Deficiency of α7 Cholinergic Receptors Facilitates Bacterial Clearance inEscherichia coliPeritonitis
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| Názov: | Deficiency of α7 Cholinergic Receptors Facilitates Bacterial Clearance inEscherichia coliPeritonitis |
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| Autori: | Giebelen, Ilona A J, Le Moine, Alain, van den Pangaart, Petra S, Sadis, Claude, Goldman, Michel, Florquin, Sandrine, van der Poll, Tom |
| Zdroj: | The Journal of Infectious Diseases. 198:750-757 |
| Informácie o vydavateľovi: | Oxford University Press (OUP), 2008. |
| Rok vydania: | 2008 |
| Predmety: | Male, 0301 basic medicine, Escherichia coli Infections -- microbiology, Inflammation -- immunology, alpha7 Nicotinic Acetylcholine Receptor, Neutrophils, Peritonitis, Receptors, Nicotinic, Kidney, Lung -- microbiology, Escherichia coli -- immunology, Mice, 03 medical and health sciences, Escherichia coli Infections -- immunology, Receptors, Peritoneum -- microbiology, Escherichia coli, Animals, Genetic Predisposition to Disease, Kidney -- microbiology, Lung, Escherichia coli Infections, Inflammation, Escherichia coli -- growth & development, 0303 health sciences, Spleen -- microbiology, Nicotinic -- metabolism, Peritonitis -- immunology, Sciences bio-médicales et agricoles, 3. Good health, Peritonitis -- microbiology, Gene Expression Regulation, Liver, Neutrophils -- immunology, Nicotinic -- genetics, Female, Peritoneum, Liver -- microbiology, Gene Deletion, Spleen |
| Popis: | The efferent vagus nerve can inhibit inflammation via interaction between acetylcholine and alpha7 cholinergic receptors.To determine the role played by alpha7 receptors in antibacterial defense, peritonitis was induced in alpha7 receptor-deficient (alpha7(-/-)) and wild-type (WT) mice by intraperitoneal injection with Escherichia coli.At 20 h after infection, virtually all alpha7(-/-) mice had cleared the infection from their peritoneal cavities and had sterile blood cultures, whereas WT mice had high bacterial loads at the primary site of infection and were bacteremic. In addition, bacterial burdens in liver, spleen, kidneys, and lungs were much lower in alpha7(-/-) mice, and these animals displayed a diminished inflammatory response, as reflected by a reduced number of infiltrating neutrophils in peritoneal lavage fluid and lower circulating cytokine levels. At 2 h after infection, however, when bacterial loads were still similar in alpha7(-/-) and WT mice, the former mouse strain showed a more robust influx of neutrophils into the peritoneal cavity.Deficiency of the alpha7 receptor is associated with an accelerated clearance of E. coli after intraperitoneal infection, preceded by a faster recruitment of neutrophils. These data provide the first evidence for a detrimental role of alpha7 receptors in the host defense against bacteria. |
| Druh dokumentu: | Article |
| Popis súboru: | 1 full-text file(s): application/pdf |
| Jazyk: | English |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1086/590432 |
| Prístupová URL adresa: | https://academic.oup.com/jid/article-pdf/198/5/750/18055261/198-5-750.pdf https://pubmed.ncbi.nlm.nih.gov/18627272 https://difusion.ulb.ac.be/vufind/Record/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/51087/Details https://academic.oup.com/jid/article/198/5/750/868316 https://www.ncbi.nlm.nih.gov/pubmed/18627272 https://europepmc.org/abstract/MED/18627272 https://pubmed.ncbi.nlm.nih.gov/18627272/ https://paperity.org/p/47556567/deficiency-of-a7-cholinergic-receptors-facilitates-bacterial-clearance-in-escherichia https://pure.amsterdamumc.nl/en/publications/ab12d8bf-0e5b-4dab-95ff-d883471b9459 https://doi.org/10.1086/590432 |
| Prístupové číslo: | edsair.doi.dedup.....9eb93e6ee7bfef5f59ae3dfc5a9077f7 |
| Databáza: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstrakt: | The efferent vagus nerve can inhibit inflammation via interaction between acetylcholine and alpha7 cholinergic receptors.To determine the role played by alpha7 receptors in antibacterial defense, peritonitis was induced in alpha7 receptor-deficient (alpha7(-/-)) and wild-type (WT) mice by intraperitoneal injection with Escherichia coli.At 20 h after infection, virtually all alpha7(-/-) mice had cleared the infection from their peritoneal cavities and had sterile blood cultures, whereas WT mice had high bacterial loads at the primary site of infection and were bacteremic. In addition, bacterial burdens in liver, spleen, kidneys, and lungs were much lower in alpha7(-/-) mice, and these animals displayed a diminished inflammatory response, as reflected by a reduced number of infiltrating neutrophils in peritoneal lavage fluid and lower circulating cytokine levels. At 2 h after infection, however, when bacterial loads were still similar in alpha7(-/-) and WT mice, the former mouse strain showed a more robust influx of neutrophils into the peritoneal cavity.Deficiency of the alpha7 receptor is associated with an accelerated clearance of E. coli after intraperitoneal infection, preceded by a faster recruitment of neutrophils. These data provide the first evidence for a detrimental role of alpha7 receptors in the host defense against bacteria. |
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| ISSN: | 15376613 00221899 |
| DOI: | 10.1086/590432 |