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Boosting Probiotic Biomass of Lactobacillus acidophilus CCFM137 Through pH-Stat Morphological Control and Medium Optimization.

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Název: Boosting Probiotic Biomass of Lactobacillus acidophilus CCFM137 Through pH-Stat Morphological Control and Medium Optimization.
Autoři: Yan, Shao-Quan, Shi, Yang-Yang, Yang, Rui, Li, Rui, Hang, Feng, Zhang, Hao
Zdroj: Fermentation (Basel); Oct2025, Vol. 11 Issue 10, p564, 16p
Témata: LACTOBACILLUS acidophilus, FERMENTATION, CELL proliferation, CULTURE media (Biology), BIOMASS production, PROBIOTICS, ACID-base equilibrium, INDUSTRIAL applications
Abstrakt: The fermentation performance of Lactobacillus acidophilus is constrained by factors such as low cell density and fastidious nutritional and environmental requirements, which greatly limit its industrial-scale applications. This study aimed to develop an efficient fermentation condition for L. acidophilus CCFM137 through systematic optimization of both culture medium and environmental parameters, thereby enabling high-yield industrial-scale production of this strain. An optimized medium was developed, consisting of glucose (30 g/L), YEP FM503 (35 g/L), sodium acetate (5 g/L), ammonium citrate (2 g/L), K2HPO4 (2 g/L), MgSO4·7H2O (0.1 g/L), MnSO4·H2O (0.05 g/L), L-cysteine hydrochloride (0.5 g/L), and Tween 80 (1 mL/L), to achieve a viable cell count of 1.95 × 109 CFU/mL, representing a 9.42-fold increase over that of standard MRS broth. Subsequent pH-stat fermentation trials in a 100 L fermenter using the optimized medium revealed morphological and growth characteristics of the strain in variable pH-stat environments. Optimal performance was observed under pH-stat 4.5 rather than the more commonly used 5.7, achieving maximum viable cell counts of 3.37 × 109 CFU/mL, accompanied by a transformation of cell morphology toward shorter rod-shaped structures, as well as an increase in substrate utilization rate, cell recovery rate and lyophilization survival rate. The fermentation performance and cellular morphology of L. acidophilus CCFM137 were enhanced by both nutrient composition and pH environment. These results showed that this strategy has potential for application in high cell density fermentation of L. acidophilus CCFM137. [ABSTRACT FROM AUTHOR]
Copyright of Fermentation (Basel) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Databáze: Complementary Index
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  Data: Boosting Probiotic Biomass of Lactobacillus acidophilus CCFM137 Through pH-Stat Morphological Control and Medium Optimization.
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  Data: <searchLink fieldCode="AR" term="%22Yan%2C+Shao-Quan%22">Yan, Shao-Quan</searchLink><br /><searchLink fieldCode="AR" term="%22Shi%2C+Yang-Yang%22">Shi, Yang-Yang</searchLink><br /><searchLink fieldCode="AR" term="%22Yang%2C+Rui%22">Yang, Rui</searchLink><br /><searchLink fieldCode="AR" term="%22Li%2C+Rui%22">Li, Rui</searchLink><br /><searchLink fieldCode="AR" term="%22Hang%2C+Feng%22">Hang, Feng</searchLink><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Hao%22">Zhang, Hao</searchLink>
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  Data: Fermentation (Basel); Oct2025, Vol. 11 Issue 10, p564, 16p
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  Data: <searchLink fieldCode="DE" term="%22LACTOBACILLUS+acidophilus%22">LACTOBACILLUS acidophilus</searchLink><br /><searchLink fieldCode="DE" term="%22FERMENTATION%22">FERMENTATION</searchLink><br /><searchLink fieldCode="DE" term="%22CELL+proliferation%22">CELL proliferation</searchLink><br /><searchLink fieldCode="DE" term="%22CULTURE+media+%28Biology%29%22">CULTURE media (Biology)</searchLink><br /><searchLink fieldCode="DE" term="%22BIOMASS+production%22">BIOMASS production</searchLink><br /><searchLink fieldCode="DE" term="%22PROBIOTICS%22">PROBIOTICS</searchLink><br /><searchLink fieldCode="DE" term="%22ACID-base+equilibrium%22">ACID-base equilibrium</searchLink><br /><searchLink fieldCode="DE" term="%22INDUSTRIAL+applications%22">INDUSTRIAL applications</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The fermentation performance of Lactobacillus acidophilus is constrained by factors such as low cell density and fastidious nutritional and environmental requirements, which greatly limit its industrial-scale applications. This study aimed to develop an efficient fermentation condition for L. acidophilus CCFM137 through systematic optimization of both culture medium and environmental parameters, thereby enabling high-yield industrial-scale production of this strain. An optimized medium was developed, consisting of glucose (30 g/L), YEP FM503 (35 g/L), sodium acetate (5 g/L), ammonium citrate (2 g/L), K<subscript>2</subscript>HPO<subscript>4</subscript> (2 g/L), MgSO<subscript>4</subscript>·7H<subscript>2</subscript>O (0.1 g/L), MnSO<subscript>4</subscript>·H<subscript>2</subscript>O (0.05 g/L), L-cysteine hydrochloride (0.5 g/L), and Tween 80 (1 mL/L), to achieve a viable cell count of 1.95 × 10<superscript>9</superscript> CFU/mL, representing a 9.42-fold increase over that of standard MRS broth. Subsequent pH-stat fermentation trials in a 100 L fermenter using the optimized medium revealed morphological and growth characteristics of the strain in variable pH-stat environments. Optimal performance was observed under pH-stat 4.5 rather than the more commonly used 5.7, achieving maximum viable cell counts of 3.37 × 10<superscript>9</superscript> CFU/mL, accompanied by a transformation of cell morphology toward shorter rod-shaped structures, as well as an increase in substrate utilization rate, cell recovery rate and lyophilization survival rate. The fermentation performance and cellular morphology of L. acidophilus CCFM137 were enhanced by both nutrient composition and pH environment. These results showed that this strategy has potential for application in high cell density fermentation of L. acidophilus CCFM137. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label:
  Group: Ab
  Data: <i>Copyright of Fermentation (Basel) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.3390/fermentation11100564
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        Text: English
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      – SubjectFull: FERMENTATION
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      – SubjectFull: ACID-base equilibrium
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      – SubjectFull: INDUSTRIAL applications
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              Text: Oct2025
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