Investigation of the effect of astaxanthin on alveolar bone loss in experimental periodontitis
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| Titel: | Investigation of the effect of astaxanthin on alveolar bone loss in experimental periodontitis |
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| Autoren: | Balci Yuce, H., Lektemur Alpan, Aysan, Gevrek, F., Toker, H. |
| Weitere Verfasser: | Yuce, H. Balci Gaziosmanpasa Univ, Dept Periodontol, Fac Dent, Tokat, Turkey -- Alpan, A. Lektemur Pamukkale Univ, Dept Periodontol, Fac Dent, Denizli, Turkey -- Gevrek, F. Gaziosmanpasa Univ, Fac Med, Dept Histol & Embryol, Tokat, Turkey -- Toker, H. Cumhuriyet Univ, Dept Periodontol, Fac Dent, Sivas, Turkey, balci yuce, hatice -- 0000-0003-3574-9751, LEKTEMUR ALPAN, AYSAN -- 0000-0002-5939-4783 |
| Quelle: | Journal of Periodontal Research. 53:131-138 |
| Verlagsinformationen: | Wiley, 2017. |
| Publikationsjahr: | 2017 |
| Schlagwörter: | alveolar bone loss, antioxidant, Osteocalcin, Wistar, Alveolar Bone Loss, osteocalcin, Bone Morphogenetic Protein 2, Cell Count, bone morphogenetic protein 2, Alveolar Bone Loss/*prevention & control, Animals, Antioxidants/*pharmacology, Bone Morphogenetic Protein 2/metabolism, Disease Models, Animal, Nitric Oxide Synthase/metabolism, Osteoblasts/drug effects, Osteocalcin/metabolism, Periodontitis/*drug therapy, Rats, Wistar, Xanthophylls/pharmacology, bcl-2-Associated X Protein/metabolism, Wistar rat, Xanthophylls, Antioxidants, 03 medical and health sciences, Bax protein, rat, 0302 clinical medicine, rat, xanthophyll, animal, Periodontitis, periodontitis, cell count, experimental periodontitis, bcl-2-Associated X Protein, Osteoblasts, Animal, nitric oxide synthase, disease model, drug effect, inducible nitric oxide synthase, 16. Peace & justice, Rats, 3. Good health, astaxanthin, antioxidants, Disease Models, osteoblast, tartrate-resistant acid phosphatase, Nitric Oxide Synthase, Bax protein, metabolism, protein Bax |
| Beschreibung: | Background and ObjectiveAstaxanthin is a keto‐carotenoid that has a strong antioxidant effect. The purpose of this study was to evaluate the effects of astaxanthin on alveolar bone loss and histopathological changes in ligature‐induced periodontitis in rats.Material and methodsWistar rats were divided into four experimental groups: non‐ligated (C, n = 6); ligature only (L, n = 6); ligature and astaxanthin (1 mg/kg/day astaxanthin,AS1 group, n = 8); ligature and astaxanthin (5 mg/kg/day astaxanthin,AS5 group, n = 8). Silk ligatures were placed at the gingival margin of lower first molars of the mandibular quadrant. The study duration was 11 days and the animals were killed at the end of this period. Changes in alveolar bone levels were clinically measured and tissues were immunohistochemically examined, osteocalcin, bone morphogenic protein‐2, inducible nitric oxide synthase, Bax and bcl‐2 levels in alveolar bone and tartrate‐resistant acid phosphatase‐positive osteoclast cells, osteoblast and inflammatory cell counts were determined.ResultsAlveolar bone loss was highest in the L group and the differences among the L,AS1 andAS5 groups were also significant (P < .05). Both doses of astaxanthin decreasedtartrate‐resistant acid phosphatase‐positive+ osteoclast cell and increased osteoblast cell counts (P < .05). The inflammation in the L group was also higher than those of the C andAS1 groups were (P < .05) indicating the anti‐inflammatory effect of astaxanthin. Althoughinducible nitric oxide synthase,osteocalcin,bone morphogenic protein‐2 and bax staining percentages were all highest in theAS5 group and bcl‐2 staining percentage was highest in theAS1 group, values were close to each other (P > .05).ConclusionWithin the limits of this study, it can be suggested that astaxanthin administration may reduce alveolar bone loss by increasing osteoblastic activity and decrease osteoclastic activity in experimental periodontitis model. |
| Publikationsart: | Article |
| Sprache: | English |
| ISSN: | 1600-0765 0022-3484 |
| DOI: | 10.1111/jre.12497 |
| Zugangs-URL: | https://pubmed.ncbi.nlm.nih.gov/29044575 https://www.ncbi.nlm.nih.gov/pubmed/29044575 https://onlinelibrary.wiley.com/doi/10.1111/jre.12497/full?scrollTo=references https://onlinelibrary.wiley.com/doi/10.1111/jre.12497 https://europepmc.org/article/MED/29044575 https://hdl.handle.net/20.500.12418/6388 http://acikerisim.pau.edu.tr:8080/xmlui/handle/11499/10857 |
| Rights: | Wiley Online Library User Agreement |
| Dokumentencode: | edsair.doi.dedup.....a02340a5adc74b64e808d9c9d6ecb016 |
| Datenbank: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstract: | Background and ObjectiveAstaxanthin is a keto‐carotenoid that has a strong antioxidant effect. The purpose of this study was to evaluate the effects of astaxanthin on alveolar bone loss and histopathological changes in ligature‐induced periodontitis in rats.Material and methodsWistar rats were divided into four experimental groups: non‐ligated (C, n = 6); ligature only (L, n = 6); ligature and astaxanthin (1 mg/kg/day astaxanthin,AS1 group, n = 8); ligature and astaxanthin (5 mg/kg/day astaxanthin,AS5 group, n = 8). Silk ligatures were placed at the gingival margin of lower first molars of the mandibular quadrant. The study duration was 11 days and the animals were killed at the end of this period. Changes in alveolar bone levels were clinically measured and tissues were immunohistochemically examined, osteocalcin, bone morphogenic protein‐2, inducible nitric oxide synthase, Bax and bcl‐2 levels in alveolar bone and tartrate‐resistant acid phosphatase‐positive osteoclast cells, osteoblast and inflammatory cell counts were determined.ResultsAlveolar bone loss was highest in the L group and the differences among the L,AS1 andAS5 groups were also significant (P < .05). Both doses of astaxanthin decreasedtartrate‐resistant acid phosphatase‐positive+ osteoclast cell and increased osteoblast cell counts (P < .05). The inflammation in the L group was also higher than those of the C andAS1 groups were (P < .05) indicating the anti‐inflammatory effect of astaxanthin. Althoughinducible nitric oxide synthase,osteocalcin,bone morphogenic protein‐2 and bax staining percentages were all highest in theAS5 group and bcl‐2 staining percentage was highest in theAS1 group, values were close to each other (P > .05).ConclusionWithin the limits of this study, it can be suggested that astaxanthin administration may reduce alveolar bone loss by increasing osteoblastic activity and decrease osteoclastic activity in experimental periodontitis model. |
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| ISSN: | 16000765 00223484 |
| DOI: | 10.1111/jre.12497 |