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Evaluation of Spleen Swabs for Sensitive and High-Throughput Detection of Classical Swine Fever Virus.

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Titel: Evaluation of Spleen Swabs for Sensitive and High-Throughput Detection of Classical Swine Fever Virus.
Autoren: Hochman, Orie, Goonewardene, Kalhari, Chung, Chungwon J., Ambagala, Aruna
Quelle: Pathogens; Aug2025, Vol. 14 Issue 8, p767, 14p
Schlagwörter: CLASSICAL swine fever, CLASSICAL swine fever virus, NUCLEIC acids, INSPECTION & review, DETECTION algorithms, SPLEEN, STATISTICAL reliability, REVERSE transcriptase polymerase chain reaction
Abstract: Despite intensive eradication efforts, classical swine fever (CSF) remains endemic across South America, Europe, Asia, and the Caribbean, highlighting the need for more effective surveillance and detection methods. Reverse-transcription real-time polymerase chain reaction (RRT-PCR) is the fastest, and most sensitive assay for detecting CSF virus (CSFV) genomic material. Previously, we demonstrated that spleen swabs outperformed spleen homogenates for the detection of ASFV genomic material by RRT-PCR. In this study, we compared CSFV genome detection in paired spleen homogenates and spleen swabs generated using 49 frozen and 33 fresh spleen samples collected from experimentally inoculated pigs with acute infection. The results show that the CSFV genome detection in spleen swabs is comparable to that in spleen homogenates. The study also demonstrated that the CSFV genomic material can be detected in spleen swabs during early CSFV infections, and the viruses can be successfully isolated from the swabs. The use of spleen swabs instead of spleen tissue homogenates for CSF detection will reduce labor, decrease costs associated with reporting, and increase the diagnostic throughput. [ABSTRACT FROM AUTHOR]
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  Label: Title
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  Data: Evaluation of Spleen Swabs for Sensitive and High-Throughput Detection of Classical Swine Fever Virus.
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  Data: <searchLink fieldCode="AR" term="%22Hochman%2C+Orie%22">Hochman, Orie</searchLink><br /><searchLink fieldCode="AR" term="%22Goonewardene%2C+Kalhari%22">Goonewardene, Kalhari</searchLink><br /><searchLink fieldCode="AR" term="%22Chung%2C+Chungwon+J%2E%22">Chung, Chungwon J.</searchLink><br /><searchLink fieldCode="AR" term="%22Ambagala%2C+Aruna%22">Ambagala, Aruna</searchLink>
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  Data: Pathogens; Aug2025, Vol. 14 Issue 8, p767, 14p
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  Data: <searchLink fieldCode="DE" term="%22CLASSICAL+swine+fever%22">CLASSICAL swine fever</searchLink><br /><searchLink fieldCode="DE" term="%22CLASSICAL+swine+fever+virus%22">CLASSICAL swine fever virus</searchLink><br /><searchLink fieldCode="DE" term="%22NUCLEIC+acids%22">NUCLEIC acids</searchLink><br /><searchLink fieldCode="DE" term="%22INSPECTION+%26+review%22">INSPECTION & review</searchLink><br /><searchLink fieldCode="DE" term="%22DETECTION+algorithms%22">DETECTION algorithms</searchLink><br /><searchLink fieldCode="DE" term="%22SPLEEN%22">SPLEEN</searchLink><br /><searchLink fieldCode="DE" term="%22STATISTICAL+reliability%22">STATISTICAL reliability</searchLink><br /><searchLink fieldCode="DE" term="%22REVERSE+transcriptase+polymerase+chain+reaction%22">REVERSE transcriptase polymerase chain reaction</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Despite intensive eradication efforts, classical swine fever (CSF) remains endemic across South America, Europe, Asia, and the Caribbean, highlighting the need for more effective surveillance and detection methods. Reverse-transcription real-time polymerase chain reaction (RRT-PCR) is the fastest, and most sensitive assay for detecting CSF virus (CSFV) genomic material. Previously, we demonstrated that spleen swabs outperformed spleen homogenates for the detection of ASFV genomic material by RRT-PCR. In this study, we compared CSFV genome detection in paired spleen homogenates and spleen swabs generated using 49 frozen and 33 fresh spleen samples collected from experimentally inoculated pigs with acute infection. The results show that the CSFV genome detection in spleen swabs is comparable to that in spleen homogenates. The study also demonstrated that the CSFV genomic material can be detected in spleen swabs during early CSFV infections, and the viruses can be successfully isolated from the swabs. The use of spleen swabs instead of spleen tissue homogenates for CSF detection will reduce labor, decrease costs associated with reporting, and increase the diagnostic throughput. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label:
  Group: Ab
  Data: <i>Copyright of Pathogens is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.3390/pathogens14080767
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      – Code: eng
        Text: English
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        PageCount: 14
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      – SubjectFull: CLASSICAL swine fever
        Type: general
      – SubjectFull: CLASSICAL swine fever virus
        Type: general
      – SubjectFull: NUCLEIC acids
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      – SubjectFull: DETECTION algorithms
        Type: general
      – SubjectFull: SPLEEN
        Type: general
      – SubjectFull: STATISTICAL reliability
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      – SubjectFull: REVERSE transcriptase polymerase chain reaction
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      – TitleFull: Evaluation of Spleen Swabs for Sensitive and High-Throughput Detection of Classical Swine Fever Virus.
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            NameFull: Hochman, Orie
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            – D: 01
              M: 08
              Text: Aug2025
              Type: published
              Y: 2025
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