Hydrophilic Interaction Liquid Chromatography for the Analysis of Pharmaceutical Formulations
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| Title: | Hydrophilic Interaction Liquid Chromatography for the Analysis of Pharmaceutical Formulations |
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| Authors: | Aleksandra Radoičić, Sandra Šegan, Aleksandra Dramićanin, Dušanka Milojković-Opsenica |
| Source: | Current Analytical Chemistry |
| Publisher Information: | Bentham Science Publishers Ltd., 2024. |
| Publication Year: | 2024 |
| Subject Terms: | Hydrophilic interaction liquid chromatography, polar stationary phase, hydrophilic partitioning, polar compounds, hydrogen bonding, electrostatic interactions, 01 natural sciences, pharmaceutical application, 0104 chemical sciences, 0105 earth and related environmental sciences |
| Description: | Abstract: For a long time, Reversed-Phase Liquid Chromatography (RPLC) was the most dominant technique for the analysis of pharmaceutical compounds, but with poor efficiency in the separation of small polar molecules. From the efforts to solve the problem of insufficient retention of these molecules, during the last decades, a mode of liquid chromatography named Hydrophilic Interaction Liquid Chromatography (HILIC) has experienced vast expansion. It is based on the use of a highly hydrophilic stationary phase along with an aqueous mobile phase with high organic modifier content. In this review, the characteristics of stationary and mobile phases used in HILIC are described, and corresponding separation mechanisms are discussed. An overview of recently published papers dealing with the application of HILIC in analyzing pharmaceuticals in biological and non-biological samples is provided. Besides, the application of HILIC systems in the determination of the physicochemical properties of compounds is described. |
| Document Type: | Article |
| Language: | English |
| ISSN: | 1573-4110 |
| DOI: | 10.2174/0115734110290557240305045032 |
| Access URL: | https://www.eurekaselect.com/article/139046 https://cherry.chem.bg.ac.rs/handle/123456789/6831 http://cer.ihtm.bg.ac.rs/bitstream/id/30117/bitstream_30117.pdf https://cer.ihtm.bg.ac.rs/handle/123456789/7609 |
| Accession Number: | edsair.doi.dedup.....2aa7bfee934b00b52987cb15def69e55 |
| Database: | OpenAIRE |
Nájsť tento článok vo Web of Science | Abstract: | Abstract: For a long time, Reversed-Phase Liquid Chromatography (RPLC) was the most dominant technique for the analysis of pharmaceutical compounds, but with poor efficiency in the separation of small polar molecules. From the efforts to solve the problem of insufficient retention of these molecules, during the last decades, a mode of liquid chromatography named Hydrophilic Interaction Liquid Chromatography (HILIC) has experienced vast expansion. It is based on the use of a highly hydrophilic stationary phase along with an aqueous mobile phase with high organic modifier content. In this review, the characteristics of stationary and mobile phases used in HILIC are described, and corresponding separation mechanisms are discussed. An overview of recently published papers dealing with the application of HILIC in analyzing pharmaceuticals in biological and non-biological samples is provided. Besides, the application of HILIC systems in the determination of the physicochemical properties of compounds is described. |
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| ISSN: | 15734110 |
| DOI: | 10.2174/0115734110290557240305045032 |