Performance of Culture Using a Semi-Automatic Needle as a Novel Tool for Collecting Lymph Node Samples for the Diagnosis of Canine Visceral Leishmaniasis.
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| Title: | Performance of Culture Using a Semi-Automatic Needle as a Novel Tool for Collecting Lymph Node Samples for the Diagnosis of Canine Visceral Leishmaniasis. |
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| Authors: | Mendes Júnior, Artur Augusto Velho, Figueiredo, Fabiano Borges, Ferreira, Luiz Cláudio, Keidel, Lucas, Ornellas, Renato Orsini, Almeida, Adilson Benedito, Santos, Fernanda Nunes, Miranda, Luciana de Freitas Campos, Marcelino, Andreza Pain, Pereira, Sandro Antonio, Menezes, Rodrigo Caldas |
| Source: | Animals (2076-2615); Jan2025, Vol. 15 Issue 1, p107, 9p |
| Subject Terms: | VISCERAL leishmaniasis, NEEDLE biopsy, LYMPH nodes, AUTOPSY, LEISHMANIA |
| Abstract: | Simple Summary: Visceral leishmaniasis caused by the protozoan Leishmania (Leishmania) infantum is a disease transmitted between dogs and humans. The current lymph node sampling techniques are unable to provide sufficient amounts of samples for laboratory tests. We, therefore, evaluated semi-automatic needle puncture as a novel tool for collecting lymph node samples for the diagnosis of canine visceral leishmaniasis. This technique uses a thick needle to increase the amount of sample and was compared with samples collected from the same lymph node by aspiration using a fine needle and by necropsy. Lymph node samples from 30 dogs seropositive for L. infantum were processed for use in the following laboratory tests: culture, immunohistochemistry, and histopathology. Leishmania spp. was detected by immunohistochemistry in 70% of the samples, and by histopathology in 33%. Culture positivity was 77% for the samples obtained by necropsy (87% in the first week), 73% for the samples obtained by fine needle aspiration (82% in the first week), and 63% for the samples obtained using semi-automatic needle puncture (95% in the first week). The combination of semi-automatic needle puncture and culture is an alternative for the detection of Leishmania spp. in the lymph nodes of dogs because of its efficiency, practicality, and fast results. Zoonotic visceral leishmaniasis is caused by Leishmania (Leishmania) infantum and dogs are the main domestic reservoir. This study compared the performance of parasitological tests using semi-automatic needle puncture (SANP) for collecting popliteal lymph node samples with samples collected from the same lymph node by fine needle aspiration puncture (FNAP) and by necropsy for the diagnosis of canine visceral leishmaniasis (CVL). Popliteal lymph node samples were collected from 30 CVL-seropositive dogs from an endemic region in Brazil. After clinical examination and euthanasia, samples were collected from the same lymph node by SANP, FNAP, and necropsy. The reference tests were culture, immunohistochemistry, and histopathology. Positivity for Leishmania spp. was 70% for immunohistochemistry and 33.3% for histopathology. Culture positivity using the different sampling techniques was 77% for necropsy (87% in the first week), 73% for FNAP (82% in the first week), and 63% for SANP (95% in the first week). The combination of SANP and culture proved to be an alternative for the diagnosis of Leishmania spp. in the lymph node samples of dogs because of its high positivity rate and because it is more practical and faster and has a shorter time to positivity by culture when compared to FNAP and necropsy sampling. [ABSTRACT FROM AUTHOR] |
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| Database: | Biomedical Index |
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