Assessment of focal renal ischemia–reperfusion injury in a porcine model using hyperpolarized [1‐13C]pyruvate MRI
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| Názov: | Assessment of focal renal ischemia–reperfusion injury in a porcine model using hyperpolarized [1‐13C]pyruvate MRI |
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| Autori: | Uffe Kjærgaard, Nikolaj Bøgh, Esben Søvsø Szocska Hansen, Rasmus Stilling Tougaard, Lotte Bonde Bertelsen, Rolf F. Schulte, Christoffer Laustsen |
| Zdroj: | Kjaergaard, U, Bøgh, N, Hansen, E S S, Tougaard, R S, Bertelsen, L B, Schulte, R F & Laustsen, C 2023, 'Assessment of focal renal ischemia-reperfusion injury in a porcine model using hyperpolarized [1-13 C]pyruvate MRI', Magnetic Resonance in Medicine, vol. 90, no. 2, pp. 655-663. https://doi.org/10.1002/mrm.29649 |
| Informácie o vydavateľovi: | Wiley, 2023. |
| Rok vydania: | 2023 |
| Predmety: | Reperfusion Injury/diagnostic imaging, Alanine, Swine, ischemia, Kidney, Magnetic Resonance Imaging, Magnetic Resonance Imaging/methods, 3. Good health, Pyruvic Acid/metabolism, Bicarbonates, acute kidney injury, Reperfusion Injury, Pyruvic Acid, Animals, Kidney/diagnostic imaging, Bicarbonates/metabolism, Lactic Acid, Lactic Acid/metabolism, hyperpolarization, MRI, Alanine/metabolism |
| Popis: | PurposeIschemic injury in the kidney is a common pathophysiological event associated with both acute kidney injury and chronic kidney disease; however, regional ischemia–reperfusion as seen in thromboembolic renal disease is often undetectable and thus subclinical. Here, we assessed the metabolic alterations following subclinical focal ischemia–reperfusion injury with hyperpolarized [1‐13C]pyruvate MRI in a porcine model.MethodsFive pigs were subjected to 60 min of focal kidney ischemia. After 90 min of reperfusion, a multiparametric proton MRI protocol was performed on a clinical 3T scanner system. Metabolism was evaluated using 13C MRI following infusion of hyperpolarized [1‐13C]pyruvate. Ratios of pyruvate to its detectable metabolites (lactate, bicarbonate, and alanine) were used to quantify metabolism.ResultsThe focal ischemia–reperfusion injury resulted in injured areas with a mean size of 0.971 cm3 (±1.019). Compared with the contralateral kidney, the injured areas demonstrated restricted diffusion (1269 ± 83.59 × 10−6 mm2/s vs. 1530 ± 52.73 × 10−6 mm2/s; p = 0.006) and decreased perfusion (158.8 ± 29.4 mL/100 mL/min vs. 274 ± 63.1 mL/100 mL/min; p = 0.014). In the metabolic assessment, the injured areas displayed increased lactate/pyruvate ratios compared with the entire ipsilateral and the contralateral kidney (0.35 ± 0.13 vs. 0.27 ± 0.1 vs. 0.25 ± 0.1; p = 0.0086). Alanine/pyruvate ratio was unaltered, and we were unable to quantify bicarbonate due to low signal.ConclusionMRI with hyperpolarized [1‐13C]pyruvate in a clinical setup is capable of detecting the acute, subtle, focal metabolic changes following ischemia. This may prove to be a valuable future addition to the renal MRI suite. |
| Druh dokumentu: | Article |
| Popis súboru: | application/pdf |
| Jazyk: | English |
| ISSN: | 1522-2594 0740-3194 |
| DOI: | 10.1002/mrm.29649 |
| Prístupová URL adresa: | https://pubmed.ncbi.nlm.nih.gov/36971340 https://pure.au.dk/portal/en/publications/cbc4e3ad-dea4-4708-bf67-0f583065fb30 |
| Rights: | CC BY |
| Prístupové číslo: | edsair.doi.dedup.....03a7571d173f8883b66f50e8a86b70c5 |
| Databáza: | OpenAIRE |
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Nájsť tento článok vo Web of Science | Abstrakt: | PurposeIschemic injury in the kidney is a common pathophysiological event associated with both acute kidney injury and chronic kidney disease; however, regional ischemia–reperfusion as seen in thromboembolic renal disease is often undetectable and thus subclinical. Here, we assessed the metabolic alterations following subclinical focal ischemia–reperfusion injury with hyperpolarized [1‐13C]pyruvate MRI in a porcine model.MethodsFive pigs were subjected to 60 min of focal kidney ischemia. After 90 min of reperfusion, a multiparametric proton MRI protocol was performed on a clinical 3T scanner system. Metabolism was evaluated using 13C MRI following infusion of hyperpolarized [1‐13C]pyruvate. Ratios of pyruvate to its detectable metabolites (lactate, bicarbonate, and alanine) were used to quantify metabolism.ResultsThe focal ischemia–reperfusion injury resulted in injured areas with a mean size of 0.971 cm3 (±1.019). Compared with the contralateral kidney, the injured areas demonstrated restricted diffusion (1269 ± 83.59 × 10−6 mm2/s vs. 1530 ± 52.73 × 10−6 mm2/s; p = 0.006) and decreased perfusion (158.8 ± 29.4 mL/100 mL/min vs. 274 ± 63.1 mL/100 mL/min; p = 0.014). In the metabolic assessment, the injured areas displayed increased lactate/pyruvate ratios compared with the entire ipsilateral and the contralateral kidney (0.35 ± 0.13 vs. 0.27 ± 0.1 vs. 0.25 ± 0.1; p = 0.0086). Alanine/pyruvate ratio was unaltered, and we were unable to quantify bicarbonate due to low signal.ConclusionMRI with hyperpolarized [1‐13C]pyruvate in a clinical setup is capable of detecting the acute, subtle, focal metabolic changes following ischemia. This may prove to be a valuable future addition to the renal MRI suite. |
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| ISSN: | 15222594 07403194 |
| DOI: | 10.1002/mrm.29649 |