Simvastatin induces adverse effects on proliferation and mineralization of human primary osteoblasts
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| Název: | Simvastatin induces adverse effects on proliferation and mineralization of human primary osteoblasts |
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| Autoři: | Sabandal, Martin M. I., Schäfer, Edgar, Aed, Jonathan, Jung, Susanne, Kleinheinz, Johannes, Sielker, Sonja |
| Přispěvatelé: | Universitäts- und Landesbibliothek Münster |
| Zdroj: | Head Face Med Head & Face Medicine, Vol 16, Iss 1, Pp 1-9 (2020) |
| Informace o vydavateli: | Springer Science and Business Media LLC, 2020. |
| Rok vydání: | 2020 |
| Témata: | Adult, Male, 0301 basic medicine, Simvastatin, Mineralization, 610 Medicine and health, Specialties of internal medicine, 03 medical and health sciences, Osteogenesis, Humans, ddc:610, Cell Proliferation, 0303 health sciences, Osteoblasts, Adverse effects, Research, Anticholesteremic Agents, Osteogenesis/drug effects [MeSH], Female [MeSH], Cell Proliferation [MeSH], Adult [MeSH], Humans [MeSH], Cell Differentiation [MeSH], Anticholesteremic Agents/adverse effects [MeSH], Simvastatin/adverse effects [MeSH], Male [MeSH], Osteoblasts/drug effects [MeSH], Cell Differentiation, 3. Good health, RC581-951, Medicine and health, Female |
| Popis: | Background Frequently statins were administered to reduce the LDL-concentration in circulating blood. Especially simvastatin (SV) is an often prescribed statin. Pleiotropic effects of these drugs were reported. Thus, the aim of this study was to evaluate effects of SV on osteoblastic mineralization. Methods After informed consent primary osteoblasts were collected from tissue surplus after treatment of 14 individuals in the Department of Cranio-Maxillofacial Surgery, University Hospital Münster. The cells were passaged according to established protocols. Viability, mineralization capability and osteoblastic marker (alkaline phosphatase) were determined at day 9, 13 and 16 after adding various SV concentrations (0.05 μM, 0.1 μM, 0.5 μM, 1.0 μM). Statistical analysis was performed using the Kruskal-Wallis-test. Results The cell cultures showed a time and dose-dependent significantly decreased viability (p ) and a significantly increased mineralization (p ) in a late mineralization stage after adding SV. The typical alteration of the alkaline phosphatase (ALP) levels during osteogenic differentiation was not recognizable. Conclusions The pleiotropic effects found for different SV concentrations were possibly originated from other mineralization pathways beside the ALP induced one. Additionally, possible alterations of protein expression levels during mineralization and investigation of possible deviating application of SV in other treatment fields can be considered after gaining a deeper insight in the affected mechanisms. |
| Druh dokumentu: | Article Conference object Other literature type |
| Popis souboru: | application/pdf |
| Jazyk: | English |
| ISSN: | 1746-160X |
| DOI: | 10.1186/s13005-020-00232-4 |
| DOI: | 10.17879/81019520868 |
| Přístupová URL adresa: | https://head-face-med.biomedcentral.com/track/pdf/10.1186/s13005-020-00232-4 https://pubmed.ncbi.nlm.nih.gov/32819403 https://doaj.org/article/c5e0da6a123e4d58a0f14394b6ee80ce https://link.springer.com/article/10.1186/s13005-020-00232-4 https://www.scilit.net/article/98c15e6d5ca85172daf282887fb5172c https://head-face-med.biomedcentral.com/articles/10.1186/s13005-020-00232-4 https://www.ncbi.nlm.nih.gov/pubmed/32819403 https://pubmed.ncbi.nlm.nih.gov/32819403/ https://link.springer.com/content/pdf/10.1186/s13005-020-00232-4.pdf https://repository.publisso.de/resource/frl:6466172 https://repositorium.uni-muenster.de/transfer/miami/24b52f47-539e-4505-a082-7247dd93f1f1 |
| Rights: | CC BY |
| Přístupové číslo: | edsair.doi.dedup.....ad8c149b4d0dbc26bb3722b8d79efb21 |
| Databáze: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Background Frequently statins were administered to reduce the LDL-concentration in circulating blood. Especially simvastatin (SV) is an often prescribed statin. Pleiotropic effects of these drugs were reported. Thus, the aim of this study was to evaluate effects of SV on osteoblastic mineralization. Methods After informed consent primary osteoblasts were collected from tissue surplus after treatment of 14 individuals in the Department of Cranio-Maxillofacial Surgery, University Hospital Münster. The cells were passaged according to established protocols. Viability, mineralization capability and osteoblastic marker (alkaline phosphatase) were determined at day 9, 13 and 16 after adding various SV concentrations (0.05 μM, 0.1 μM, 0.5 μM, 1.0 μM). Statistical analysis was performed using the Kruskal-Wallis-test. Results The cell cultures showed a time and dose-dependent significantly decreased viability (p ) and a significantly increased mineralization (p ) in a late mineralization stage after adding SV. The typical alteration of the alkaline phosphatase (ALP) levels during osteogenic differentiation was not recognizable. Conclusions The pleiotropic effects found for different SV concentrations were possibly originated from other mineralization pathways beside the ALP induced one. Additionally, possible alterations of protein expression levels during mineralization and investigation of possible deviating application of SV in other treatment fields can be considered after gaining a deeper insight in the affected mechanisms. |
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| ISSN: | 1746160X |
| DOI: | 10.1186/s13005-020-00232-4 |