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Influence of the Geographic differences to the Genetic Variation and Distribution of Vector Borne Mosquito in Filariasis Endemic Areas of Central Java Indonesia

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Název: Influence of the Geographic differences to the Genetic Variation and Distribution of Vector Borne Mosquito in Filariasis Endemic Areas of Central Java Indonesia
Autoři: upiek ngesti wibawaning astuti, Hidayat Soesilohadi, Wisnu Nurcahyo, Suwarno Hadisusanto, Budi Mulyaningsih, Din Syafruddin
Informace o vydavateli: Springer Science and Business Media LLC, 2023.
Rok vydání: 2023
Témata: 3. Good health
Popis: Vector borne mosquito diseases are still as a public health problem in many countries in the world, including in Indonesia. Filariasis is a disease that is transmitted through mosquito bites and the cases are increasing in some areas in Indonesia. The information regarding the vectorCulex quinquefasciatusspecies distribution and the genetic variation in Indonesia have not been reported. The objective of the study was to determine the influence of geographyc differences to the distribution and genetic variation ofCx. quinquefasciatusthat was collected from 4 filariasis endemic areas in Central Java Indonesia. Mosquitoes were collected by using human landing collection method at night period. The mosquito distribution was analyzed with Kruskal Wallis test. DNAs of each mosquito specimens were then isolated and amplified by using 5 RAPD markers. DNA cluster analysis was done with MVSP™ software. A total of 396 mosquito specimens were collected and the PCR analysis revealed 945 fraghment, consisted of 40 monomorphic and 905 polymorphic fragments. The genetic similarities ranged from 0–75% in all collection areas. In conclusion, the study discovered up to a high genetic variation ofCx. quinquefasciatusand the distribution was significantly influenced by geographic position.
Druh dokumentu: Article
DOI: 10.21203/rs.3.rs-3055886/v1
Rights: CC BY
Přístupové číslo: edsair.doi...........622923f9bda17d11a1bcb408f3ca3dfa
Databáze: OpenAIRE
Popis
Abstrakt:Vector borne mosquito diseases are still as a public health problem in many countries in the world, including in Indonesia. Filariasis is a disease that is transmitted through mosquito bites and the cases are increasing in some areas in Indonesia. The information regarding the vectorCulex quinquefasciatusspecies distribution and the genetic variation in Indonesia have not been reported. The objective of the study was to determine the influence of geographyc differences to the distribution and genetic variation ofCx. quinquefasciatusthat was collected from 4 filariasis endemic areas in Central Java Indonesia. Mosquitoes were collected by using human landing collection method at night period. The mosquito distribution was analyzed with Kruskal Wallis test. DNAs of each mosquito specimens were then isolated and amplified by using 5 RAPD markers. DNA cluster analysis was done with MVSP™ software. A total of 396 mosquito specimens were collected and the PCR analysis revealed 945 fraghment, consisted of 40 monomorphic and 905 polymorphic fragments. The genetic similarities ranged from 0–75% in all collection areas. In conclusion, the study discovered up to a high genetic variation ofCx. quinquefasciatusand the distribution was significantly influenced by geographic position.
DOI:10.21203/rs.3.rs-3055886/v1