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NANOG is repurposed after implantation to repress Sox2 and begin pluripotency extinction

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Titel: NANOG is repurposed after implantation to repress Sox2 and begin pluripotency extinction
Autoren: Frederick C K Wong, Man Zhang, Ella Thomson, Linus J Schumacher, Anestis Tsakiridis, James Ashmore, Tong Li, Guillaume Blin, Eleni Karagianni, Nicholas P Mullin, Ian Chambers, Valerie Wilson
Quelle: The EMBO Journal. 44:5337-5374
Verlagsinformationen: Springer Science and Business Media LLC, 2025.
Publikationsjahr: 2025
Beschreibung: Loss of pluripotency is an essential step in post-implantation development that facilitates the emergence of somatic cell identities essential for gastrulation. Before implantation, pluripotent cell identity is governed by a gene regulatory network that includes the key transcription factors SOX2 and NANOG. However, it is unclear how the pluripotency gene regulatory network is dissolved to enable lineage restriction. Here, we show that SOX2 is required for post-implantation pluripotent identity in the mouse, and cells that lose SOX2 expression in the posterior epiblast are no longer pluripotent. Using in vitro and in vivo analyses, we demonstrate anticorrelated expression of NANOG and SOX2 preceding gastrulation, culminating in an early disappearance of pluripotent identity from posterior NANOGhigh/SOX2low epiblast. Surprisingly, Sox2 expression is repressed by NANOG and embryos with post-implantation deletion of Nanog maintain posterior SOX2 expression. Our results demonstrate that the distinctive features of post-implantation pluripotency are underpinned by altered functionality of pluripotency transcription factors, ensuring correct spatio-temporal loss of embryonic pluripotency.
Publikationsart: Article
Sprache: English
ISSN: 1460-2075
DOI: 10.1038/s44318-025-00527-9
Rights: CC BY
Dokumentencode: edsair.doi...........6851c88dcd1de3f73356b1491d3d6e7c
Datenbank: OpenAIRE
Beschreibung
Abstract:Loss of pluripotency is an essential step in post-implantation development that facilitates the emergence of somatic cell identities essential for gastrulation. Before implantation, pluripotent cell identity is governed by a gene regulatory network that includes the key transcription factors SOX2 and NANOG. However, it is unclear how the pluripotency gene regulatory network is dissolved to enable lineage restriction. Here, we show that SOX2 is required for post-implantation pluripotent identity in the mouse, and cells that lose SOX2 expression in the posterior epiblast are no longer pluripotent. Using in vitro and in vivo analyses, we demonstrate anticorrelated expression of NANOG and SOX2 preceding gastrulation, culminating in an early disappearance of pluripotent identity from posterior NANOGhigh/SOX2low epiblast. Surprisingly, Sox2 expression is repressed by NANOG and embryos with post-implantation deletion of Nanog maintain posterior SOX2 expression. Our results demonstrate that the distinctive features of post-implantation pluripotency are underpinned by altered functionality of pluripotency transcription factors, ensuring correct spatio-temporal loss of embryonic pluripotency.
ISSN:14602075
DOI:10.1038/s44318-025-00527-9