Synergistic Effects of a Calcium Phosphate/Fibronectin Coating on the Adhesion of Periodontal Ligament Stem Cells onto Decellularized Dental Root Surfaces
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| Titel: | Synergistic Effects of a Calcium Phosphate/Fibronectin Coating on the Adhesion of Periodontal Ligament Stem Cells onto Decellularized Dental Root Surfaces |
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| Autoren: | Jae Suk Jung, Tae Wan Kim, Hyunsuk Kim, Chang-Sung Kim, So Yon Park, Jong Bin Lee, Jung Seok Lee |
| Weitere Verfasser: | College of Dentistry, Dept. of Periodontology, Jung-Seok Lee, Hyun-Suk Kim, So-Yon Park, Tae-Wan Kim, Jae-Suk Jung, Jong-Bin Lee, Chang-Sung Kim, Kim, Chang Sung, Kim, Tae-Wan, Lee, Jung Seok |
| Quelle: | Cell Transplantation, Vol 24 (2015) |
| Verlagsinformationen: | SAGE Publications, 2015. |
| Publikationsjahr: | 2015 |
| Schlagwörter: | Calcium Phosphates, Male, Periodontal Ligament, Cells, Tooth Root/diagnostic imaging, Resistance, Fibronectins/chemistry, Transplantation, Autologous, 03 medical and health sciences, Dogs, 0302 clinical medicine, Antigens, CD, Autophagy, Cell Adhesion, Chemotherapy, Animals, Antigens, Tooth Root, Hypoxia, Cells, Cultured, 2. Zero hunger, Transplantation, Cultured, Stem Cells/metabolism, Periodontal Ligament/cytology, Stem Cells, Tooth/pathology, CD/metabolism, Calcium Phosphates/chemistry, X-Ray Microtomography, Fibronectins, Stem Cells/cytology, Medicine, Tooth Root/metabolism, Lung cancer, Autologous, Tooth, Tooth Root/cytology, Stem Cell Transplantation |
| Beschreibung: | This study aimed to enhance the attachment of periodontal ligament stem cells (PDLSCs) onto the decellularized dental root surface using surface coating with fibronectin and/or calcium phosphate (CaP) and to evaluate the activity of PDLSCs attached to a coated dental root surface following tooth replantation. PDLSCs were isolated from five dogs, and the other dental roots were used as a scaffold for carrying PDLSCs and then assigned to one of four groups according to whether their surface was coated with CaP, fibronectin, CaP/fibronectin, or left uncoated (control). Fibronectin increased the adhesion of PDLSCs onto dental root surfaces compared to both the control and CaP-coated groups, and simultaneous surface coating with CaP and fibronectin significantly accelerated and increased PDLSC adhesion compared to the fibronectin-only group. On in vivo tooth replantation, functionally oriented periodontal new attachment was observed on the CaP/fibronectin-coated dental roots to which autologous PDLSCs had adhered, while in the control condition, dental root replantation was associated only with root resorption and ankylosis along the entire root length. CaP and fibronectin synergistically enhanced the attachment of PDLSCs onto dental root surfaces, and autologous PDLSCs could produce de novo periodontal new attachment in an experimental in vivo model. |
| Publikationsart: | Article |
| Sprache: | English |
| ISSN: | 1555-3892 0963-6897 |
| DOI: | 10.3727/096368914x684628 |
| Zugangs-URL: | https://pubmed.ncbi.nlm.nih.gov/25290076 https://doaj.org/article/e5928f675b90482e8fea4d22cfc04d49 https://pubmed.ncbi.nlm.nih.gov/25290076/ https://journals.sagepub.com/doi/full/10.3727/096368914X684628 http://journals.sagepub.com/doi/10.3727/096368914X684628 https://yonsei.pure.elsevier.com/en/publications/synergistic-effects-of-a-calcium-phosphatefibronectin-coating-on- https://ir.ymlib.yonsei.ac.kr/handle/22282913/156756 https://journals.sagepub.com/doi/pdf/10.3727/096368914X684628 |
| Rights: | SAGE TDM CC BY NC ND |
| Dokumentencode: | edsair.doi.dedup.....369edaa70d853fe1cee44cd6b466ff91 |
| Datenbank: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstract: | This study aimed to enhance the attachment of periodontal ligament stem cells (PDLSCs) onto the decellularized dental root surface using surface coating with fibronectin and/or calcium phosphate (CaP) and to evaluate the activity of PDLSCs attached to a coated dental root surface following tooth replantation. PDLSCs were isolated from five dogs, and the other dental roots were used as a scaffold for carrying PDLSCs and then assigned to one of four groups according to whether their surface was coated with CaP, fibronectin, CaP/fibronectin, or left uncoated (control). Fibronectin increased the adhesion of PDLSCs onto dental root surfaces compared to both the control and CaP-coated groups, and simultaneous surface coating with CaP and fibronectin significantly accelerated and increased PDLSC adhesion compared to the fibronectin-only group. On in vivo tooth replantation, functionally oriented periodontal new attachment was observed on the CaP/fibronectin-coated dental roots to which autologous PDLSCs had adhered, while in the control condition, dental root replantation was associated only with root resorption and ankylosis along the entire root length. CaP and fibronectin synergistically enhanced the attachment of PDLSCs onto dental root surfaces, and autologous PDLSCs could produce de novo periodontal new attachment in an experimental in vivo model. |
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| ISSN: | 15553892 09636897 |
| DOI: | 10.3727/096368914x684628 |