C (UBC), Alzheimer Disease, Neurologie, Humans, RNA, Messenger, DNA-Directed RNA Polymerases/analysis, Aged, Gene Expression Profiling, Ubiquitin C/analysis, High-Throughput Screening Assays, Genes, RNA, Ubiquitin C, in situ hybridization, Gene Expression Profiling/methods, Transcriptome
Popis: In situ hybridization (ISH) is a powerful tool that can be used to localize mRNA expression in tissue samples. Combining ISH with immunohistochemistry (IHC) to determine cell type provides cellular context of mRNA expression, which cannot be achieved with gene microarray or polymerase chain reaction. To study mRNA and protein expression on the same section we investigated the use of RNAscope® ISH in combination with fluorescent IHC on paraffin‐embedded human brain tissue. We first developed a high‐throughput, automated image analysis workflow for quantifying RNA puncta across the total cell population and within neurons identified by NeuN+ immunoreactivity. We then applied this automated analysis to tissue microarray (TMA) sections of middle temporal gyrus tissue (MTG) from neurologically normal and Alzheimer's Disease (AD) cases to determine the suitability of three commonly used housekeeping genes: ubiquitin C (UBC), peptidyl‐prolyl cis‐trans isomerase B (PPIB) and DNA‐directed RNA polymerase II subunit RPB1 (POLR2A). Overall, we saw a significant decrease in total and neuronal UBC expression in AD cases compared to normal cases. Total expression results were validated with RT‐qPCR using fresh frozen tissue from 5 normal and 5 AD cases. We conclude that this technique combined with our novel automated analysis pipeline provides a suitable platform to study changes in gene expression in diseased human brain tissue with cellular and anatomical context. Furthermore, our results suggest that UBC is not a suitable housekeeping gene in the study of post‐mortem AD brain tissue. image
Druh dokumentu: Article
Popis súboru: Print-Electronic; application/pdf
Jazyk: English
ISSN: 1471-4159
0022-3042
DOI: 10.1111/jnc.15283
Prístupová URL adresa: https://pubmed.ncbi.nlm.nih.gov/33368239
https://www.ncbi.nlm.nih.gov/pubmed/33368239
http://www.ncbi.nlm.nih.gov/pubmed/33368239
https://pubmed.ncbi.nlm.nih.gov/33368239/
https://onlinelibrary.wiley.com/doi/10.1111/jnc.15283
Rights: Wiley Online Library User Agreement
Prístupové číslo: edsair.doi.dedup.....b0c56a19e000b424da90b1389a82c7cf
Databáza: OpenAIRE
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://explore.openaire.eu/search/publication?articleId=doi_dedup___%3A%3Ab0c56a19e000b424da90b1389a82c7cf
    Name: EDS - OpenAIRE (s4221598)
    Category: fullText
    Text: View record at OpenAIRE
  – Url: https://resolver.ebscohost.com/openurl?sid=EBSCO:edsair&genre=article&issn=14714159&ISBN=&volume=157&issue=&date=20210202&spage=1270&pages=1270-1283&title=Journal of Neurochemistry&atitle=fISHing%20with%20immunohistochemistry%20for%20housekeeping%20gene%20changes%20in%20Alzheimer%E2%80%99s%20disease%20using%20an%20automated%20quantitative%20analysis%20workflow&aulast=Highet%2C%20Blake&id=DOI:10.1111/jnc.15283
    Name: Full Text Finder
    Category: fullText
    Text: Full Text Finder
    Icon: https://imageserver.ebscohost.com/branding/images/FTF.gif
    MouseOverText: Full Text Finder
  – Url: https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=EBSCO&SrcAuth=EBSCO&DestApp=WOS&ServiceName=TransferToWoS&DestLinkType=GeneralSearchSummary&Func=Links&author=Highet%20B
    Name: ISI
    Category: fullText
    Text: Nájsť tento článok vo Web of Science
    Icon: https://imagesrvr.epnet.com/ls/20docs.gif
    MouseOverText: Nájsť tento článok vo Web of Science
Header DbId: edsair
DbLabel: OpenAIRE
An: edsair.doi.dedup.....b0c56a19e000b424da90b1389a82c7cf
RelevancyScore: 904
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 904.015014648438
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: fISHing with immunohistochemistry for housekeeping gene changes in Alzheimer’s disease using an automated quantitative analysis workflow
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Highet%2C+Blake%22">Highet, Blake</searchLink><br /><searchLink fieldCode="AR" term="%22Vikas+Anekal%2C+Praju%22">Vikas Anekal, Praju</searchLink><br /><searchLink fieldCode="AR" term="%22Ryan%2C+Brigid%22">Ryan, Brigid</searchLink><br /><searchLink fieldCode="AR" term="%22Murray%2C+Helen%22">Murray, Helen</searchLink><br /><searchLink fieldCode="AR" term="%22Coppieters't+Wallant%2C+Natacha%22">Coppieters't Wallant, Natacha</searchLink><br /><searchLink fieldCode="AR" term="%22Victor+Dieriks%2C+Birger%22">Victor Dieriks, Birger</searchLink><br /><searchLink fieldCode="AR" term="%22Singh-Bains%2C+Malvindar+K%22">Singh-Bains, Malvindar K</searchLink><br /><searchLink fieldCode="AR" term="%22Mehrabi%2C+Nasim+F%22">Mehrabi, Nasim F</searchLink><br /><searchLink fieldCode="AR" term="%22Faull%2C+Richard+L+M%22">Faull, Richard L M</searchLink><br /><searchLink fieldCode="AR" term="%22Dragunow%2C+Michael%22">Dragunow, Michael</searchLink><br /><searchLink fieldCode="AR" term="%22Curtis%2C+Maurice+A%22">Curtis, Maurice A</searchLink>
– Name: Author
  Label: Contributors
  Group: Au
  Data: Brain Research New Zealand<br />Neurological Foundation of New Zealand<br />Health Research Council of New Zealand
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <i>Journal of Neurochemistry</i>. 157:1270-1283
– Name: Publisher
  Label: Publisher Information
  Group: PubInfo
  Data: Wiley, 2021.
– Name: DatePubCY
  Label: Publication Year
  Group: Date
  Data: 2021
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Male%22">Male</searchLink><br /><searchLink fieldCode="DE" term="%220301+basic+medicine%22">0301 basic medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Messenger%22">Messenger</searchLink><br /><searchLink fieldCode="DE" term="%22Cyclophilins%2Fanalysis%22">Cyclophilins/analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Alzheimer's+Disease%22">Alzheimer's Disease</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemistry%22">Biochemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Workflow%22">Workflow</searchLink><br /><searchLink fieldCode="DE" term="%22Cyclophilins%22">Cyclophilins</searchLink><br /><searchLink fieldCode="DE" term="%22Essential%22">Essential</searchLink><br /><searchLink fieldCode="DE" term="%22Immunohistochemistry%2Fmethods%22">Immunohistochemistry/methods</searchLink><br /><searchLink fieldCode="DE" term="%2280+and+over%22">80 and over</searchLink><br /><searchLink fieldCode="DE" term="%22High-Throughput+Screening+Assays%2Fmethods%22">High-Throughput Screening Assays/methods</searchLink><br /><searchLink fieldCode="DE" term="%22Human+health+sciences%22">Human health sciences</searchLink><br /><searchLink fieldCode="DE" term="%22In+Situ+Hybridization%22">In Situ Hybridization</searchLink><br /><searchLink fieldCode="DE" term="%22In+Situ+Hybridization%2C+Fluorescence%22">In Situ Hybridization, Fluorescence</searchLink><br /><searchLink fieldCode="DE" term="%22Aged%2C+80+and+over%22">Aged, 80 and over</searchLink><br /><searchLink fieldCode="DE" term="%220303+health+sciences%22">0303 health sciences</searchLink><br /><searchLink fieldCode="DE" term="%22Genes%2C+Essential%22">Genes, Essential</searchLink><br /><searchLink fieldCode="DE" term="%22RNA%2C+Messenger%2Fanalysis%22">RNA, Messenger/analysis</searchLink><br /><searchLink fieldCode="DE" term="%22In+Situ+Hybridization%2C+Fluorescence%2Fmethods%22">In Situ Hybridization, Fluorescence/methods</searchLink><br /><searchLink fieldCode="DE" term="%22DNA-Directed+RNA+Polymerases%22">DNA-Directed RNA Polymerases</searchLink><br /><searchLink fieldCode="DE" term="%22Middle+Aged%22">Middle Aged</searchLink><br /><searchLink fieldCode="DE" term="%22Immunohistochemistry%22">Immunohistochemistry</searchLink><br /><searchLink fieldCode="DE" term="%223%2E+Good+health%22">3. Good health</searchLink><br /><searchLink fieldCode="DE" term="%22Neurology%22">Neurology</searchLink><br /><searchLink fieldCode="DE" term="%22immunohistochemistry%22">immunohistochemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Female%22">Female</searchLink><br /><searchLink fieldCode="DE" term="%22cyclophilin+B%22">cyclophilin B</searchLink><br /><searchLink fieldCode="DE" term="%22housekeeping+genes%22">housekeeping genes</searchLink><br /><searchLink fieldCode="DE" term="%22POLR2A+RNA+polymerase%2C+human%22">POLR2A RNA polymerase, human</searchLink><br /><searchLink fieldCode="DE" term="%22automated+analysis+workflow%22">automated analysis workflow</searchLink><br /><searchLink fieldCode="DE" term="%22Sciences+de+la+santé+humaine%22">Sciences de la santé humaine</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorescence%22">Fluorescence</searchLink><br /><searchLink fieldCode="DE" term="%22Cellular+and+Molecular+Neuroscience%22">Cellular and Molecular Neuroscience</searchLink><br /><searchLink fieldCode="DE" term="%2203+medical+and+health+sciences%22">03 medical and health sciences</searchLink><br /><searchLink fieldCode="DE" term="%22ubiquitin+C+%28UBC%29%22">ubiquitin C (UBC)</searchLink><br /><searchLink fieldCode="DE" term="%22Alzheimer+Disease%22">Alzheimer Disease</searchLink><br /><searchLink fieldCode="DE" term="%22Neurologie%22">Neurologie</searchLink><br /><searchLink fieldCode="DE" term="%22Humans%22">Humans</searchLink><br /><searchLink fieldCode="DE" term="%22RNA%2C+Messenger%22">RNA, Messenger</searchLink><br /><searchLink fieldCode="DE" term="%22DNA-Directed+RNA+Polymerases%2Fanalysis%22">DNA-Directed RNA Polymerases/analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Aged%22">Aged</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+Expression+Profiling%22">Gene Expression Profiling</searchLink><br /><searchLink fieldCode="DE" term="%22Ubiquitin+C%2Fanalysis%22">Ubiquitin C/analysis</searchLink><br /><searchLink fieldCode="DE" term="%22High-Throughput+Screening+Assays%22">High-Throughput Screening Assays</searchLink><br /><searchLink fieldCode="DE" term="%22Genes%22">Genes</searchLink><br /><searchLink fieldCode="DE" term="%22RNA%22">RNA</searchLink><br /><searchLink fieldCode="DE" term="%22Ubiquitin+C%22">Ubiquitin C</searchLink><br /><searchLink fieldCode="DE" term="%22in+situ+hybridization%22">in situ hybridization</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+Expression+Profiling%2Fmethods%22">Gene Expression Profiling/methods</searchLink><br /><searchLink fieldCode="DE" term="%22Transcriptome%22">Transcriptome</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: In situ hybridization (ISH) is a powerful tool that can be used to localize mRNA expression in tissue samples. Combining ISH with immunohistochemistry (IHC) to determine cell type provides cellular context of mRNA expression, which cannot be achieved with gene microarray or polymerase chain reaction. To study mRNA and protein expression on the same section we investigated the use of RNAscope® ISH in combination with fluorescent IHC on paraffin‐embedded human brain tissue. We first developed a high‐throughput, automated image analysis workflow for quantifying RNA puncta across the total cell population and within neurons identified by NeuN+ immunoreactivity. We then applied this automated analysis to tissue microarray (TMA) sections of middle temporal gyrus tissue (MTG) from neurologically normal and Alzheimer's Disease (AD) cases to determine the suitability of three commonly used housekeeping genes: ubiquitin C (UBC), peptidyl‐prolyl cis‐trans isomerase B (PPIB) and DNA‐directed RNA polymerase II subunit RPB1 (POLR2A). Overall, we saw a significant decrease in total and neuronal UBC expression in AD cases compared to normal cases. Total expression results were validated with RT‐qPCR using fresh frozen tissue from 5 normal and 5 AD cases. We conclude that this technique combined with our novel automated analysis pipeline provides a suitable platform to study changes in gene expression in diseased human brain tissue with cellular and anatomical context. Furthermore, our results suggest that UBC is not a suitable housekeeping gene in the study of post‐mortem AD brain tissue. image
– Name: TypeDocument
  Label: Document Type
  Group: TypDoc
  Data: Article
– Name: Format
  Label: File Description
  Group: SrcInfo
  Data: Print-Electronic; application/pdf
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: ISSN
  Label: ISSN
  Group: ISSN
  Data: 1471-4159<br />0022-3042
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1111/jnc.15283
– Name: URL
  Label: Access URL
  Group: URL
  Data: <link linkTarget="URL" linkTerm="https://pubmed.ncbi.nlm.nih.gov/33368239" linkWindow="_blank">https://pubmed.ncbi.nlm.nih.gov/33368239</link><br /><link linkTarget="URL" linkTerm="https://www.ncbi.nlm.nih.gov/pubmed/33368239" linkWindow="_blank">https://www.ncbi.nlm.nih.gov/pubmed/33368239</link><br /><link linkTarget="URL" linkTerm="http://www.ncbi.nlm.nih.gov/pubmed/33368239" linkWindow="_blank">http://www.ncbi.nlm.nih.gov/pubmed/33368239</link><br /><link linkTarget="URL" linkTerm="https://pubmed.ncbi.nlm.nih.gov/33368239/" linkWindow="_blank">https://pubmed.ncbi.nlm.nih.gov/33368239/</link><br /><link linkTarget="URL" linkTerm="https://onlinelibrary.wiley.com/doi/10.1111/jnc.15283" linkWindow="_blank">https://onlinelibrary.wiley.com/doi/10.1111/jnc.15283</link>
– Name: Copyright
  Label: Rights
  Group: Cpyrght
  Data: Wiley Online Library User Agreement
– Name: AN
  Label: Accession Number
  Group: ID
  Data: edsair.doi.dedup.....b0c56a19e000b424da90b1389a82c7cf
PLink https://erproxy.cvtisr.sk/sfx/access?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsair&AN=edsair.doi.dedup.....b0c56a19e000b424da90b1389a82c7cf
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1111/jnc.15283
    Languages:
      – Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 14
        StartPage: 1270
    Subjects:
      – SubjectFull: Male
        Type: general
      – SubjectFull: 0301 basic medicine
        Type: general
      – SubjectFull: Messenger
        Type: general
      – SubjectFull: Cyclophilins/analysis
        Type: general
      – SubjectFull: Alzheimer's Disease
        Type: general
      – SubjectFull: Biochemistry
        Type: general
      – SubjectFull: Workflow
        Type: general
      – SubjectFull: Cyclophilins
        Type: general
      – SubjectFull: Essential
        Type: general
      – SubjectFull: Immunohistochemistry/methods
        Type: general
      – SubjectFull: 80 and over
        Type: general
      – SubjectFull: High-Throughput Screening Assays/methods
        Type: general
      – SubjectFull: Human health sciences
        Type: general
      – SubjectFull: In Situ Hybridization
        Type: general
      – SubjectFull: In Situ Hybridization, Fluorescence
        Type: general
      – SubjectFull: Aged, 80 and over
        Type: general
      – SubjectFull: 0303 health sciences
        Type: general
      – SubjectFull: Genes, Essential
        Type: general
      – SubjectFull: RNA, Messenger/analysis
        Type: general
      – SubjectFull: In Situ Hybridization, Fluorescence/methods
        Type: general
      – SubjectFull: DNA-Directed RNA Polymerases
        Type: general
      – SubjectFull: Middle Aged
        Type: general
      – SubjectFull: Immunohistochemistry
        Type: general
      – SubjectFull: 3. Good health
        Type: general
      – SubjectFull: Neurology
        Type: general
      – SubjectFull: immunohistochemistry
        Type: general
      – SubjectFull: Female
        Type: general
      – SubjectFull: cyclophilin B
        Type: general
      – SubjectFull: housekeeping genes
        Type: general
      – SubjectFull: POLR2A RNA polymerase, human
        Type: general
      – SubjectFull: automated analysis workflow
        Type: general
      – SubjectFull: Sciences de la santé humaine
        Type: general
      – SubjectFull: Fluorescence
        Type: general
      – SubjectFull: Cellular and Molecular Neuroscience
        Type: general
      – SubjectFull: 03 medical and health sciences
        Type: general
      – SubjectFull: ubiquitin C (UBC)
        Type: general
      – SubjectFull: Alzheimer Disease
        Type: general
      – SubjectFull: Neurologie
        Type: general
      – SubjectFull: Humans
        Type: general
      – SubjectFull: RNA, Messenger
        Type: general
      – SubjectFull: DNA-Directed RNA Polymerases/analysis
        Type: general
      – SubjectFull: Aged
        Type: general
      – SubjectFull: Gene Expression Profiling
        Type: general
      – SubjectFull: Ubiquitin C/analysis
        Type: general
      – SubjectFull: High-Throughput Screening Assays
        Type: general
      – SubjectFull: Genes
        Type: general
      – SubjectFull: RNA
        Type: general
      – SubjectFull: Ubiquitin C
        Type: general
      – SubjectFull: in situ hybridization
        Type: general
      – SubjectFull: Gene Expression Profiling/methods
        Type: general
      – SubjectFull: Transcriptome
        Type: general
    Titles:
      – TitleFull: fISHing with immunohistochemistry for housekeeping gene changes in Alzheimer’s disease using an automated quantitative analysis workflow
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Highet, Blake
      – PersonEntity:
          Name:
            NameFull: Vikas Anekal, Praju
      – PersonEntity:
          Name:
            NameFull: Ryan, Brigid
      – PersonEntity:
          Name:
            NameFull: Murray, Helen
      – PersonEntity:
          Name:
            NameFull: Coppieters't Wallant, Natacha
      – PersonEntity:
          Name:
            NameFull: Victor Dieriks, Birger
      – PersonEntity:
          Name:
            NameFull: Singh-Bains, Malvindar K
      – PersonEntity:
          Name:
            NameFull: Mehrabi, Nasim F
      – PersonEntity:
          Name:
            NameFull: Faull, Richard L M
      – PersonEntity:
          Name:
            NameFull: Dragunow, Michael
      – PersonEntity:
          Name:
            NameFull: Curtis, Maurice A
      – PersonEntity:
          Name:
            NameFull: Brain Research New Zealand
      – PersonEntity:
          Name:
            NameFull: Neurological Foundation of New Zealand
      – PersonEntity:
          Name:
            NameFull: Health Research Council of New Zealand
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 02
              M: 02
              Type: published
              Y: 2021
          Identifiers:
            – Type: issn-print
              Value: 14714159
            – Type: issn-print
              Value: 00223042
            – Type: issn-locals
              Value: edsair
          Numbering:
            – Type: volume
              Value: 157
          Titles:
            – TitleFull: Journal of Neurochemistry
              Type: main
ResultId 1