Two-Dimensional Antifouling Fluidic Channels on Nanopapers for Biosensing
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| Název: | Two-Dimensional Antifouling Fluidic Channels on Nanopapers for Biosensing |
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| Autoři: | Orelma, Hannes, Borghei, Maryam, Vuoriluoto, Maija, Koivunen, Risto, Rojas, Orlando, J., Solin, Katariina |
| Přispěvatelé: | Department of Bioproducts and Biosystems, Bio-based Colloids and Materials, Printing Technology, Aalto-yliopisto, Aalto University |
| Zdroj: | Biomacromolecules |
| Informace o vydavateli: | American Chemical Society (ACS), 2018. |
| Rok vydání: | 2018 |
| Témata: | Ethylene Glycol, Polymers, Surface Properties, Nanofibers, Biosensing Techniques, 02 engineering and technology, Polyethylene Glycols, Polystyrenes/chemistry, Biosensing Techniques/methods, Polymers/chemistry, Ethylene Glycol/chemistry, Humans, ta216, Cellulose, ta215, Cellulose/chemistry, Serum Albumin, Fibrinogen/chemistry, ta1182, Nanofibers/chemistry, Fibrinogen, Bovine/chemistry, Serum Albumin, Bovine, Surface Plasmon Resonance, Polyethylene Glycols/chemistry, Surface Plasmon Resonance/methods, Nylons, Immunoglobulin G/chemistry, Printing/methods, Immunoglobulin G, Methacrylates, Nanoparticles, Polystyrenes, Printing, Methacrylates/chemistry, Nanoparticles/chemistry, 0210 nano-technology, Nylons/chemistry |
| Popis: | Two-dimensional (hydrophilic) channels were patterned on films prepared from cellulose nanofibrils (CNF) using photolithography and inkjet printing. Such processes included UV-activated thiol-yne click coupling and inkjet-printed designs with polystyrene. The microfluidic channels were characterized (SEM, wetting, and fluid flow) and applied as platforms for biosensing. Compared to results from the click method, a better feature fidelity and flow properties were achieved with the simpler inkjet-printed channels. Human immunoglobulin G (hIgG) was used as target protein after surface modification with either bovine serum albumin (BSA), fibrinogen, or block copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) (PDMAEMA- block-POEGMA copolymers). Surface plasmon resonance (SPR) and AFM imaging were used to determine their antifouling effect to prevent nonspecific hIgG binding. Confocal laser scanning microscopy revealed diffusion and adsorption traces in the channels. The results confirm an effective surface passivation of the microfluidic channels (95% reduction of hIgG adsorption and binding). The inexpensive and disposable systems proposed here allow designs with space-resolved blocking efficiency that offer a great potential in biosensing. |
| Druh dokumentu: | Article |
| Popis souboru: | application/pdf |
| Jazyk: | English |
| ISSN: | 1526-4602 1525-7797 |
| DOI: | 10.1021/acs.biomac.8b01656 |
| Přístupová URL adresa: | https://pubs.acs.org/doi/pdf/10.1021/acs.biomac.8b01656 https://pubmed.ncbi.nlm.nih.gov/30576124 http://juuli.fi/Record/0335323319 https://doi.org/10.1021/acs.biomac.8b01656 http://juuli.fi/Record/0338027619 https://doi.org/10.1021/acs.biomac.8b01656 https://cris.vtt.fi/en/publications/005cad27-4089-4506-bed5-9226597f6e84 https://doi.org/10.1021/acs.biomac.8b01656 https://www.ncbi.nlm.nih.gov/pubmed/30576124 https://research.aalto.fi/en/publications/two-dimensional-antifouling-fluidic-channels-on-nanopapers-for-bi https://pubs.acs.org/doi/full/10.1021/acs.biomac.8b01656 https://research.aalto.fi/files/32195846/CHEM_Solin_et_al_Two_Dimensional_Antifouling_Fluidic_2019_acs.biomac.pdf https://pubs.acs.org/doi/10.1021/acs.biomac.8b01656 https://pubag.nal.usda.gov/catalog/6305785 https://aaltodoc.aalto.fi/handle/123456789/36679 |
| Rights: | Standard ACS AuthorChoice/Editors’ Choice Usage Agreement acs-specific: authorchoice/editors choice usage agreement |
| Přístupové číslo: | edsair.doi.dedup.....a0fe6a135477b0144c609e8dd10b1e04 |
| Databáze: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstrakt: | Two-dimensional (hydrophilic) channels were patterned on films prepared from cellulose nanofibrils (CNF) using photolithography and inkjet printing. Such processes included UV-activated thiol-yne click coupling and inkjet-printed designs with polystyrene. The microfluidic channels were characterized (SEM, wetting, and fluid flow) and applied as platforms for biosensing. Compared to results from the click method, a better feature fidelity and flow properties were achieved with the simpler inkjet-printed channels. Human immunoglobulin G (hIgG) was used as target protein after surface modification with either bovine serum albumin (BSA), fibrinogen, or block copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA) (PDMAEMA- block-POEGMA copolymers). Surface plasmon resonance (SPR) and AFM imaging were used to determine their antifouling effect to prevent nonspecific hIgG binding. Confocal laser scanning microscopy revealed diffusion and adsorption traces in the channels. The results confirm an effective surface passivation of the microfluidic channels (95% reduction of hIgG adsorption and binding). The inexpensive and disposable systems proposed here allow designs with space-resolved blocking efficiency that offer a great potential in biosensing. |
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| ISSN: | 15264602 15257797 |
| DOI: | 10.1021/acs.biomac.8b01656 |