Laundry detergents and detergent residue after rinsing directly disrupt tight junction barrier integrity in human bronchial epithelial cells
Uloženo v:
| Název: | Laundry detergents and detergent residue after rinsing directly disrupt tight junction barrier integrity in human bronchial epithelial cells |
|---|---|
| Autoři: | Swati Acharya, Patrick Westermann, Anita Dreher, Kari C. Nadeau, Ming Wang, Chengshuo Wang, Cezmi A. Akdis, Ge Tan, Andrzej Eljaszewicz, Luo Zhang, Liying Yan, Paulina Wawrzyniak, Yifan Meng, Mübeccel Akdis, Can Altunbulakli |
| Přispěvatelé: | University of Zurich, Akdis, Cezmi A |
| Zdroj: | Journal of Allergy and Clinical Immunology. 143:1892-1903 |
| Informace o vydavateli: | Elsevier BV, 2019. |
| Rok vydání: | 2019 |
| Témata: | 0301 basic medicine, Alveolar epithelial cells - metabolism, Cells, Electric impedance, Immunology, Detergents, 610 Medicine & health, 10071 Functional Genomics Center Zurich, Apoptosis, Bronchi, Tight Junctions, 03 medical and health sciences, 10183 Swiss Institute of Allergy and Asthma Research, Electric Impedance, Immunology and Allergy, Alarmins, Humans, cultured, Cells, Cultured, Gene expression regulation, 2403 Immunology, 0303 health sciences, L-Lactate Dehydrogenase, Bronchi - pathology, L-lactate dehydrogenase - metabolism, High-Throughput Nucleotide Sequencing, Alarmins - metabolism, Lipid Metabolism, Tight junctions - metabolism, Asthma, 3. Good health, Lipid metabolism, Alarmins - genetics, Gene Expression Regulation, Alveolar Epithelial Cells, Tight junctions - pathology, 2723 Immunology and Allergy, Detergents - metabolism, Asthma - metabolism, Alveolar epithelial cells - pathology, High-throughput nucleotide sequencing |
| Popis: | Defects in the epithelial barrier have recently been associated with asthma and other allergies. The influence of laundry detergents on human bronchial epithelial cells (HBECs) and their barrier function remain unknown.We investigated the effects of laundry detergents on cytotoxicity, barrier function, the transcriptome, and the epigenome in HBECs.Air-liquid interface cultures of primary HBECs from healthy control subjects, patients with asthma, and patients with chronic obstructive pulmonary disease were exposed to laundry detergents and detergent residue after rinsing. Cytotoxicity and epithelial barrier function were evaluated. RNA sequencing, Assay for Transposase Accessible Chromatin with high-throughput sequencing, and DNA methylation arrays were used for checking the transcriptome and epigenome.Laundry detergents and rinse residue showed dose-dependent toxic effects on HBECs, with irregular cell shape and leakage of lactate dehydrogenase after 24 hours of exposure. A disrupted epithelial barrier function was found with decreased transepithelial electrical resistance, increased paracellular flux, and stratified tight junction (TJ) immunostaining in HBECs exposed to laundry detergent at 1:25,000 dilutions or rinse residue at further 1:10 dilutions. RNA sequencing analysis showed that lipid metabolism, apoptosis progress, and epithelially derived alarmin-related gene expression were upregulated, whereas cell adhesion-related gene expression was downregulated by laundry detergent at 1:50,000 dilutions after 24 hours of exposure without substantially affecting chromatin accessibility and DNA methylation.Our data demonstrate that laundry detergents, even at a very high dilution, and rinse residue show significant cell-toxic and directly disruptive effects on the TJ barrier integrity of HBECs without affecting the epigenome and TJ gene expression. |
| Druh dokumentu: | Article |
| Jazyk: | English |
| ISSN: | 0091-6749 |
| DOI: | 10.1016/j.jaci.2018.11.016 |
| DOI: | 10.5167/uzh-160480 |
| Přístupová URL adresa: | http://www.jacionline.org/article/S0091674918316658/pdf https://pubmed.ncbi.nlm.nih.gov/30500342 https://www.zora.uzh.ch/id/eprint/160480/ https://www.sciencedirect.com/science/article/abs/pii/S0091674918316658 https://www.jacionline.org/article/S0091-6749(18)31665-8/fulltext https://www.ncbi.nlm.nih.gov/pubmed/30500342 https://europepmc.org/article/MED/30500342 https://pubmed.ncbi.nlm.nih.gov/30500342/ https://www.sciencedirect.com/science/article/pii/S0091674918316658?via=ihub https://doi.org/10.1016/j.jaci.2018.11.016 |
| Rights: | Elsevier TDM |
| Přístupové číslo: | edsair.doi.dedup.....1a8dd64ec4d4253c12b3d3c87910eeb0 |
| Databáze: | OpenAIRE |
Full Text Finder 
Nájsť tento článok vo Web of Science | Abstrakt: | Defects in the epithelial barrier have recently been associated with asthma and other allergies. The influence of laundry detergents on human bronchial epithelial cells (HBECs) and their barrier function remain unknown.We investigated the effects of laundry detergents on cytotoxicity, barrier function, the transcriptome, and the epigenome in HBECs.Air-liquid interface cultures of primary HBECs from healthy control subjects, patients with asthma, and patients with chronic obstructive pulmonary disease were exposed to laundry detergents and detergent residue after rinsing. Cytotoxicity and epithelial barrier function were evaluated. RNA sequencing, Assay for Transposase Accessible Chromatin with high-throughput sequencing, and DNA methylation arrays were used for checking the transcriptome and epigenome.Laundry detergents and rinse residue showed dose-dependent toxic effects on HBECs, with irregular cell shape and leakage of lactate dehydrogenase after 24 hours of exposure. A disrupted epithelial barrier function was found with decreased transepithelial electrical resistance, increased paracellular flux, and stratified tight junction (TJ) immunostaining in HBECs exposed to laundry detergent at 1:25,000 dilutions or rinse residue at further 1:10 dilutions. RNA sequencing analysis showed that lipid metabolism, apoptosis progress, and epithelially derived alarmin-related gene expression were upregulated, whereas cell adhesion-related gene expression was downregulated by laundry detergent at 1:50,000 dilutions after 24 hours of exposure without substantially affecting chromatin accessibility and DNA methylation.Our data demonstrate that laundry detergents, even at a very high dilution, and rinse residue show significant cell-toxic and directly disruptive effects on the TJ barrier integrity of HBECs without affecting the epigenome and TJ gene expression. |
|---|---|
| ISSN: | 00916749 |
| DOI: | 10.1016/j.jaci.2018.11.016 |