Zobrazit v EDS

Differential Effect of Acute and Chronic Exercise on Cardiac Angiogenesis Regulator: The Role of mRNA HIF‐1α and Its Negative Regulators of In Vivo Study.

Uloženo v:
Podrobná bibliografie
Název: Differential Effect of Acute and Chronic Exercise on Cardiac Angiogenesis Regulator: The Role of mRNA HIF‐1α and Its Negative Regulators of In Vivo Study.
Autoři: Karisa, Putri, Sylviana, Nova, Goenawan, Hanna, Fitrianti, Hanny Primadini, Setiawan, Khatra, Harleen
Zdroj: Cardiology Research & Practice; 11/27/2025, Vol. 2025, p1-11, 11p
Témata: MYOCARDIUM physiology, EXERCISE physiology, VASCULAR endothelial growth factors, T-test (Statistics), RESEARCH funding, EXERCISE therapy, KRUSKAL-Wallis Test, IN vivo studies, REVERSE transcriptase polymerase chain reaction, MANN Whitney U Test, DESCRIPTIVE statistics, MESSENGER RNA, GENE expression, RATS, EXPERIMENTAL design, OXIDOREDUCTASES, ANIMAL experimentation, AEROBIC exercises, TREADMILLS, ONE-way analysis of variance, COMPARATIVE studies, DATA analysis software, NEOVASCULARIZATION, TIME
Abstrakt: Introduction: Angiogenesis is a critical adaptation to regular physical exercise, primarily driven by hypoxia‐inducible factor‐1 alpha (HIF‐1α). However, prolonged exercise has been associated with the downregulation of HIF‐1α, potentially mediated by increased expression of its negative regulators, prolyl hydroxylase domain (PHD) and factor‐inhibiting HIF‐1 (FIH). Objectives: This study aimed to investigate the effects of short‐term (acute) and long‐term (chronic) moderate‐intensity exercise on HIF‐1α, PHD, and FIH mRNA expression in Wistar rat hearts. Methods: Twenty Wistar rats (age: 8 weeks, body weight: 200–250 g) were divided into four groups: acute control (AC) (15 days) (n = 5), acute exercise (AE) (15 days) (n = 5), chronic control (CC) (8 weeks) (n = 5), and chronic exercise (CE) (8 weeks) (n = 5). The exercise groups underwent moderate‐intensity treadmill exercise with 20 m/min for 30 min each day for 5 times a week. At the end of the experiment, rats were sacrificed 1 h (acute group) and 24 h (chronic group) after exercise using isoflurane anesthesia, followed by cervical dislocation. Left ventricular heart muscle samples were collected for mRNA expression analysis of HIF‐1α, PHD, and FIH using real‐time PCR. Results: Exercise significantly altered the expression of HIF‐1α, PHD, and FIH. HIF‐1α mRNA was significantly higher in the AE group versus AC (AC vs AE, p = 0.006) and in the CE group versus CC (CC vs CE, p = 0.004). PHD expression likewise increased with exercise (AE vs AC, p = 0.001; CE vs CC, p ≤ 0.001). In contrast, FIH showed no significant differences (acute p = 0.472; chronic p = 0.095). Exploratory one‐way analyses confirmed overall group effects for HIF‐1α (p ≤ 0.001) and PHD (p = 0.016), but not for FIH (p = 0.105). Conclusion: Chronic moderate‐intensity exercise upregulates the expression of HIF‐1α negative regulators (PHD and FIH) in the myocardium, suggesting a shift from acute hypoxia‐driven responses to oxygen‐dependent regulation. These findings offer insight into the molecular adaptations of cardiac tissue to prolonged exercise and their potential role in angiogenesis regulation. [ABSTRACT FROM AUTHOR]
Copyright of Cardiology Research & Practice is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Databáze: Complementary Index
FullText Text:
  Availability: 0
CustomLinks:
  – Url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&db=pmc&term=2090-8016[TA]+AND+1[PG]+AND+2025[PDAT]
    Name: FREE - PubMed Central (ISSN based link)
    Category: fullText
    Text: Full Text
    Icon: https://imageserver.ebscohost.com/NetImages/iconPdf.gif
    MouseOverText: Check this PubMed for the article full text.
  – Url: https://resolver.ebscohost.com/openurl?sid=EBSCO:edb&genre=article&issn=20908016&ISBN=&volume=2025&issue=&date=20251127&spage=1&pages=1-11&title=Cardiology Research & Practice&atitle=Differential%20Effect%20of%20Acute%20and%20Chronic%20Exercise%20on%20Cardiac%20Angiogenesis%20Regulator%3A%20The%20Role%20of%20mRNA%20HIF%E2%80%901%CE%B1%20and%20Its%20Negative%20Regulators%20of%20In%20Vivo%20Study.&aulast=Karisa%2C%20Putri&id=DOI:10.1155/crp/6348392
    Name: Full Text Finder
    Category: fullText
    Text: Full Text Finder
    Icon: https://imageserver.ebscohost.com/branding/images/FTF.gif
    MouseOverText: Full Text Finder
  – Url: https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=EBSCO&SrcAuth=EBSCO&DestApp=WOS&ServiceName=TransferToWoS&DestLinkType=GeneralSearchSummary&Func=Links&author=Karisa%20P
    Name: ISI
    Category: fullText
    Text: Nájsť tento článok vo Web of Science
    Icon: https://imagesrvr.epnet.com/ls/20docs.gif
    MouseOverText: Nájsť tento článok vo Web of Science
Header DbId: edb
DbLabel: Complementary Index
An: 189685426
RelevancyScore: 1082
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 1082.14916992188
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Differential Effect of Acute and Chronic Exercise on Cardiac Angiogenesis Regulator: The Role of mRNA HIF‐1α and Its Negative Regulators of In Vivo Study.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Karisa%2C+Putri%22">Karisa, Putri</searchLink><br /><searchLink fieldCode="AR" term="%22Sylviana%2C+Nova%22">Sylviana, Nova</searchLink><br /><searchLink fieldCode="AR" term="%22Goenawan%2C+Hanna%22">Goenawan, Hanna</searchLink><br /><searchLink fieldCode="AR" term="%22Fitrianti%2C+Hanny+Primadini%22">Fitrianti, Hanny Primadini</searchLink><br /><searchLink fieldCode="AR" term="%22Setiawan%22">Setiawan</searchLink><br /><searchLink fieldCode="AR" term="%22Khatra%2C+Harleen%22">Khatra, Harleen</searchLink>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: Cardiology Research & Practice; 11/27/2025, Vol. 2025, p1-11, 11p
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22MYOCARDIUM+physiology%22">MYOCARDIUM physiology</searchLink><br /><searchLink fieldCode="DE" term="%22EXERCISE+physiology%22">EXERCISE physiology</searchLink><br /><searchLink fieldCode="DE" term="%22VASCULAR+endothelial+growth+factors%22">VASCULAR endothelial growth factors</searchLink><br /><searchLink fieldCode="DE" term="%22T-test+%28Statistics%29%22">T-test (Statistics)</searchLink><br /><searchLink fieldCode="DE" term="%22RESEARCH+funding%22">RESEARCH funding</searchLink><br /><searchLink fieldCode="DE" term="%22EXERCISE+therapy%22">EXERCISE therapy</searchLink><br /><searchLink fieldCode="DE" term="%22KRUSKAL-Wallis+Test%22">KRUSKAL-Wallis Test</searchLink><br /><searchLink fieldCode="DE" term="%22IN+vivo+studies%22">IN vivo studies</searchLink><br /><searchLink fieldCode="DE" term="%22REVERSE+transcriptase+polymerase+chain+reaction%22">REVERSE transcriptase polymerase chain reaction</searchLink><br /><searchLink fieldCode="DE" term="%22MANN+Whitney+U+Test%22">MANN Whitney U Test</searchLink><br /><searchLink fieldCode="DE" term="%22DESCRIPTIVE+statistics%22">DESCRIPTIVE statistics</searchLink><br /><searchLink fieldCode="DE" term="%22MESSENGER+RNA%22">MESSENGER RNA</searchLink><br /><searchLink fieldCode="DE" term="%22GENE+expression%22">GENE expression</searchLink><br /><searchLink fieldCode="DE" term="%22RATS%22">RATS</searchLink><br /><searchLink fieldCode="DE" term="%22EXPERIMENTAL+design%22">EXPERIMENTAL design</searchLink><br /><searchLink fieldCode="DE" term="%22OXIDOREDUCTASES%22">OXIDOREDUCTASES</searchLink><br /><searchLink fieldCode="DE" term="%22ANIMAL+experimentation%22">ANIMAL experimentation</searchLink><br /><searchLink fieldCode="DE" term="%22AEROBIC+exercises%22">AEROBIC exercises</searchLink><br /><searchLink fieldCode="DE" term="%22TREADMILLS%22">TREADMILLS</searchLink><br /><searchLink fieldCode="DE" term="%22ONE-way+analysis+of+variance%22">ONE-way analysis of variance</searchLink><br /><searchLink fieldCode="DE" term="%22COMPARATIVE+studies%22">COMPARATIVE studies</searchLink><br /><searchLink fieldCode="DE" term="%22DATA+analysis+software%22">DATA analysis software</searchLink><br /><searchLink fieldCode="DE" term="%22NEOVASCULARIZATION%22">NEOVASCULARIZATION</searchLink><br /><searchLink fieldCode="DE" term="%22TIME%22">TIME</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Introduction: Angiogenesis is a critical adaptation to regular physical exercise, primarily driven by hypoxia‐inducible factor‐1 alpha (HIF‐1α). However, prolonged exercise has been associated with the downregulation of HIF‐1α, potentially mediated by increased expression of its negative regulators, prolyl hydroxylase domain (PHD) and factor‐inhibiting HIF‐1 (FIH). Objectives: This study aimed to investigate the effects of short‐term (acute) and long‐term (chronic) moderate‐intensity exercise on HIF‐1α, PHD, and FIH mRNA expression in Wistar rat hearts. Methods: Twenty Wistar rats (age: 8 weeks, body weight: 200–250 g) were divided into four groups: acute control (AC) (15 days) (n = 5), acute exercise (AE) (15 days) (n = 5), chronic control (CC) (8 weeks) (n = 5), and chronic exercise (CE) (8 weeks) (n = 5). The exercise groups underwent moderate‐intensity treadmill exercise with 20 m/min for 30 min each day for 5 times a week. At the end of the experiment, rats were sacrificed 1 h (acute group) and 24 h (chronic group) after exercise using isoflurane anesthesia, followed by cervical dislocation. Left ventricular heart muscle samples were collected for mRNA expression analysis of HIF‐1α, PHD, and FIH using real‐time PCR. Results: Exercise significantly altered the expression of HIF‐1α, PHD, and FIH. HIF‐1α mRNA was significantly higher in the AE group versus AC (AC vs AE, p = 0.006) and in the CE group versus CC (CC vs CE, p = 0.004). PHD expression likewise increased with exercise (AE vs AC, p = 0.001; CE vs CC, p ≤ 0.001). In contrast, FIH showed no significant differences (acute p = 0.472; chronic p = 0.095). Exploratory one‐way analyses confirmed overall group effects for HIF‐1α (p ≤ 0.001) and PHD (p = 0.016), but not for FIH (p = 0.105). Conclusion: Chronic moderate‐intensity exercise upregulates the expression of HIF‐1α negative regulators (PHD and FIH) in the myocardium, suggesting a shift from acute hypoxia‐driven responses to oxygen‐dependent regulation. These findings offer insight into the molecular adaptations of cardiac tissue to prolonged exercise and their potential role in angiogenesis regulation. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label:
  Group: Ab
  Data: <i>Copyright of Cardiology Research & Practice is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://erproxy.cvtisr.sk/sfx/access?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=189685426
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1155/crp/6348392
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 11
        StartPage: 1
    Subjects:
      – SubjectFull: MYOCARDIUM physiology
        Type: general
      – SubjectFull: EXERCISE physiology
        Type: general
      – SubjectFull: VASCULAR endothelial growth factors
        Type: general
      – SubjectFull: T-test (Statistics)
        Type: general
      – SubjectFull: RESEARCH funding
        Type: general
      – SubjectFull: EXERCISE therapy
        Type: general
      – SubjectFull: KRUSKAL-Wallis Test
        Type: general
      – SubjectFull: IN vivo studies
        Type: general
      – SubjectFull: REVERSE transcriptase polymerase chain reaction
        Type: general
      – SubjectFull: MANN Whitney U Test
        Type: general
      – SubjectFull: DESCRIPTIVE statistics
        Type: general
      – SubjectFull: MESSENGER RNA
        Type: general
      – SubjectFull: GENE expression
        Type: general
      – SubjectFull: RATS
        Type: general
      – SubjectFull: EXPERIMENTAL design
        Type: general
      – SubjectFull: OXIDOREDUCTASES
        Type: general
      – SubjectFull: ANIMAL experimentation
        Type: general
      – SubjectFull: AEROBIC exercises
        Type: general
      – SubjectFull: TREADMILLS
        Type: general
      – SubjectFull: ONE-way analysis of variance
        Type: general
      – SubjectFull: COMPARATIVE studies
        Type: general
      – SubjectFull: DATA analysis software
        Type: general
      – SubjectFull: NEOVASCULARIZATION
        Type: general
      – SubjectFull: TIME
        Type: general
    Titles:
      – TitleFull: Differential Effect of Acute and Chronic Exercise on Cardiac Angiogenesis Regulator: The Role of mRNA HIF‐1α and Its Negative Regulators of In Vivo Study.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Karisa, Putri
      – PersonEntity:
          Name:
            NameFull: Sylviana, Nova
      – PersonEntity:
          Name:
            NameFull: Goenawan, Hanna
      – PersonEntity:
          Name:
            NameFull: Fitrianti, Hanny Primadini
      – PersonEntity:
          Name:
            NameFull: Setiawan
      – PersonEntity:
          Name:
            NameFull: Khatra, Harleen
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 27
              M: 11
              Text: 11/27/2025
              Type: published
              Y: 2025
          Identifiers:
            – Type: issn-print
              Value: 20908016
          Numbering:
            – Type: volume
              Value: 2025
          Titles:
            – TitleFull: Cardiology Research & Practice
              Type: main
ResultId 1