Evaluation of SARS-CoV-2 Detection Efficiency by qRT-PCR Assays, Mainly on New Variants in East Java.
Saved in:
| Title: | Evaluation of SARS-CoV-2 Detection Efficiency by qRT-PCR Assays, Mainly on New Variants in East Java. |
|---|---|
| Authors: | Vida Loka, Ega Reviera, Juniastuti, Megasari, Ni Luh Ayu, Fitrianingsih, Avin Ainur, Lusida, Maria Inge |
| Source: | Trends in Sciences; Sep2025, Vol. 22 Issue 9, p1-13, 13p |
| Subject Terms: | SARS-CoV-2, COVID-19, REVERSE transcriptase polymerase chain reaction, SARS-CoV-2 Omicron variant, COMMUNICABLE diseases |
| Abstract: | Coronavirus disease 2019 (COVID-19) is an infectious disease caused by the novel coronavirus, SARS-CoV-2, which led to a global pandemic. Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has undergone mutations, leading to the emergence of new variants worldwide, which may affect the accuracy of the diagnostic methods. This study aimed to identify the distribution of SARS-CoV-2 variants in East Java from 2021 to 2024 and analyze the mutation positions in the primer/probe targets of quantitative Reverse Transcription Polymerase Chain Reaction (qRTPCR). This study assessed the mismatches in WHO-recommended and other kits binding regions qRT-PCR assays against SARS-CoV-2 genome sequences from East Java through in-silico bioinformatics analysis. The results indicate that from 2021 to 2024, the distribution of SARS-CoV-2 variants in East Java has changed over time, including B.1.466.2, Alpha, Beta, Delta, and Omicron, along with various Omicron lineages. Primer and probe sequences from Sansure, Liferiver, US-CDC, EasyDiagnosis, and Pasteur displayed accurate matches (> 90%) with the SARS-CoV-2 sequences from East Java. On the other hand, many Omicron subvariants in East Java had high mismatches with the primer sequences from Charité-RdRp and CN-CDC-N. These findings highlight the importance of continuous surveillance of circulating variants and their mutations to ensure the effectiveness of diagnostics and response actions for emerging and re-emerging variants in the future. [ABSTRACT FROM AUTHOR] |
| Copyright of Trends in Sciences is the property of Walailak Journal of Science & Technology and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Complementary Index |
Nájsť tento článok vo Web of Science Be the first to leave a comment!