Dietary polyunsaturated fatty acids and heme iron induce oxidative stress biomarkers and a cancer promoting environment in the colon of rats
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| Názov: | Dietary polyunsaturated fatty acids and heme iron induce oxidative stress biomarkers and a cancer promoting environment in the colon of rats |
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| Autori: | Guéraud, Françoise, Taché, Sylviane, Steghens, J. P., Milkovic, L., Borovic-Sunjic, S., Zarkovic, N., Gaultier, Eric, Naud, Nathalie, Héliès-Toussaint, Cécile, Pierre, F., Priymenko, Nathalie |
| Prispievatelia: | Guéraud, Françoise, Taché, Sylviane, Steghens, Jean-Paul, Milkovic, Lidija, Borovic-Sunjic, Suzana, Zarkovic, Neven, Pierre, Fabrice, Priymenko, Nathalie, Pillet, Lauriane, Prévention et promotion de la cancérogénèse par les aliments (ToxAlim-PPCA), ToxAlim (ToxAlim), Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Rudjer Boskovic Institute Zagreb, Endocrinologie & Toxicologie de la Barrière Intestinale (ToxAlim-ENTeRisk) |
| Zdroj: | Free Radical Biology and Medicine (83), 192-200. (2015) |
| Informácie o vydavateľovi: | Elsevier BV, 2015. |
| Rok vydania: | 2015 |
| Predmety: | 0301 basic medicine, [SDV]Life Sciences [q-bio], Colonic Neoplasms/etiology/metabolism/*pathology, Heme/*metabolism, Rectal Neoplasms/etiology/metabolism/pathology, Thiobarbituric Acid Reactive Substances/metabolism, Antioxidants, Mice, Malondialdehyde, Tumor Microenvironment, Tumor Cells, Cultured, 2. Zero hunger, Inbred F344, 0303 health sciences, Cultured, Iron/*metabolism, Fatty Acids, Colon/drug effects/metabolism/*pathology, Lipid Peroxidation/drug effects, Unsaturated/*adverse effects, 6. Clean water, Tumor Cells, 3. Good health, Oxidative Stress, [SDV] Life Sciences [q-bio], 4-Hydroxynonenal, Biomarkers, Colorectal cancer, Heme iron, Lipid peroxidation, Polyunsaturated fatty acids, Colonic Neoplasms, Fatty Acids, Unsaturated, Female, Antioxidants/metabolism, Colon, Iron, Heme, Thiobarbituric Acid Reactive Substances, 03 medical and health sciences, Reactive Oxygen Species/metabolism, Animals, Diet/*adverse effects, Rectal Neoplasms, Biomarkers/*urine, Rats, Inbred F344, Diet, Rats, Malondialdehyde/metabolism, Lipid Peroxidation, Reactive Oxygen Species |
| Popis: | The end products of polyunsaturated fatty acid (PUFA) peroxidation, such as malondialdehyde (MDA), 4-hydroxynonenal (HNE), and isoprostanes (8-iso-PGF2α), are widely used as systemic lipid oxidation/oxidative stress biomarkers. However, some of these compounds have also a dietary origin. Thus, replacing dietary saturated fat by PUFAs would improve health but could also increase the formation of such compounds, especially in the case of a pro-oxidant/antioxidant imbalanced diet. Hence, the possible impact of dietary fatty acids and pro-oxidant compounds was studied in rats given diets allowing comparison of the effects of heme iron vs. ferric citrate and of ω-6- vs. ω-3-rich oil on the level of lipid peroxidation/oxidative stress biomarkers. Rats given a heme iron-rich diet without PUFA were used as controls. The results obtained have shown that MDA and the major urinary metabolite of HNE (the mercapturic acid of dihydroxynonane, DHN-MA) were highly dependent on the dietary factors tested, while 8-iso-PGF2α was modestly but significantly affected. Intestinal inflammation and tissue fatty acid composition were checked in parallel and could only explain the differences we observed to a limited extent. Thus, the differences in biomarkers were attributed to the formation of lipid oxidation compounds in food or during digestion, their intestinal absorption, and their excretion into urine. Moreover, fecal extracts from the rats fed the heme iron or fish oil diets were highly toxic for immortalized mouse colon cells. Such toxicity can eventually lead to promotion of colorectal carcinogenesis, supporting the epidemiological findings between red meat intake and colorectal cancer risk. Therefore, the analysis of these biomarkers of lipid peroxidation/oxidative stress in urine should be used with caution when dietary factors are not well controlled, while control of their possible dietary intake is needed also because of their pro-inflammatory, toxic, and even cocarcinogenic effects. |
| Druh dokumentu: | Article |
| Popis súboru: | application/pdf |
| Jazyk: | English |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/j.freeradbiomed.2015.02.023 |
| Prístupová URL adresa: | https://hal.archives-ouvertes.fr/hal-01850558/file/Gueraud2015FRBM_1.pdf https://pubmed.ncbi.nlm.nih.gov/25744414 http://www.sciencedirect.com/science/article/pii/S0891584915000891 https://doi.org/10.1016/j.freeradbiomed.2015.02.023 https://www.sciencedirect.com/science/article/pii/S0891584915000891 http://www.ncbi.nlm.nih.gov/pubmed/25744414 https://pubmed.ncbi.nlm.nih.gov/25744414/ http://prodinra.inra.fr/record/319277 https://hal.science/hal-01850558v1/document https://hal.science/hal-01850558v1 https://doi.org/10.1016/j.freeradbiomed.2015.02.023 |
| Rights: | Elsevier TDM CC BY SA |
| Prístupové číslo: | edsair.doi.dedup.....a28f18ac5f3cf0ffd2dad14f2c6a859c |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | The end products of polyunsaturated fatty acid (PUFA) peroxidation, such as malondialdehyde (MDA), 4-hydroxynonenal (HNE), and isoprostanes (8-iso-PGF2α), are widely used as systemic lipid oxidation/oxidative stress biomarkers. However, some of these compounds have also a dietary origin. Thus, replacing dietary saturated fat by PUFAs would improve health but could also increase the formation of such compounds, especially in the case of a pro-oxidant/antioxidant imbalanced diet. Hence, the possible impact of dietary fatty acids and pro-oxidant compounds was studied in rats given diets allowing comparison of the effects of heme iron vs. ferric citrate and of ω-6- vs. ω-3-rich oil on the level of lipid peroxidation/oxidative stress biomarkers. Rats given a heme iron-rich diet without PUFA were used as controls. The results obtained have shown that MDA and the major urinary metabolite of HNE (the mercapturic acid of dihydroxynonane, DHN-MA) were highly dependent on the dietary factors tested, while 8-iso-PGF2α was modestly but significantly affected. Intestinal inflammation and tissue fatty acid composition were checked in parallel and could only explain the differences we observed to a limited extent. Thus, the differences in biomarkers were attributed to the formation of lipid oxidation compounds in food or during digestion, their intestinal absorption, and their excretion into urine. Moreover, fecal extracts from the rats fed the heme iron or fish oil diets were highly toxic for immortalized mouse colon cells. Such toxicity can eventually lead to promotion of colorectal carcinogenesis, supporting the epidemiological findings between red meat intake and colorectal cancer risk. Therefore, the analysis of these biomarkers of lipid peroxidation/oxidative stress in urine should be used with caution when dietary factors are not well controlled, while control of their possible dietary intake is needed also because of their pro-inflammatory, toxic, and even cocarcinogenic effects. |
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| ISSN: | 08915849 |
| DOI: | 10.1016/j.freeradbiomed.2015.02.023 |