Differential Effect of Acute and Chronic Exercise on Cardiac Angiogenesis Regulator: The Role of mRNA HIF‐1α and Its Negative Regulators of In Vivo Study.
Saved in:
| Title: | Differential Effect of Acute and Chronic Exercise on Cardiac Angiogenesis Regulator: The Role of mRNA HIF‐1α and Its Negative Regulators of In Vivo Study. |
|---|---|
| Authors: | Karisa, Putri, Sylviana, Nova, Goenawan, Hanna, Fitrianti, Hanny Primadini, Setiawan, Khatra, Harleen |
| Source: | Cardiology Research & Practice; 11/27/2025, Vol. 2025, p1-11, 11p |
| Subject Terms: | MYOCARDIUM physiology, EXERCISE physiology, VASCULAR endothelial growth factors, T-test (Statistics), RESEARCH funding, EXERCISE therapy, KRUSKAL-Wallis Test, IN vivo studies, REVERSE transcriptase polymerase chain reaction, MANN Whitney U Test, DESCRIPTIVE statistics, MESSENGER RNA, GENE expression, RATS, EXPERIMENTAL design, OXIDOREDUCTASES, ANIMAL experimentation, AEROBIC exercises, TREADMILLS, ONE-way analysis of variance, COMPARATIVE studies, DATA analysis software, NEOVASCULARIZATION, TIME |
| Abstract: | Introduction: Angiogenesis is a critical adaptation to regular physical exercise, primarily driven by hypoxia‐inducible factor‐1 alpha (HIF‐1α). However, prolonged exercise has been associated with the downregulation of HIF‐1α, potentially mediated by increased expression of its negative regulators, prolyl hydroxylase domain (PHD) and factor‐inhibiting HIF‐1 (FIH). Objectives: This study aimed to investigate the effects of short‐term (acute) and long‐term (chronic) moderate‐intensity exercise on HIF‐1α, PHD, and FIH mRNA expression in Wistar rat hearts. Methods: Twenty Wistar rats (age: 8 weeks, body weight: 200–250 g) were divided into four groups: acute control (AC) (15 days) (n = 5), acute exercise (AE) (15 days) (n = 5), chronic control (CC) (8 weeks) (n = 5), and chronic exercise (CE) (8 weeks) (n = 5). The exercise groups underwent moderate‐intensity treadmill exercise with 20 m/min for 30 min each day for 5 times a week. At the end of the experiment, rats were sacrificed 1 h (acute group) and 24 h (chronic group) after exercise using isoflurane anesthesia, followed by cervical dislocation. Left ventricular heart muscle samples were collected for mRNA expression analysis of HIF‐1α, PHD, and FIH using real‐time PCR. Results: Exercise significantly altered the expression of HIF‐1α, PHD, and FIH. HIF‐1α mRNA was significantly higher in the AE group versus AC (AC vs AE, p = 0.006) and in the CE group versus CC (CC vs CE, p = 0.004). PHD expression likewise increased with exercise (AE vs AC, p = 0.001; CE vs CC, p ≤ 0.001). In contrast, FIH showed no significant differences (acute p = 0.472; chronic p = 0.095). Exploratory one‐way analyses confirmed overall group effects for HIF‐1α (p ≤ 0.001) and PHD (p = 0.016), but not for FIH (p = 0.105). Conclusion: Chronic moderate‐intensity exercise upregulates the expression of HIF‐1α negative regulators (PHD and FIH) in the myocardium, suggesting a shift from acute hypoxia‐driven responses to oxygen‐dependent regulation. These findings offer insight into the molecular adaptations of cardiac tissue to prolonged exercise and their potential role in angiogenesis regulation. [ABSTRACT FROM AUTHOR] |
| Copyright of Cardiology Research & Practice is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Complementary Index |
Full Text 
Full Text Finder 
Nájsť tento článok vo Web of Science Be the first to leave a comment!