Counteraction of perforated cecum lesions in rats: effects of pentadecapeptide BPC 157, L-NAME and L-arginine
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| Názov: | Counteraction of perforated cecum lesions in rats: effects of pentadecapeptide BPC 157, L-NAME and L-arginine |
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| Autori: | Drmić, Domagoj, Samara, Mariam, Vidović, Tinka, Malekinušić, Dominik, Antunović, Marko, Vrdoljak, Borna, Ruzman, Jelena, Milković Periša, Marija, Horvat Pavlov, Katarina, Jeyakumar, Jerusha, Seiwerth, Sven, Sikirić, Predrag |
| Zdroj: | World J Gastroenterol |
| Informácie o vydavateľovi: | Baishideng Publishing Group Inc., 2018. |
| Rok vydania: | 2018 |
| Predmety: | Male, 0301 basic medicine, vessels, BPC 157, L-NAME, L-arginine, perforated cecum, rats, Drug Therapy, Combination/methods, Arginine, Nitric Oxide, Cecum/blood supply, 03 medical and health sciences, NG-Nitroarginine Methyl Ester/therapeutic use, Animals, Humans, NG-Nitroarginine Methyl Ester/pharmacology, Cecum/injuries, Rats, Wistar, Wound Healing/drug effects, Cecum, Intestinal Perforation/drug therapy, Peptide Fragments/therapeutic use, Wound Healing, 0303 health sciences, Peptide Fragments/pharmacology, Cecum/drug effects, Proteins, Basic Study, Proteins/pharmacology, Anti-Ulcer Agents, Peptide Fragments, Arginine/therapeutic use, Rats, 3. Good health, Disease Models, Animal, NG-Nitroarginine Methyl Ester, Treatment Outcome, Proteins/therapeutic use, Intestinal Perforation, Nitric Oxide/metabolism, Anti-Ulcer Agents/pharmacology, Drug Therapy, Combination, Anti-Ulcer Agents/therapeutic use, Arginine/pharmacology |
| Popis: | To study the counteraction of perforated cecum lesion using BPC 157 and nitric oxide (NO) system agents.Alongside with the agents' application (after 1 min, medication (/kg, 10 mL/2 min bath/rat) includes: BPC 157 (10 μg), L-NAME (5 mg), L-arginine (100mg) alone or combined, and saline baths (controls)) on the rat perforate cecum injury, we continuously assessed the gross reappearance of the vessels (USB microcamera) quickly propagating toward the defect at the cecum surface, defect contraction, bleeding attenuation, MDA- and NO-levels in cecum tissue at 15 min, and severity of cecum lesions and adhesions at 1 and 7 d.Post-injury, during/after a saline bath, the number of vessels was significantly reduced, the defect was slightly narrowed, bleeding was significant and MDA-levels increased and NO-levels decreased. BPC 157 bath: the vessel presentation was markedly increased, the defect was noticeably narrowed, the bleeding time was shortened and MDA- and NO-levels remained normal. L-NAME: reduced vessel presentation but not more than the control, did not change defect and shortened bleeding. L-arginine: exhibited less vessel reduction, did not change the defect and prolonged bleeding. In combination, mutual counteraction occurred (L-NAME + L-arginine) or the presentation was similar to that of BPC 157 rats (BPC 157 + L-NAME; BPC 157 + L-arginine; BPC 157 + L-NAME + L-arginine), except the defect did not change. Thereby at day 1 and 7, saline, L-NAME, L-arginine and L-NAME + L-arginine failed (defect was still open and large adhesions present).The therapeutic effect was achieved with BPC 157 alone or in combination with L-NAME and L-arginine as it was able to consolidate the stimulating and inhibiting effects of the NO-system towards more effective healing recruiting vessels. |
| Druh dokumentu: | Article Other literature type |
| Popis súboru: | application/pdf |
| ISSN: | 1007-9327 |
| DOI: | 10.3748/wjg.v24.i48.5462 |
| Prístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/30622376 https://www.bib.irb.hr/981043 https://doi.org/10.3748/wjg.v24.i48.5462 https://www.wjgnet.com/1007-9327/full/v24/i48/5462.htm https://doi.org/10.3748/wjg.v24.i48.5462 https://europepmc.org/article/MED/30622376 https://pubmed.ncbi.nlm.nih.gov/30622376/ https://www.ncbi.nlm.nih.gov/pubmed/30622376 https://www.wjgnet.com/1007-9327/full/v24/i48/5462.htm |
| Rights: | CC BY NC URL: http://rightsstatements.org/vocab/InC/1.0/ |
| Prístupové číslo: | edsair.doi.dedup.....7dcc2a351d1eea7480fb303e8b98f90c |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | To study the counteraction of perforated cecum lesion using BPC 157 and nitric oxide (NO) system agents.Alongside with the agents' application (after 1 min, medication (/kg, 10 mL/2 min bath/rat) includes: BPC 157 (10 μg), L-NAME (5 mg), L-arginine (100mg) alone or combined, and saline baths (controls)) on the rat perforate cecum injury, we continuously assessed the gross reappearance of the vessels (USB microcamera) quickly propagating toward the defect at the cecum surface, defect contraction, bleeding attenuation, MDA- and NO-levels in cecum tissue at 15 min, and severity of cecum lesions and adhesions at 1 and 7 d.Post-injury, during/after a saline bath, the number of vessels was significantly reduced, the defect was slightly narrowed, bleeding was significant and MDA-levels increased and NO-levels decreased. BPC 157 bath: the vessel presentation was markedly increased, the defect was noticeably narrowed, the bleeding time was shortened and MDA- and NO-levels remained normal. L-NAME: reduced vessel presentation but not more than the control, did not change defect and shortened bleeding. L-arginine: exhibited less vessel reduction, did not change the defect and prolonged bleeding. In combination, mutual counteraction occurred (L-NAME + L-arginine) or the presentation was similar to that of BPC 157 rats (BPC 157 + L-NAME; BPC 157 + L-arginine; BPC 157 + L-NAME + L-arginine), except the defect did not change. Thereby at day 1 and 7, saline, L-NAME, L-arginine and L-NAME + L-arginine failed (defect was still open and large adhesions present).The therapeutic effect was achieved with BPC 157 alone or in combination with L-NAME and L-arginine as it was able to consolidate the stimulating and inhibiting effects of the NO-system towards more effective healing recruiting vessels. |
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| ISSN: | 10079327 |
| DOI: | 10.3748/wjg.v24.i48.5462 |