Mucosal vaccination with long-form TSLP induces migratory cDC1-mediated adaptive immunity against SARS-CoV-2 infection
Uloženo v:
| Název: | Mucosal vaccination with long-form TSLP induces migratory cDC1-mediated adaptive immunity against SARS-CoV-2 infection |
|---|---|
| Autoři: | Jing Hu, Housheng Zheng, Wei Ran, Xuefei Wang, Chenghui Liao, Jian Zhou, Liang Ye |
| Zdroj: | Journal of Virology. 99 |
| Informace o vydavateli: | American Society for Microbiology, 2025. |
| Rok vydání: | 2025 |
| Popis: | A combination of vaccination strategies will potentially be required for effective control of the virus pandemic. We report that mice intranasally immunized with commercial severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) subunit vaccines enriched with the human thymic stromal lymphopoietin (TSLP) variant, long-form TSLP (lfTSLP), but not the short-isoform TSLP (sfTSLP), induced robust antigen-specific systemic and mucosal antibody production. The adjuvant-enhancing activity of lfTSLP in mice requires functional TSLP receptor signals in migratory type 1 conventional dendritic cells (cDC1s). Furthermore, lfTSLP acts on migratory cDC1s to enhance T follicular helper (Tfh) cell and germinal center (GC) B cell responses. Intranasal vaccination with lfTSLP elicits long-lasting immunogenicity and protection against the challenge of wild-type SARS-CoV-2 and the B.1.617.2 variant in mice. Our study provides insights into the adjuvant role of lfTSLP, which is critical in enhancing migratory cDC1-mediated GC responses to improve vaccine efficacy. IMPORTANCE Adjuvants are indispensable components of subunit vaccines, and the development of adjuvants capable of inducing powerful systemic and mucosal immune responses is critical for enhancing the efficacy of viral vaccines. This study reveals that the human long-form thymic stromal lymphopoietin (lfTSLP) induces antigen-specific systemic IgG and mucosal IgA antibody production with sustained immunogenicity. Mechanistically, lfTSLP enhances germinal center reactions by preferentially activating migratory type 1 conventional dendritic cells (cDC1s). These findings uncover a previously unrecognized mechanism underlying the adjuvant activity of lfTSLP, which enhances vaccine-induced adaptive immunity and confers protection against SARS-CoV-2 infection. These findings indicate that the application of lfTSLP as an adjuvant should be encouraged in the rational design and development of viral vaccines. |
| Druh dokumentu: | Article |
| Jazyk: | English |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.01231-25 |
| Rights: | CC BY |
| Přístupové číslo: | edsair.doi...........d9c82bb7f08c80701906a2c0d6984696 |
| Databáze: | OpenAIRE |
Nájsť tento článok vo Web of Science | FullText | Text: Availability: 0 CustomLinks: – Url: https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=EBSCO&SrcAuth=EBSCO&DestApp=WOS&ServiceName=TransferToWoS&DestLinkType=GeneralSearchSummary&Func=Links&author=Hu%20J Name: ISI Category: fullText Text: Nájsť tento článok vo Web of Science Icon: https://imagesrvr.epnet.com/ls/20docs.gif MouseOverText: Nájsť tento článok vo Web of Science |
|---|---|
| Header | DbId: edsair DbLabel: OpenAIRE An: edsair.doi...........d9c82bb7f08c80701906a2c0d6984696 RelevancyScore: 1002 AccessLevel: 3 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 1002.22900390625 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Mucosal vaccination with long-form TSLP induces migratory cDC1-mediated adaptive immunity against SARS-CoV-2 infection – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Jing+Hu%22">Jing Hu</searchLink><br /><searchLink fieldCode="AR" term="%22Housheng+Zheng%22">Housheng Zheng</searchLink><br /><searchLink fieldCode="AR" term="%22Wei+Ran%22">Wei Ran</searchLink><br /><searchLink fieldCode="AR" term="%22Xuefei+Wang%22">Xuefei Wang</searchLink><br /><searchLink fieldCode="AR" term="%22Chenghui+Liao%22">Chenghui Liao</searchLink><br /><searchLink fieldCode="AR" term="%22Jian+Zhou%22">Jian Zhou</searchLink><br /><searchLink fieldCode="AR" term="%22Liang+Ye%22">Liang Ye</searchLink> – Name: TitleSource Label: Source Group: Src Data: <i>Journal of Virology</i>. 99 – Name: Publisher Label: Publisher Information Group: PubInfo Data: American Society for Microbiology, 2025. – Name: DatePubCY Label: Publication Year Group: Date Data: 2025 – Name: Abstract Label: Description Group: Ab Data: A combination of vaccination strategies will potentially be required for effective control of the virus pandemic. We report that mice intranasally immunized with commercial severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) subunit vaccines enriched with the human thymic stromal lymphopoietin (TSLP) variant, long-form TSLP (lfTSLP), but not the short-isoform TSLP (sfTSLP), induced robust antigen-specific systemic and mucosal antibody production. The adjuvant-enhancing activity of lfTSLP in mice requires functional TSLP receptor signals in migratory type 1 conventional dendritic cells (cDC1s). Furthermore, lfTSLP acts on migratory cDC1s to enhance T follicular helper (Tfh) cell and germinal center (GC) B cell responses. Intranasal vaccination with lfTSLP elicits long-lasting immunogenicity and protection against the challenge of wild-type SARS-CoV-2 and the B.1.617.2 variant in mice. Our study provides insights into the adjuvant role of lfTSLP, which is critical in enhancing migratory cDC1-mediated GC responses to improve vaccine efficacy. IMPORTANCE Adjuvants are indispensable components of subunit vaccines, and the development of adjuvants capable of inducing powerful systemic and mucosal immune responses is critical for enhancing the efficacy of viral vaccines. This study reveals that the human long-form thymic stromal lymphopoietin (lfTSLP) induces antigen-specific systemic IgG and mucosal IgA antibody production with sustained immunogenicity. Mechanistically, lfTSLP enhances germinal center reactions by preferentially activating migratory type 1 conventional dendritic cells (cDC1s). These findings uncover a previously unrecognized mechanism underlying the adjuvant activity of lfTSLP, which enhances vaccine-induced adaptive immunity and confers protection against SARS-CoV-2 infection. These findings indicate that the application of lfTSLP as an adjuvant should be encouraged in the rational design and development of viral vaccines. – Name: TypeDocument Label: Document Type Group: TypDoc Data: Article – Name: Language Label: Language Group: Lang Data: English – Name: ISSN Label: ISSN Group: ISSN Data: 1098-5514<br />0022-538X – Name: DOI Label: DOI Group: ID Data: 10.1128/jvi.01231-25 – Name: Copyright Label: Rights Group: Cpyrght Data: CC BY – Name: AN Label: Accession Number Group: ID Data: edsair.doi...........d9c82bb7f08c80701906a2c0d6984696 |
| PLink | https://erproxy.cvtisr.sk/sfx/access?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsair&AN=edsair.doi...........d9c82bb7f08c80701906a2c0d6984696 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1128/jvi.01231-25 Languages: – Text: English Titles: – TitleFull: Mucosal vaccination with long-form TSLP induces migratory cDC1-mediated adaptive immunity against SARS-CoV-2 infection Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Jing Hu – PersonEntity: Name: NameFull: Housheng Zheng – PersonEntity: Name: NameFull: Wei Ran – PersonEntity: Name: NameFull: Xuefei Wang – PersonEntity: Name: NameFull: Chenghui Liao – PersonEntity: Name: NameFull: Jian Zhou – PersonEntity: Name: NameFull: Liang Ye IsPartOfRelationships: – BibEntity: Dates: – D: 23 M: 09 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 10985514 – Type: issn-print Value: 0022538X – Type: issn-locals Value: edsair – Type: issn-locals Value: edsairFT Numbering: – Type: volume Value: 99 Titles: – TitleFull: Journal of Virology Type: main |
| ResultId | 1 |